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Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period10/Dailylog
From 2013.igem.org
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- Performed a cesium chloride gradient purification on T7 mutated phage from the small phage team. | - Performed a cesium chloride gradient purification on T7 mutated phage from the small phage team. | ||
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Revision as of 21:11, 7 August 2013
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8/2/13 - Performed the spot test in 7.29 Mutagen Concentration Test - Sixth Protocol.
8/5/13 - Because the top agar used in yesterday's spot was contaminated, we decided to redo this step. Also, because most of the spot tests went down to -6, we performed further dilution for the next testing to get a more accurate idea of phage concentration. - Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration. - Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in 8.2 Modeling Phage Plaque Sizes - Experiment 1.
8/7/13 - Performed a cesium chloride gradient purification on T7 mutated phage from the small phage team.
8/4/13 - Started E coli liquid culture over night
8/5/13 - Performed Phage viability/infection test for 8.2 Modeling Phage Plaque Sizes - Experiment 1
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