Team:EPF Lausanne/Calendar/10 August 2013

From 2013.igem.org

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<font size = "4"> Cell Surface Display </font> <BR>
<font size = "4"> Cell Surface Display </font> <BR>
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''Moving on''  
''Moving on''  
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<br>Miniprep of cells transformed with ''INP_Strep-Dead'' gibson product.
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<br>-Miniprep of cells transformed with ''INP_Strep-Dead'' gibson product.
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<br> DpnI digestion of Streptavidin iGEM (BBa_K283010) PCR product.
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<br> -DpnI digestion of Streptavidin iGEM (BBa_K283010) PCR product.
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<br> Gibson assembly of INP amplified construct and streptavidin iGEM PCR purified product.
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<br> -Gibson assembly of INP amplified construct and streptavidin iGEM PCR purified product.
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<br> Plating of Streptavdin alive received from Oxford University.
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<br> -Plating of Streptavdin alive received from Oxford University.
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<font size = "4"> Sensing </font> <BR>
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<font size = "4"> Sensing-Effector</font> <BR>
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'''Making a glycerol Stock''' <BR>
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''Making a glycerol Stock'' <BR>
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We made a 50% glycerol stock of the Bacteria that we innoculated the day before.
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-We made a 50% glycerol stock of the Bacteria that we inoculated the day before.
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Latest revision as of 21:46, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Moving on
-Miniprep of cells transformed with INP_Strep-Dead gibson product.
-DpnI digestion of Streptavidin iGEM (BBa_K283010) PCR product.
-Gibson assembly of INP amplified construct and streptavidin iGEM PCR purified product.
-Plating of Streptavdin alive received from Oxford University.


Sensing-Effector

Making a glycerol Stock
-We made a 50% glycerol stock of the Bacteria that we inoculated the day before.