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The beast and its Achilles heel:
A novel target to fight multi-resistant pathogenic bacteria
Timeline
June
19th
PCR using primer iGEM 36,37 for construction of DarR operator biobrick.
Same recipe as yesterdays PCR, rest the protocol,
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98.5 |
5min |
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|
98.5 |
30s |
10 cycle |
20cycle |
30cycle |
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61.0 |
30s |
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72.0 |
2min |
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|
72.0 |
10min |
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Ctrl: no enzyme
Increase the gel concentration to 4%.
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