Team:BYU Provo/Notebook/Phage Purification/Springexp/Period4/Dailylog

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<font size="4"> '''6/3/13''' </font>
 
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- Ran the CsCl purification step.
 
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/CsClGradientPhagePurification|6.3 CsCl Gradient Phage Purification]]
 
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- Next class we will attempt extracting the phage from the gradient.
 
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<font size="4"> '''6/5/13''' </font>
 
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- Extracted the phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/CsClGradientPhagePurification|6.3 CsCl Gradient Phage Purification]] using a syringe and a needle. 
 
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: Only extracted T4 because the T7 gradient was destroyed in the refrigerator.
 
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: Extracted the T4 band as well as debris from the bottom as the gradient was partially destroyed.
 
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: Placed these in the refrigerator.
 
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- Prepared stocks of bacteria for future use.
 
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: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.5 Bacteria Cultures|6.5 Bacteria Cultures]]
 
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<font size="4"> '''6/7/13''' </font>
 
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: Made 1 L of 10 X phage suspension buffer.
 
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: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.7 Phage Suspension Buffer Preparation|6.7 Phage Suspension Buffer Preparation]]
 
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<font size="4"> "'6/14/13'" </font>
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- Worked on updating the website.
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Revision as of 20:10, 17 June 2013


Phage Purification May - June Notebook: June 10 - June 23 Daily Log



Overview
March-April
May-June
July-August
September-October


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6/10/13

- Prepared RNase A for future use.

6.10 RNase A Preparation

- Prepared T4 and T7 cultures.

6.10 Phage Preparation


6/12/13

- Began first step of purification process using phage from 6.10 Phage Preparation.

6.12 PEG Purification


"'6/14/13'"

- Worked on updating the website.


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