Team:Hong Kong HKUST/modules

From 2013.igem.org

(Difference between revisions)
(Created page with "<html> <head> <script src="http://ajax.googleapis.com/ajax/libs/jquery/1.10.2/jquery.min.js"> </script> <script type="text/javascript"> $(document).ready(function(){ $("ul.first...")
 
(58 intermediate revisions not shown)
Line 12: Line 12:
</script>   
</script>   
 +
<script>
 +
  $(document).ready(function()
 +
  {
 +
  $('#cell-small-03').mouseover(function(){
 +
    $('.info1').stop().hide();
 +
    $('#presentation').stop().show();
 +
  });
 +
  $('#cell-small-06').mouseover(function(){
 +
    $('.info1').stop().hide();
 +
    $('#article').stop().show();
 +
  });
 +
  $('#cell-small-09').mouseover(function(){
 +
    $('.info1').stop().hide();
 +
    $('#article2').stop().show();
 +
  });
 +
});
 +
</script>
<script>
<script>
   $(document).ready(function()
   $(document).ready(function()
Line 29: Line 46:
});
});
</script>
</script>
 +
 +
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
 +
<!-- Save for Web Styles (cell small.psd) -->
 +
<style type="text/css">
 +
<!--
 +
 +
#Table_01 {
 +
position:absolute;
 +
left:22px;
 +
top:375px;
 +
width:600px;
 +
height:400px;
 +
}
 +
 +
#cell-small-01 {
 +
position:absolute;
 +
left:0px;
 +
top:0px;
 +
width:600px;
 +
height:128px;
 +
}
 +
 +
#cell-small-02 {
 +
position:absolute;
 +
left:0px;
 +
top:128px;
 +
width:121px;
 +
height:272px;
 +
}
 +
 +
#cell-small-03 {
 +
position:absolute;
 +
left:121px;
 +
top:128px;
 +
width:95px;
 +
height:84px;
 +
}
 +
 +
#cell-small-04 {
 +
position:absolute;
 +
left:216px;
 +
top:128px;
 +
width:117px;
 +
height:84px;
 +
}
 +
 +
#cell-small-05 {
 +
position:absolute;
 +
left:333px;
 +
top:128px;
 +
width:70px;
 +
height:159px;
 +
}
 +
 +
#cell-small-06 {
 +
position:absolute;
 +
left:403px;
 +
top:128px;
 +
width:122px;
 +
height:159px;
 +
}
 +
 +
#cell-small-07 {
 +
position:absolute;
 +
left:525px;
 +
top:128px;
 +
width:75px;
 +
height:272px;
 +
}
 +
 +
#cell-small-08 {
 +
position:absolute;
 +
left:121px;
 +
top:212px;
 +
width:120px;
 +
height:75px;
 +
}
 +
 +
#cell-small-09 {
 +
position:absolute;
 +
left:241px;
 +
top:212px;
 +
width:92px;
 +
height:75px;
 +
}
 +
 +
#cell-small-10 {
 +
position:absolute;
 +
left:121px;
 +
top:287px;
 +
width:404px;
 +
height:113px;
 +
}
 +
 +
-->
 +
</style>
 +
 +
<style type="text/css">
<style type="text/css">
body{background-color:#494042;width:100%;margin-bottom:20px;min-width:600px;max-width:2000px;height:100%;}
body{background-color:#494042;width:100%;margin-bottom:20px;min-width:600px;max-width:2000px;height:100%;}
Line 114: Line 229:
top:141px;
top:141px;
width:100%;
width:100%;
 +
z-index:1;
}
}
ul, ol {
ul, ol {
