Team:Heidelberg/DesignTest

From 2013.igem.org

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==Week 7==
 
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=== (Konrad)===
 
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==== fragment amplification ====
 
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==> f1:  (all)
 
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* 14.6 µl H2O
 
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* 25 µl Phusion Flash
 
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* 2x 0.5 µl Primer (NI01,NI06)
 
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* 0.4 µl template hasi
 
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{| class="wikitable"
 
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|-
 
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! Cycles !! temperature [°C] !! Time [s]
 
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|-
 
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| 1 || 98 || 10
 
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|-
 
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| rowspan="3"| 30 || 98 || 1
 
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|-
 
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| 66 || 5
 
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|-
 
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| 72 || 120
 
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|-
 
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| 1 || 72 || 420
 
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|-
 
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| 1  || 4 || inf
 
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|}
 
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[[File:Heidelberg teamfoto.JPG|400px|thumb|left|PCR for amplification of  (all); lane 11]]
 
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<br\>  <br\>  <br\>  <br\>  <br\>  <br\>  <br\>  <br\>  <br\>  <br\>  <br\>  <br\>  <br\>  <br\>  <br\>  <br\> 
 
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<br\>  <br\>  <br\>  <br\>
 
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:Gel purification (50µl): by accident did not take 3 but 1 Volume buffer QG
 
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:Labelling fragements: 1 I and 1 II (from different lanes)
 
{{:Team:Heidelberg/Templates/EndPage}}
{{:Team:Heidelberg/Templates/EndPage}}

Revision as of 09:33, 12 August 2013

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