Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol
From 2013.igem.org
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2) Applying the mutagen (7.29) | 2) Applying the mutagen (7.29) | ||
- | : - Label 10 test tubes LB:C, LB:0, LB:100, LB:200, LB: 500, M9:C, M9:0, M9:100, M9: 200, and M9: 500. Add | + | : - Label 10 test tubes LB:C, LB:0, LB:100, LB:200, LB: 500, M9:C, M9:0, M9:100, M9: 200, and M9: 500. Add 9.8mL of LB, 40ul of adenine solution into each test tube. Then add 200ul of BL21 overnight into each test tube. Incubate on the shaker at 37 Celsius. |
: - Make the M9+ suspension medium and supplement it with the appropriate amount of uracil and adenine. In this case, 200uL of adenine solution and 400uL of uracil solution was add 50mL of M9+. | : - Make the M9+ suspension medium and supplement it with the appropriate amount of uracil and adenine. In this case, 200uL of adenine solution and 400uL of uracil solution was add 50mL of M9+. | ||
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: - Place all the tubes on the shaker at 37 Celsius for 30 minutes. | : - Place all the tubes on the shaker at 37 Celsius for 30 minutes. | ||
- | : - Remove the tubes and add | + | : - Remove the tubes and add 10ul of T7 phage from the "10ul 7.19 stock" to each tube, except for the tubes with a "C" on them. |
: - Incubate all the tubes on the shaker at 37 Celsius for 80 minutes. | : - Incubate all the tubes on the shaker at 37 Celsius for 80 minutes. |
Revision as of 21:40, 29 July 2013
Small Phage July - August Notebook: Experiments
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7.29 Mutagen Concentration Test - Sixth Protocol
I) Purpose
II) Expected Outcome
III) Reagents Used
IV) Procedure 1) Overnight (The day before) (7.28)
2) Applying the mutagen (7.29)
VI) Conclusion
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