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Contents 1 June 1.1 6/10/13 1.2 6/12/13 1.3 6/14/13 1.4 6/17/13 1.5 6/19/13 1.6 6/21/13

June

6/10/13

Today we looked at a paper describing efficient ways to propagate large phage. They suggested agarose instead of agar, and at a concentration of 0.15% which is 1.5 g/L. Dr. Grose explained to us that agarose is more stable and forms larger pores, requiring a smaller amount of powder to produce a solid, stable gel. LB Agarose top agar can be plated onto normal LB bottom agar. We will make up some agarose top agar and see what size of plaques we get.

Wednesday we will need to start an overnight culture in a 250 mL flask (using 25 mL of LB broth). We will a piece of a small colony and put it in the flask along with 20 ug of adenine per mL. It will incubate on the shaker until class starts.

6/12/13

6/14/13

6/17/13

6/19/13

6/21/13