Line 119: Line 235:
font-size: 14px;
font-size: 14px;
}
}
-
#slide{width:96%;height:auto;background-color:#F4DFC0;margin-top:80px;border:2px solid;
+
#slide{width:96%;margin-left:15px;height:auto;background-color:#FFFFff;margin-top:80px;border:2px solid;
-
border-radius:15px;padding-right:10px;margin-left:15px;}
+
border-radius:15px;padding-right:10px;}
.center
.center
{
{
Line 127: Line 243:
background-color:#b0e0e6;
background-color:#b0e0e6;
}
}
-
#interview,#presentation,#article, #interview1, #presentation1,#article1
+
#interview,#presentation, #article, #article2
-
{margin-top:20px;width:30%;margin-right:20px;float:left;height:250px; border:2px solid;
+
{width:35%;height:auto; border:2px solid;
-
border-radius:15px;font-family:"Trebuchet MS", Helvetica, sans-serif;}
+
border-radius:15px;padding:10px;margin-left:20px;display:none;background-color:#35BB91;font-family:"Trebuchet MS", Helvetica, sans-serif;}
-
#interview
+
#presentation
-
{margin-left:25px;background:#e32e51;}
+
{position:absolute;top:625px;left:610px;}
-
#interview1
+
#article
-
{margin-left:25px;background:#35bb91;}
+
{position:absolute;top:490px;left:610px;}
-
#presentation,#article1
+
#article2
-
{background:#35bb91;}
+
{position:absolute;top:380px;left:610px;}
-
#article,#presentation1
+
#projinteract{margin-top:10px;width:60%;float:left;height:400px; border:2px solid;
-
{background:#e32e51;}
+
border-radius:15px;padding:5px;background:#ffffff;margin-left:15px;}
-
#art-desc,#pre-desc,#int-desc{width:50%;float:right;margin-right:8px;}
+
#moddesc{margin-top:10px;width:96%;float:left;height:auto; border:2px solid;padding:10px;background:#ffffff;margin-left:20px;border-radius:15px;font-family:"Trebuchet MS", Helvetica, sans-serif;}
-
#art-img,#pre-img,#int-img{width:45%;float:left;margin-left:5px;}
+
#art-desc,#pre-desc,#int-desc{width:45%;float:right;margin-right:8px;}
 +
#art-img,#pre-img,#int-img{width:45%;float:left;}
#art-desc-text,#pre-desc-text,#int-desc-text{text-align:justify;float:right;}
#art-desc-text,#pre-desc-text,#int-desc-text{text-align:justify;float:right;}
-
h3,h1,#yo{margin-left:10px;font-family:"Trebuchet MS", Helvetica, sans-serif;}
+
h3{margin-left:10px;font-family:"Trebuchet MS", Helvetica, sans-serif;}
-
h1{font-size:35px;}
+
#isi{margin-left:10px;font-family:"Trebuchet MS", Helvetica, sans-serif;margin-top:10px;}
 +
h3.title{font-size:28px;color:#35BB91;}
 +
h3.kotak{margin-left:0px;}
 +
#iGEM_Logo{
 +
width:100px;
 +
height:80px;
 +
position:absolute;
 +
right:10px;
 +
top:60px;
 +
z-index:+15;
 +
}
 +
#hkust_Logo{
 +
width:60px;
 +
height:80px;
 +
position:absolute;
 +
right:110px;
 +
top:60px;
 +
z-index:+15;
 +
}
</style>
</style>
</head>
</head>
<body>
<body>
 +
 
