Team:Goettingen/Team/Array

From 2013.igem.org

Revision as of 14:11, 16 September 2013 by Demarcia (Talk | contribs)

The beast and its Achilles heel:

 A novel target to fight multi-resistant pathogenic bacteria



Array Team

By using microarray analysis we would like to determine, which genes are regulated by ci-di-AMP. In order to determine this, we are using three different Bacillus subtilus strains. The first strain contains all three active diadenylatecyclase disA, cdaA, cdaS. The second strain is mutant strain (ΔdisA). The third strain is a double mutant strain, which only contains one active diadenylatecyclase (ΔdisA, ΔcdaA). The microarray analysis will then show, which genes are expressed under different intracellular ci-di-AMP concentrations. Furthermore, we would like to create a triple knock-out mutant (ΔdisA, ΔcdaA, ΔcdaS). We are trying to create this triple knock-out mutant via a feeding experiment.

 

Previous Next