Team:Exeter/Parts
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<groupparts>iGEM013 Exeter</groupparts> | <groupparts>iGEM013 Exeter</groupparts> |
Revision as of 18:13, 2 October 2013
This page will document the parts that Exeter iGEM 2013 submit to the biobrick registry.
Our aim is to submit a composite biobrick for E. coli which will allow control three genes of your choice using Red, Green and Blue (RGB) light. We have successful sent in the composite part for the Green Light Module ([1]). We also aim to create constituent bio-bricks for red/blue/green light input matching cyan/yellow/magenta pigments respectively. We have managed to submit a brick for the right light module (includes the cyan pigment, see [2]). We will also submit a constituent bio-brick for red light input with cut sites to allow input of your favourite gene ([3]). See our project section for details about our bio-bricks.
We plan to extensively characterise our system and provide a mathematical model of our system for quantative control of outputs. See our modelling section.
Table including all of the parts we are hoping to utilise
Part Code and link to Registry page | Name of Part |
---|---|
BBa_K608002 | Strong promoter and strong RBS |
BBa_I712074 | T7 promoter |
BBa_B0015 | Terminator |
BBa_Q04510 | lambda cI inverter system |
BBa_K322124 | Cph8 red light sensor with RBS, version one |
BBa_K592018 | Cph8 red light sensor with RBS, version two |
BBa_K098011 | OmpR, the protein which communicates between the red light sensor and the cyan pigment gene |
BBa_R0082 | OmpR specific promoter, only allows transcription when OmpR-P is present |
BBa_K322122 | Cyan pigment, version one (composed of HO1 and pcyA, makes phycocyanobillin) |
BBa_K909000 | Cyan pigment, version one, includes stronger RBS than BBa_K322122 |
BBa_K592011 | Cyan pigment, version two |
BBa_K864404 | Cyan pigment, version two, includes a promoter and RBS |
BBa_K592022 | Cyan pigment, version two, includes a alternative promoter and RBS |
BBa_K592004 | YF1 blue light sensor |
BBa_K592005 | FixJ, the protein which communicates between the blue light sensor and the yellow pigment gene |
BBa_K592006 | FixJ specific promoter, only allows transcription when FixJ-P is present |
BBa_K592010 | Yellow pigment, version one |
BBa_S05058 | Yellow pigment, version one, with added RBS |
BBa_K592001 | CcaS green light sensor |
BBa_K592002 | CcaR, the protein which communicates between the green light sensor and the lambda inverter system |
BBa_K592017 | Combined CcaS and CcaR with RBS before each |
BBa_K592003 | CcaR specific promoter, only allows transcription when CcaR-P is NOT present |
BBa_K592012 | Magenta pigment |
BBa_KJ04450 | RFP control |
BBa_I746915 | Superfolder GFP |
<groupparts>iGEM013 Exeter</groupparts>