Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Dailylog

5/28/13

- Started three 8mL E coli BL21 liquid culture at around 4pm.

5/29/13

- Continued 5.20 Mutagen Concentration Experiment

- Prepared sample for sequencing. This is done as part of 5.20 T7 Minor Capsid Protein PCR

- Dr. Studier responded to our email today! We also received the E coli stains containing plasmids with clone T7 genes. WE CAN START SITE DIRECTED MUTAGENESIS SOON!

5/30/13

- Plates from yesterday are taken out of incubation at around 4:00pm

5/31/13

- Discussed results for 5.20 Mutagen Concentration Experiment -> will need to try this one more time with minor adjustment to experimental design: plate -4 dilution using x6 and x8 top agar.

- Discussed plans for next week.

- Made new LB and x6 top agar.

5/17/13

- Determined that all LB plates from 5.8 had contamination

- Poured new LB plates

- Made x8 top agar

5/18/13

- Stacked up the LB plates made yesterday. No obvious sign of contamination seen.

- Threw away the 5.8 LB plates (the ones with contamination).

5/19/13

- Started two 5mL of E coli BL21 overnight

- Designed procedure for applying mutagen and selecting for T7

5/20/13

- Performed T7 Mutagen Concentration Test