Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size
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* E coli BL21 supports the growth of bacteriophage T2, T3 30, and T7. On the other hand, E coli B and E coli W3110 support bacteriophage T1, T2 30, T3 30, T4, and T7. Other phage stocks either do not infect the bacteria strains tested or have died during storage in the fridge. | * E coli BL21 supports the growth of bacteriophage T2, T3 30, and T7. On the other hand, E coli B and E coli W3110 support bacteriophage T1, T2 30, T3 30, T4, and T7. Other phage stocks either do not infect the bacteria strains tested or have died during storage in the fridge. | ||
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* Because E coli B is the strain routinely used for the Large Phage Group's experiments, we decided to base our modeling on E coli B. | * Because E coli B is the strain routinely used for the Large Phage Group's experiments, we decided to base our modeling on E coli B. | ||
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* T1 formed plaques up to -3; T2 -5; T3 -6; T4 -7; T7 -6. | * T1 formed plaques up to -3; T2 -5; T3 -6; T4 -7; T7 -6. | ||
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4) Preliminary titer test | 4) Preliminary titer test | ||
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* No contamination this time. Yay! | * No contamination this time. Yay! | ||
- | * Results are similar to those observed in Preliminary Titer Test. T4 < T1 < T7 < T2 < T3. T2 and T7 are not behaving as expected. As a T4 like phage, T2 is forming plaques that are too big. Based on our experience working with T7, the observed | + | [[File:BYUSPMP-3.JPG|400px|center|link=]] |
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+ | * Results are similar to those observed in Preliminary Titer Test. T4 < T1 < T7 < T2 < T3. T2 and T7 are not behaving as expected. As a T4 like phage, T2 is forming plaques that are too big. Based on our experience working with T7, the observed plaques from this time are too small. | ||
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Latest revision as of 15:38, 9 September 2013
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8.2 Modeling Phage Plaque Sizes - Preliminary Experiments
I) Purpose
II) Expected Outcome
III) Reagents Used
IV) Procedure 1) E coli strain preparation (8.2-8.5)
2) Phage viability/infection test (8.5)
3) Spot test to estimate phage titer (8.7)
4) Preliminary titer test (8.10)
5) Titer repeat (8.12)
V) Results 1) E coli strain preparation
2) Phage viability/infection test
3) Spot test to estimate phage titer
4) Preliminary titer test
5) Titer repeat
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