Team:Evry/Protocoles/04

From 2013.igem.org

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<h2> Antibiotiques </h2>
<h2> Antibiotiques </h2>
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Carbénicilline:
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Carbénicilline:<br>
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Kanamycine:
+
Kanamycine:<br>
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Chloramphénicole:
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Chloramphénicole:<br><br>
<h2> LB and LB Agar </h2>
<h2> LB and LB Agar </h2>

Revision as of 11:28, 31 July 2013

Solutions, Milieux et boîtes de Pétri

Antibiotiques

Carbénicilline:
Kanamycine:
Chloramphénicole:

LB and LB Agar

Mix 20 g of LB Broth in 1L of distilled water in a bottle of 1L. Put in autoclave overnight.
Mix 17,5 g of LB Agar and in 500 mL of distilled water in a bottle of 1L. Put in autoclave overnight.

CaCl2 and CaCl2+glycérol

Tris HCl