Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/6.7 Phage Suspension Buffer Preparation
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(Created page with "{{TeamBYUProvo}} <br> {| width="100%" | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Phage Purification May - June Notebook: Experiments'''</font> <br>...") |
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- | : [[Team:BYU_Provo/ | + | : [[Team:BYU_Provo/Phage_Purification|Overview]] |
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]] | : [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]] | ||
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- | <font size="5"> '''6. | + | <font size="5"> '''6.7 Phage Suspension Buffer Purification''' </font> |
<br> | <br> | ||
'''I) Purpose''' | '''I) Purpose''' | ||
- | : | + | : Create 1 L of 10X Phage Supension Buffer stock. |
'''II) Expected Outcome''' | '''II) Expected Outcome''' | ||
- | : | + | : Clean, usable stock of phage suspension buffer. |
'''III) Reagants Used''' | '''III) Reagants Used''' | ||
- | : | + | : NaCl |
- | : | + | : MgCl2 |
- | + | : Tris-HCl | |
'''IV) Actual Procedure''' | '''IV) Actual Procedure''' | ||
- | : | + | : Put 12.4 g Tris-HCl in a 1 L flask. Added 1 L H2O. Titrated to 7.2 pH using 50% NaOH. (.1 M Tris-HCl) |
- | : | + | : Added 58.44 g NaCl (1M) |
- | : | + | : Added 9.52 g MgCl2 (.1M) |
+ | : Autoclaved and stored in a sealed bottle for use. | ||
'''V) Results''' | '''V) Results''' | ||
- | : | + | : Created 1 L of 10X phage suspension buffer |
</font> | </font> |
Revision as of 22:01, 7 June 2013
Phage Purification May - June Notebook: Experiments
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6.7 Phage Suspension Buffer Purification
I) Purpose
II) Expected Outcome
III) Reagants Used
IV) Actual Procedure
V) Results
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