Team:Hong Kong HKUST/data

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<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Wetlab">Wetlab</a>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Wetlab">Wetlab</a>
<ul>
<ul>
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<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/experiment">Experiments</a></li>
 
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/notebook">Notebook</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/notebook">Notebook</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/protocols">Protocols</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/protocols">Protocols</a></li>
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<br><br><br><div id="slide"><center><h3 class="title">Data Page</h3></center><br><br>
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<br><br><br><div id="slide"><center><h3 class="title">Data Page</h3></center><br>
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<p>This page shows all the parts we have made or used in our whole project. Click on the link below to see our constructs on the Registry of Biological Standard Part in detail. Click on main result page for an overview of our main result for those parts.<br>
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<p><font size=3><i>This page shows all the parts we have made or used in our whole project. Click on the links below to see our constructs on the Registry of Standard Biological Parts in detail. Click <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/results">here</a> to access our result page for an overview of our achievements with those parts.</i></font>
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   <br><br>
   <h3>How our system works:</h3><br>
   <h3>How our system works:</h3><br>
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   <h3>Data for Our Favorite New Parts:</h3>
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   <h3>Data For Our Favorite New Parts:</h3>
<ul>
<ul>
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   <li><strong>Mitochondria leader sequence, BBa_K1119001</strong> : for directing protein into mitochondria</li>
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   <li><strong>Mitochondrial leader sequence, <a href="http://parts.igem.org/Part:BBa_K1119001">BBa_K1119001</a></strong>: for directing proteins to the mitochondria. When the mitochondrial leader sequence is attached, glyoxylate enzymes AceA and AceB can be led into mitochondria to facilitate glyoxylate shunt.</li>
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  <li>In the presence of mitochondria leader sequence, glyoxylate enzymes AceA and AceB can be led into mitochondria to introduce glyoxylate shunt.</li>
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   <li><strong>Cytomegalovirus promoter (CMV Promoter), <a href="http://parts.igem.org/Part:BBa_K1119006">BBa_K1119006</a></strong>: constitutive mammalian promoter. With the CMV promoter, constitutive expression of the glyoxylate genes <i>aceA</i> and <i>aceB</i> can be realized.</li>
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   <li><strong>Cytomegalovirus promoter (CMV), BBa_K1119006</strong>: constitutive mammalian promoter</li>
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<li><strong>Human Elongation Factor-1alpha promoter (EF-1alpha Promoter), <a href="http://parts.igem.org/Part:BBa_K1119006">BBa_K1119010</a></strong>: constitutive mammalian promoter. With the EF-1alpha promoter, constitutive expression of the glyoxylate genes <i>aceA</i> and <i>aceB</i> can be realized.</li>
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  <li>With the pCMV, glyoxylate genes AceA and AceB can be driven to be expressed.</li>
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     <h3>Data for Pre-existing Parts:</h3>
     <h3>Data for Pre-existing Parts:</h3>
     <ul>
     <ul>
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       <li><strong>Kozak-XbaI-NLS-6His-stop, BBa_J176171</strong> (Haynes Lab, 2007):  used as a backbone for pfadBA and FABP.</li>
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       <li><strong>Kozak-XbaI-NLS-6His-Stop, <a href="http://parts.igem.org/Part:BBa_J176171">BBa_J176171</a></strong> (Haynes Lab, 2007): used as a backbone for P<sub><i>fadBA </i></sub>and P<sub><i>fabp1</i></sub>.</li>
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       <li><strong>hGH-PolyA, </strong><strong>BBa_K404108</strong></a><strong> </strong>(Freiburg Bioware iGEM, 2010):  PolyA tail was added after the GFP sequence in the construct of <strong>BBa_K1119008 </strong>and<strong> BBa_K1119009 </strong>for characterization of MLS, which gave us the expected results.</li>
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       <li><strong>hGH-PolyA, </strong><strong><a href="http://parts.igem.org/Part:BBa_K404108">BBa_K404108</a></strong></a><strong> </strong>(Freiburg Bioware iGEM, 2010): hGH PolyA tail was added after the GFP sequence in the construct of <strong><a href="http://parts.igem.org/Part:BBa_K1119008">BBa_K1119008 </a></strong>and<strong> <a href="http://parts.igem.org/Part:BBa_K1119009">BBa_K1119009</a> </strong>for characterization of MLS, which gave us the expected results.</li>
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       <li><strong>GFP BBa_K648013</strong>(Penn-State iGEM, 2011): green fluorescent protein reporter (E0040) with standard 25 prefix and suffix was used as a reporter for characterization of MLS and pCMV.</li>
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       <li><strong>GFP <a href="http://parts.igem.org/Part:BBa_K648013">BBa_K648013</a></strong>(Penn-State iGEM, 2011): green fluorescent protein reporter (<a href="http://parts.igem.org/Part:BBa_E0040"><b>BBa_E0040</b></a>) with RFC 25 assembly standard prefix and suffix was used as a reporter for characterization of MLS and the CMV promoter.</li>
     </ul>
     </ul>
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<h3>We've Also Characterized the Following Parts</h3>
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<h3>We Also Characterized the Following Parts</h3>
   <ul>
   <ul>
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     <li><strong>CMV regulated GFP, BBa_K1119008: </strong>By assembling pCMV with GFP-PolyA, and transfection into HEK293FT cells, GFP can be successfully expressed.</li>
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     <li><strong>CMV promoter-GFP, <a href="http://parts.igem.org/Part:BBa_K1119008">BBa_K1119008</a>: </strong>CMV promoter was assembled with GFP-hGH PolyA. Following transfection of the construct into HEK293FT cells, GFP was successfully expressed.</li>
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     <li><strong>Mitochondria Leader Sequence – CMV promoter, BBa_K1119009</strong>: By assembling pCMV with MLS and GFP-PolyA, GFP can be successfully expressed and directed into mitochondria after transfection into HEK293FT cells.</li>
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     <li><strong>CMV promoter-Mitochondrial Leader Sequence fused with GFP , <a href="http://parts.igem.org/Part:BBa_K1119009">BBa_K1119009</a></strong>: CMV promoter assembled with MLS and GFP-hGH PolyA. Following transfection of the construct into HEK293FT cells, GFP was successfully expressed and directed into mitochondria.</li>
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<li><strong>EF-1alpha promoter-GFP, <a href="http://parts.igem.org/Part:BBa_K1119011">BBa_K1119011</a>: </strong>EF-1alpha promoter was assembled with GFP-hGH PolyA. Following transfection of the construct into HEK293FT cells, GFP was successfully expressed.</li>
   </ul>
   </ul>
   <br><br><br>
   <br><br><br>

