Team:BYU Provo/Notebook/Phage Purification/Fallexp/Period1/Dailylog

From 2013.igem.org

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We ran a T7 purification today to see if the regular WT T7 bands at a new location with our new CsCl stock.
We ran a T7 purification today to see if the regular WT T7 bands at a new location with our new CsCl stock.
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<font size="4"> '''9/11/13''' </font>
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- We went ahead and ran another phage purification procedure with the new phage suspension buffer created in [Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.910XPhageSuspensionBuffer| 9.9 10X Phage Suspension Buffer Creation]]
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: -[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.11CsClGradient| 9.11 CsCl Gradient]]
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Revision as of 20:54, 11 September 2013


Phage Purification September - October Notebook: September 1 - September 14 Daily Log



Overview
March-April
May-June
July-August
September-October


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9/4/13

- Went over the results of our T4 and T7 purification with the Large Phage and small phage group. - Updated wiki and discussed plans to have everything completed by the deadlines.

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9/6/13

Continued updating wiki.

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9/7/13

We ran a T7 purification today to see if the regular WT T7 bands at a new location with our new CsCl stock.

- 9.7 CsCl Gradient

AB DL AC


9/9/13

We decided that our problem T7 banding in our gradient could be due to faulty phage suspension buffer. We created a new 10X solution of it to do all further gradients.

- 9.9 10X Phage Suspension Buffer Creation

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9/11/13

- We went ahead and ran another phage purification procedure with the new phage suspension buffer created in [Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.910XPhageSuspensionBuffer| 9.9 10X Phage Suspension Buffer Creation]]

- 9.11 CsCl Gradient

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<< Previous Next >>