Team:Hong Kong HKUST/Wetlab

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<br><br><br><div id="slide"><center><h3 class="title">Wetlab</h3></center><p id="yo"</div>  
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<div id="interview"><h3>Logbook</h3><div id="pre-img"><img src="https://static.igem.org/mediawiki/2013/6/62/Presentation.png" style="padding-left:20px;padding-top:5px;width:80%;"><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/presentation"><img src="http://w1ggroup.com/devere/img/more_off.png" style="width:80%;height:40px;"></a></center></div><div id="pre-desc"><p id="pre-desc-text">Presentation is one way to share information about project and iGEM to other people, whether it is to fellow academics or to the general public. Find out more about our past and upcoming presentation on synthetic biology.</p></div></div>
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The wet lab started at the beginning of summer and lasted for three months. Our wet lab work is divided into four parts, namely: (1) cell viability and fatty acid quantification, (2) fatty acid sensing mechanism, (3) mitochondrial leader sequence, and (4) construction of glyoxylate shunt. During the summer, lab work such as primer design, digestion, and ligation was carried out extensively and useful data were collected. These can be retrieved from <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/notebook">Notebook</a> and <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/protocols">Protocols</a> pages for details of experimental flows. The BioBrick, researcher, environmental and university safety issues can be found in <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/safety">Safety</a> page. In terms of project process, some experiments could not be completed due to time constrains and they are outlined in <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/future">Future Work</a> page.
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<div id="presentation"><h3>Protocols</h3><div id="pre-img"><img src="https://static.igem.org/mediawiki/2013/e/ef/Globes.png" style="padding-left:20px;width:80%;padding-top:5px;"><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/cp"><img src="http://w1ggroup.com/devere/img/more_off.png" style="width:80%;height:40px;padding-top:5px;"></a></center></div><div id="pre-desc"><p id="pre-desc-text">This is a novel human practice project that we did. We thought it was necessary to gain information of synthetic biology in Asian countries to have a holistic view of synthetic biology around the world.</p></div></div>
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<div id="interview"><h3>Notebook</h3><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/notebook"><img src="https://static.igem.org/mediawiki/2013/c/cc/Note.png" style="padding-left:0px;margin-top:-8px;width:80%;"></a></center></div>
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<div id="presentation"><h3>Protocols</h3><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/protocols"><img src="https://static.igem.org/mediawiki/2013/1/1d/Protocols.png" style="padding-left:10px;padding-top:10px;width:60%;"></a></center></div>
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<div id="article"><h3>Safety</h3><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/safety"><img src="https://static.igem.org/mediawiki/2013/7/73/Safe.png" style="padding-left:0px;padding-top:-3px;width:75%;"></a></center></div></div>
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<div id="article"><h3>Safety</h3><div id="art-img"><img src="https://static.igem.org/mediawiki/2013/c/c0/Genetherapy.png" style="padding-left:20px;width=70%;padding-top:5px"><center><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/article/genet"><img src="http://w1ggroup.com/devere/img/more_off.png" style="width:80%;height:40px;padding-top:10px;"></a></center></div><div id="art-desc"><p id="art-desc-text">It is necessary to think about how our project could be actually applied. Since gene therapy was mentioned as a mean to deliver our DNA into human liver cells, we thought it was necessary to research on the current gene therapy technology.</p></div></div>
 
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<div id="interview1"><h3>Wetlab</h3>
 
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<div id="int-desc"><p id="int-desc-text">We have made several videos to inform the general public about synthetic biology and our project. We tried to make the contents relatively easy so that people with no biology background understand the content.</p></div></div>
 
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Latest revision as of 23:03, 27 September 2013




Wetlab


The wet lab started at the beginning of summer and lasted for three months. Our wet lab work is divided into four parts, namely: (1) cell viability and fatty acid quantification, (2) fatty acid sensing mechanism, (3) mitochondrial leader sequence, and (4) construction of glyoxylate shunt. During the summer, lab work such as primer design, digestion, and ligation was carried out extensively and useful data were collected. These can be retrieved from Notebook and Protocols pages for details of experimental flows. The BioBrick, researcher, environmental and university safety issues can be found in Safety page. In terms of project process, some experiments could not be completed due to time constrains and they are outlined in Future Work page.

Notebook

Protocols

Safety