Latest revision as of 13:20, 28 October 2013




Data Page


This page shows all the parts we have made or used in our whole project. Click on the links below to see our constructs on the Registry of Standard Biological Parts in detail. Click here to access our result page for an overview of our achievements with those parts.

How our system works:




Data For Our Favorite New Parts:

  • Mitochondrial leader sequence, BBa_K1119001: for directing proteins to the mitochondria. When the mitochondrial leader sequence is attached, glyoxylate enzymes AceA and AceB can be led into mitochondria to facilitate glyoxylate shunt.
  • Cytomegalovirus promoter (CMV Promoter), BBa_K1119006: constitutive mammalian promoter. With the CMV promoter, constitutive expression of the glyoxylate genes aceA and aceB can be realized.
  • Human Elongation Factor-1alpha promoter (EF-1alpha Promoter), BBa_K1119010: constitutive mammalian promoter. With the EF-1alpha promoter, constitutive expression of the glyoxylate genes aceA and aceB can be realized.


Data for Pre-existing Parts:

  • Kozak-XbaI-NLS-6His-Stop, BBa_J176171 (Haynes Lab, 2007): used as a backbone for PfadBA and Pfabp1.
  • hGH-PolyA, BBa_K404108 (Freiburg Bioware iGEM, 2010): hGH PolyA tail was added after the GFP sequence in the construct of BBa_K1119008 and BBa_K1119009 for characterization of MLS, which gave us the expected results.
  • GFP BBa_K648013(Penn-State iGEM, 2011): green fluorescent protein reporter (BBa_E0040) with RFC 25 assembly standard prefix and suffix was used as a reporter for characterization of MLS and the CMV promoter.


We Also Characterized the Following Parts

  • CMV promoter-GFP, BBa_K1119008: CMV promoter was assembled with GFP-hGH PolyA. Following transfection of the construct into HEK293FT cells, GFP was successfully expressed.
  • CMV promoter-Mitochondrial Leader Sequence fused with GFP , BBa_K1119009: CMV promoter assembled with MLS and GFP-hGH PolyA. Following transfection of the construct into HEK293FT cells, GFP was successfully expressed and directed into mitochondria.
  • EF-1alpha promoter-GFP, BBa_K1119011: EF-1alpha promoter was assembled with GFP-hGH PolyA. Following transfection of the construct into HEK293FT cells, GFP was successfully expressed.