Team:Dundee/Parts/Ourbiobricks

We have submitted two BioBricks to the Registry of Standard Biological Parts that will hopefully be of use to future teams and projects.

Protein Phosphatase 1

Human Protein Phosphatase 1 (PP1) is a protein from the family of serine/threonine phosphatases, we have used it as a microcystin binding protein however it regulates many processes in the body therefore it may be used in many other ways.

OmpC-GFP reporter construct.

This reporter gene was constructed in order to test the EnvZ receptor. It consists of three parts: The ompC promoter (which is regulated by OmpR), a ribosome binding site and the GFP gene. This will ensure that upon activation of the EnvZ receptor, cells will start expressing GFP. This part has been verified to work in this way.

Due to the time constraints we have been unable to submit the three BioBricks described below however, we hope to submit those once the registry re-opens again.

The signal sequence of MalE

Signal allowing for protein transport via the general secretory pathway (Sec) in E. coli

The signal sequence of PrsA

Signal allowing for protein transport by the general secretory pathway (Sec) in B. subtilis. This signal sequence also contains a lipobox, facilitating anchoring to the outer leaflet of the cytoplasmic membrane.

The signal sequence of TorA.

Signal allowing for protein transport via the E. coli Twin Arginine Translocation pathway (Tat) into the periplasm.