Team:Heidelberg/DesignTest

From 2013.igem.org

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Here we are going to test our wiki design.
Here we are going to test our wiki design.
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==Week 7==
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=== (Konrad)===
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==== fragment amplification ====
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==> f1:  (all)
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* 14.6 µl H2O
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* 25 µl Phusion Flash
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* 2x 0.5 µl Primer (NI01,NI06)
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* 0.4 µl template hasi
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{| class="wikitable"
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|-
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! Cycles !! temperature [°C] !! Time [s]
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|-
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| 1 || 98 || 10
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|-
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| rowspan="3"| 30 || 98 || 1
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|-
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| 66 || 5
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|-
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| 72 || 120
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|-
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| 1 || 72 || 420
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|-
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| 1  || 4 || inf
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|}
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[[File:Heidelberg teamfoto.jpg|400px|thumb|left|PCR for amplification of  (all); lane 11]]
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<br\>  <br\>  <br\>  <br\>  <br\>  <br\>  <br\>  <br\>  <br\>  <br\>  <br\>  <br\>  <br\>  <br\>  <br\>  <br\> 
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<br\>  <br\>  <br\>  <br\>
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:Gel purification (50µl): by accident did not take 3 but 1 Volume buffer QG
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:Labelling fragements: 1 I and 1 II (from different lanes)
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</html>
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{{:Team:Heidelberg/Templates/EndPage}}
{{:Team:Heidelberg/Templates/EndPage}}

Revision as of 09:29, 12 August 2013

PHILOSOPHER'S STONE

igem-2013

{{:Team:Heidelberg/Templates/{{{2}}}}}
iGEM Main page

Here we are going to test our wiki design.

==Week 7== === (Konrad)=== ==== fragment amplification ==== ==> f1: (all) * 14.6 µl H2O * 25 µl Phusion Flash * 2x 0.5 µl Primer (NI01,NI06) * 0.4 µl template hasi {| class="wikitable" |- ! Cycles !! temperature [°C] !! Time [s] |- | 1 || 98 || 10 |- | rowspan="3"| 30 || 98 || 1 |- | 66 || 5 |- | 72 || 120 |- | 1 || 72 || 420 |- | 1 || 4 || inf |} [[File:Heidelberg teamfoto.jpg|400px|thumb|left|PCR for amplification of (all); lane 11]] :Gel purification (50µl): by accident did not take 3 but 1 Volume buffer QG :Labelling fragements: 1 I and 1 II (from different lanes)

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