Team:Heidelberg/DesignTest

From 2013.igem.org

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Revision as of 09:31, 12 August 2013

PHILOSOPHER'S STONE

igem-2013

{{:Team:Heidelberg/Templates/{{{2}}}}}
iGEM Main page

Here we are going to test our wiki design.

Week 7

(Konrad)

fragment amplification

==> f1: (all)

  • 14.6 µl H2O
  • 25 µl Phusion Flash
  • 2x 0.5 µl Primer (NI01,NI06)
  • 0.4 µl template hasi


Cycles temperature [°C] Time [s]
1 98 10
30 98 1
66 5
72 120
1 72 420
1 4 inf
PCR for amplification of (all); lane 11





















Gel purification (50µl): by accident did not take 3 but 1 Volume buffer QG
Labelling fragements: 1 I and 1 II (from different lanes)

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