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Team:Heidelberg/DesignTest
From 2013.igem.org
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Revision as of 09:30, 12 August 2013
Team
Project
Notebook
Parts
Cool tool
Human Practice
Safety
iGEM Main page
Here we are going to test our wiki design.
Week 7
(Konrad)
fragment amplification
==> f1: (all)
- 14.6 µl H2O
- 25 µl Phusion Flash
- 2x 0.5 µl Primer (NI01,NI06)
- 0.4 µl template hasi
| Cycles | temperature [°C] | Time [s] |
|---|---|---|
| 1 | 98 | 10 |
| 30 | 98 | 1 |
| 66 | 5 | |
| 72 | 120 | |
| 1 | 72 | 420 |
| 1 | 4 | inf |
File:Heidelberg teamfoto.jpg
PCR for amplification of (all); lane 11
- Gel purification (50µl): by accident did not take 3 but 1 Volume buffer QG
- Labelling fragements: 1 I and 1 II (from different lanes)
