Team:Evry/Protocoles/04

From 2013.igem.org

(Difference between revisions)
Line 12: Line 12:
Kanamycine:<br>
Kanamycine:<br>
-
Chloramphénicole:<br><br>
+
Chloramphénicole:<br>
<h2> LB and LB Agar </h2>
<h2> LB and LB Agar </h2>

Revision as of 11:29, 31 July 2013

Solutions, Milieux et boîtes de Pétri

Antibiotiques

Carbénicilline:
Kanamycine:
Chloramphénicole:

LB and LB Agar

Mix 20 g of LB Broth in 1L of distilled water in a bottle of 1L. Put in autoclave overnight.
Mix 17,5 g of LB Agar and in 500 mL of distilled water in a bottle of 1L. Put in autoclave overnight.

CaCl2 and CaCl2+glycérol

Tris HCl