 +
          <a href="https://2013.igem.org/Main_Page"><img id="iGEM_Logo" src="https://static.igem.org/mediawiki/2013/4/46/Igem_qgem_logo.png"></a>
 +
         
 +
 +
<a href="http://www.ust.hk/eng/index.htm"><img id="hkust_Logo" src="https://static.igem.org/mediawiki/2013/5/55/Hkust_logo.gif"></a>
 +
<a href=https://2013.igem.org/Team:Hong_Kong_HKUST><center><div id="kepala"><img src="https://static.igem.org/mediawiki/igem.org/c/c7/BANNER1_%281%29.png" style="height:121px;width:100%;"></div></center></a>
<a href=https://2013.igem.org/Team:Hong_Kong_HKUST><center><div id="kepala"><img src="https://static.igem.org/mediawiki/igem.org/c/c7/BANNER1_%281%29.png" style="height:121px;width:100%;"></div></center></a>
Line 168: Line 309:
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/abstract">Abstract</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/abstract">Abstract</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/modules">Modules Description</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/modules">Modules Description</a></li>
 +
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/data">Data Page</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Parts">Parts</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Parts">Parts</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/characterization">Characterization</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/characterization">Characterization</a></li>
-
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/modelling">Modeling</a></li>
 
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/results">Result</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/results">Result</a></li>
 +
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/future">Future Work</a></li>
</ul>
</ul>
</li>
</li>
Line 180: Line 322:
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/protocols">Protocols</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/protocols">Protocols</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/safety">Safety</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/safety">Safety</a></li>
-
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/future">Future Work</a></li>
+
 
</ul>
</ul>
</li>
</li>
Line 193: Line 335:
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp">Human Practice</a>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp">Human Practice</a>
<ul>
<ul>
-
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/interview">Interviews</a></li>
 
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/cp">Country Profile</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/cp">Country Profile</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/blog">Blog</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/blog">Blog</a></li>
-
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/article">Article</a></li>
+
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/interview">Interviews</a></li>
 +
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/article/genet">Article</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/video">Videos</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/video">Videos</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/presentation">Presentations</a></li>
<li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/presentation">Presentations</a></li>
Line 206: Line 348:
</div>
</div>
 +
<br><br><br><div id="slide"><h3 class="title">Project Outline</h3><p id="isi">Our ultimate goal is to build a ‘smart’ glyoxylate shunt that burns extra fatty acid when there is energy surplus. To achieve this, we would have to first build the shunt itself in mammalian cells. The shunt comprises two bacterial glyoxylate enzymes, isocitrate lyase (AceA) and malate synthase (AceB). Tagged with a localization signal peptide, they could be directed to the mitochondria and act on the citric acid cycle, eventually increasing fatty acid uptake. The two enzymes would be initially driven by constitutive promoters (CMV and EF-1alpha promoters), placing the shunt in a constantly “ON” state and burning calories regardless of the energy profile. To improve this, we are developing fatty acid responsive promoters, which, when used to regulate the glyoxylate enzymes, should dispense energy only when it is in excess. <strong>Hover</strong> your mouse and <strong>click</strong> on the images below to learn more about each modules!</p></div>
 +
 +
 +
 +
<div id="Table_01">
 +
<div id="cell-small-01">
 +
<img src="https://static.igem.org/mediawiki/2013/2/27/Cell-small_01.jpg" width="600" height="128" alt="">
 +
</div>
 +
<div id="cell-small-02">
 +
<img src="https://static.igem.org/mediawiki/2013/e/e7/Cell-small_02.jpg" width="121" height="272" alt="">
 +
</div>
 +
<div id="cell-small-03"> <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Project/module2"><img src="https://static.igem.org/mediawiki/2013/4/4e/Cell-small_03.jpg" width="95" height="84" alt=""></a>
 +
</div>
 +
<div id="cell-small-04">
 +
<img src="https://static.igem.org/mediawiki/2013/d/d0/Cell-small_04.jpg" width="117" height="84" alt="">
 +
</div>
 +
<div id="cell-small-05">
 +
<img src="https://static.igem.org/mediawiki/2013/9/94/Cell-small_05.jpg" width="70" height="159" alt="">
 +
</div>
 +
<div id="cell-small-06"> <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Project/module3"><img src="https://static.igem.org/mediawiki/2013/6/68/Cell-small_06.jpg" width="122" height="159" alt=""></a>
 +
</div>
 +
<div id="cell-small-07">
 +
<img src="https://static.igem.org/mediawiki/2013/b/be/Cell-small_07.jpg" width="75" height="272" alt="">
 +
</div>
 +
<div id="cell-small-08">
 +
<img src="https://static.igem.org/mediawiki/2013/2/26/Cell-small_08.jpg" width="120" height="75" alt="">
 +
</div>
 +
<div id="cell-small-09"> <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Project/module4"><img src="https://static.igem.org/mediawiki/2013/d/d3/Cell-small_09.jpg" width="92" height="75" alt=""></a>
 +
</div>
 +
<div id="cell-small-10">
 +
<img src="https://static.igem.org/mediawiki/2013/9/9f/Cell-small_10.jpg" width="404" height="113" alt="">
 +
</div>
 +
</div>
 +
 +
 +
<div id="projinteract"></div>
 +
<div id="article2" class="info1"><h3 class="kotak">Glyoxylate Shunt</h3><p><b><i>Responsible for:</b></i><br>Introduce glyoxylate enzymes responsible for the shunt<br><b><i>Parts submitted:</b></i><a href="http://parts.igem.org/Part:BBa_K1119002">BBa_K1119002</a>, <a href="http://parts.igem.org/Part:BBa_K1119003">BBa_K1119003</a>, <a href="http://parts.igem.org/Part:BBa_K1119006">BBa_K1119006</a>, <a href="http://parts.igem.org/Part:BBa_K1119008">BBa_K1119008</a>, <a href="http://parts.igem.org/Part:BBa_K1119010">BBa_K1119010</a> & <a href="http://parts.igem.org/Part:BBa_K1119006">BBa_K1119011</a></p></div>
 +
<div id="article" class="info1"><h3 class="kotak">Protein Trafficking</h3><p><b><i>Responsible for:</b></i><br>Target ACE proteins into mitochondria<br><b><i>Parts submitted:</b></i> <a href="http://parts.igem.org/Part:BBa_K1119000">BBa_K1119000</a>, <a href="http://parts.igem.org/Part:BBa_K1119001">BBa_K1119001</a> & <a href="http://parts.igem.org/Part:BBa_K1119009">BBa_K1119009</a></p></div>
 +
<div id="presentation" class="info1"><h3 class="kotak">Fatty Acid Sensing Mechanism</h3><p><b><i>Responsible for:</b></i><br>Introduce inducible system that allows tunable fatty acid uptake by sensing fatty acid concentration<br><b><i>Parts submitted:</b> - </i></p></div>
</body>
</body>
</html>
</html>

