Team:Goettingen/NoteBook

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Revision as of 06:43, 18 August 2013

The beast and its Achilles heel:

 A novel target to fight multi-resistant pathogenic bacteria


Timeline

June
05th

Preparation of the medium, antibioticks, Transformation.

Preparation of Antibiotic Stocks
  • 1000x Ampicillin 10 1mL Stocks (EPs in the red box in -20 freezer)
  • 1000x Chloramphenicol 10mL Stock (Falcon in Freezer)
Media preparation
  • 250ml*4 LB media with Cm
  • 250ml*1 LB media with Amp
Primer design
Code Name Description
iGEM_36 DarR operator sequence + biobrick prefix
iGEM_37 DarR operator sequence + biobrick sufix

Transformation
Biobrick entry numberMark
BBa_J23117 part 1
BBa_J23116 part 2
BBa_J23110 part 3
BBa_J23118 part 4
BBa_J61101 part 5
BBa_BE0240-Cm part 6 -- Cm
BBa_B0015 part 7 -- Cm
BBa_B0034 part 8
Resuspended but not transformed
Entry number location on kit
BBa_E0204-Amp P5 12 M
BBa_QO3121 P5 20 N
04th

Find the correct DNA sequence of DarR and primer design

Code Name Description
iGEM_32 Forward primer for DarR ORF amplification, with biobrick prefix
iGEM_33 Reverse primer for DarR ORF amplification, with biobrick sufix
iGEM_34 Forward primer for DarR ORF PCR amplification sequencing.
iGEM_35 Reverse primer for DarR ORF PCR amplification sequencing.