Team:BYU Provo/Notebook/Phage Purification/Springexp/Period1/Dailylog

From 2013.igem.org

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<font size="4"> '''5/1/13''' </font>
<font size="4"> '''5/1/13''' </font>
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Today we created a detailed tentative plan to follow for the rest of the semester. https://docs.google.com/document/d/1ffqtGWjGOqEuOgNd46yK38lzkzMVdoCgXPcxMcWfCcc/edit?usp=sharing We went through the protein purification procedure to figure out the times that each step would take. https://docs.google.com/document/d/1W56-4bIIKsWBDtxsY2VgwV1q5BTo2nIXmrOLuqA0U9A/edit?usp=sharing
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: Today we created a detailed tentative plan to follow for the rest of the semester. https://docs.google.com/document/d/1ffqtGWjGOqEuOgNd46yK38lzkzMVdoCgXPcxMcWfCcc/edit?usp=sharing We went through the protein purification procedure to figure out the times that each step would take. https://docs.google.com/document/d/1W56-4bIIKsWBDtxsY2VgwV1q5BTo2nIXmrOLuqA0U9A/edit?usp=sharing
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<font size="4"> '''6/5/13''' </font>
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<font size="4"> '''5/3/13''' </font>
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- Extracted the phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/CsClGradientPhagePurification|6.3 CsCl Gradient Phage Purification]] using a syringe and a needle.  
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: We created an Excel sheet to calculate the amounts of each reagent for the phage purification experiment we will be running on Monday. This will allow for quick and  easy calculations of reagents needed each time we run the experiment.
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: Only extracted T4 because the T7 gradient was destroyed in the refrigerator.
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: Extracted the T4 band as well as debris from the bottom as the gradient was partially destroyed.
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: Placed these in the refrigerator.
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- Prepared stocks of bacteria for future use.
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: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.5 Bacteria Cultures|6.5 Bacteria Cultures]]
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<font size="4"> '''3/20/13''' </font>
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<font size="4"> '''5/5/13''' </font>
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: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.5 Bacteria Cultures|6.5 Bacteria Cultures]]

Revision as of 23:55, 7 June 2013


Phage Purification May - June Notebook: May 1 - May 12 Daily Log



Overview
March-April
May-June
July-August
September-October


5/1/13

Today we created a detailed tentative plan to follow for the rest of the semester. https://docs.google.com/document/d/1ffqtGWjGOqEuOgNd46yK38lzkzMVdoCgXPcxMcWfCcc/edit?usp=sharing We went through the protein purification procedure to figure out the times that each step would take. https://docs.google.com/document/d/1W56-4bIIKsWBDtxsY2VgwV1q5BTo2nIXmrOLuqA0U9A/edit?usp=sharing


5/3/13

We created an Excel sheet to calculate the amounts of each reagent for the phage purification experiment we will be running on Monday. This will allow for quick and easy calculations of reagents needed each time we run the experiment.


5/5/13

6.5 Bacteria Cultures



3/22/13


3/25/13