Latest revision as of 12:05, 28 October 2013




Project Outline

Our ultimate goal is to build a ‘smart’ glyoxylate shunt that burns extra fatty acid when there is energy surplus. To achieve this, we would have to first build the shunt itself in mammalian cells. The shunt comprises two bacterial glyoxylate enzymes, isocitrate lyase (AceA) and malate synthase (AceB). Tagged with a localization signal peptide, they could be directed to the mitochondria and act on the citric acid cycle, eventually increasing fatty acid uptake. The two enzymes would be initially driven by constitutive promoters (CMV and EF-1alpha promoters), placing the shunt in a constantly “ON” state and burning calories regardless of the energy profile. To improve this, we are developing fatty acid responsive promoters, which, when used to regulate the glyoxylate enzymes, should dispense energy only when it is in excess. Hover your mouse and click on the images below to learn more about each modules!

Glyoxylate Shunt

Responsible for:
Introduce glyoxylate enzymes responsible for the shunt
Parts submitted:BBa_K1119002, BBa_K1119003, BBa_K1119006, BBa_K1119008, BBa_K1119010 & BBa_K1119011

Protein Trafficking

Responsible for:
Target ACE proteins into mitochondria
Parts submitted: BBa_K1119000, BBa_K1119001 & BBa_K1119009

Fatty Acid Sensing Mechanism

Responsible for:
Introduce inducible system that allows tunable fatty acid uptake by sensing fatty acid concentration
Parts submitted: -

Retrieved from "http://2013.igem.org/Team:Hong_Kong_HKUST/modules"