Team:KIT-Kyoto/Notebook/ATF1/july

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<ul class="nav metro-nav-list">
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{{Template:KIT-Kyoto/menu_note}}
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  <li class="nav-header">LabNote</li>
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  <li class="">
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      <a href="#ATF1">ATF1</a>
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      <ul class="nav">
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          <li><a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/ATF1/may">May</a></li>
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          <li><a href="">june</a></li>
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          <li><a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/ATF1/july">july</a></li>
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          <li><a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/ATF1/august">August</a></li>
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          <li><a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/ATF1/september">September</a></li>
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      <a href="#https://2013.igem.org/Team:KIT-Kyoto/Notebook/ATF2">ATF2</a>
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      <a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/YJL">YJL068C</a>
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      <a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/growcurves">Growth Curves</a>
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<h2>ATF1</h2>
<h2>ATF1</h2>
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=4><B>July
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<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>July 1<sup>st</sup></span></b></p>
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1</B></FONT><SUP><FONT SIZE=4><B>st</B></FONT></SUP></SPAN></FONT></P>
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<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
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performed PCR at 53℃ and 54℃.</FONT></SPAN></FONT></P>
+
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<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
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applied DNA sample to the agarose gel electrophoresis.</FONT></SPAN></FONT></P>
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<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">-53℃
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ver-</FONT></SPAN></FONT></P>
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<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
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detected several bands.</FONT></SPAN></FONT></P>
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<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">-54℃
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ver-</FONT></SPAN></FONT></P>
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<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">The
+
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bands seems wrong size.</FONT></SPAN></FONT></P>
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<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1.
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We applied DNA sample to the blue gel electrophoresis and purified
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DNA.</FONT></SPAN></FONT></P>
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<P STYLE="margin-bottom: 0cm"> <FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">[Consequence]</FONT></SPAN></FONT></P>
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<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">The
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band appeared 1600bp.</FONT></SPAN></FONT></P>
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<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2.
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We performed PCR at 51℃ and 52℃.</FONT></SPAN></FONT></P>
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<TABLE WIDTH=189 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
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<TR VALIGN=TOP>
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<TD WIDTH=102>
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<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Buffer</FONT></SPAN></FONT></P>
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</TD>
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<TD WIDTH=56>
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<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">50&mu;l</FONT></SPAN></FONT></P>
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</TD>
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</TR>
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<TR VALIGN=TOP>
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<TD WIDTH=102>
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<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">dNTP</FONT></SPAN></FONT></P>
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</TD>
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<TD WIDTH=56>
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<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">20&mu;l</FONT></SPAN></FONT></P>
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</TD>
+
-
</TR>
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<TR VALIGN=TOP>
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<TD WIDTH=102>
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<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">primer
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mix</FONT></SPAN></FONT></P>
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</TD>
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<TD WIDTH=56>
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<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1&mu;l</FONT></SPAN></FONT></P>
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</TD>
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</TR>
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<TR VALIGN=TOP>
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<TD WIDTH=102>
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<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">PCR
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product</FONT></SPAN></FONT></P>
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</TD>
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<TD WIDTH=56>
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<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">4&mu;l</FONT></SPAN></FONT></P>
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-
</TD>
+
-
</TR>
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<TR VALIGN=TOP>
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<TD WIDTH=102>
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<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">KOD-FX</FONT></SPAN></FONT></P>
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</TD>
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<TD WIDTH=56>
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<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2&mu;l</FONT></SPAN></FONT></P>
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-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
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-
<TD WIDTH=102>
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<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">H2O</FONT></SPAN></FONT></P>
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</TD>
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<TD WIDTH=56>
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<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">23&mu;l</FONT></SPAN></FONT></P>
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</TD>
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-
</TR>
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</TABLE>
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<P STYLE="margin-bottom: 0cm"><BR>
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</P>
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<P STYLE="margin-bottom: 0cm"><BR>
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</P>
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<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=4><B>July
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2</B></FONT><SUP><FONT SIZE=4><B>nd</B></FONT></SUP></SPAN></FONT></P>
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<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
applied DNA sample to the agarose gel electrophoresis.</FONT></SPAN></FONT></P>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">[Consequence]</FONT></SPAN></FONT></P>
+
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<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">The
+
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band appear the correct place.</FONT></SPAN></FONT></P>
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-
<P STYLE="margin-bottom: 0cm"><BR>
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</P>
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-
<P STYLE="margin-bottom: 0cm"><BR>
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-
</P>
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<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=4><B>July
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4</B></FONT><SUP><FONT SIZE=4><B>th</B></FONT></SUP></SPAN></FONT></P>
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<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
purified DNA sample that PCR product in July 1</FONT><SUP><FONT SIZE=2 STYLE="font-size: 11pt">st</FONT></SUP><FONT SIZE=2 STYLE="font-size: 11pt">.</FONT></SPAN></FONT></P>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
digested </FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">
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with HindⅢ.</FONT></SPAN></FONT></P>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
applied DNA sample to the agarose gel electrophoresis.</FONT></SPAN></FONT></P>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">[Consequence]</FONT></SPAN></FONT></P>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">The
+
-
band appear correct place.</FONT></SPAN></FONT></P>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
performed PCR for ATF1.</FONT></SPAN></FONT></P>
+
-
<TABLE WIDTH=186 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
+
-
<TR VALIGN=TOP>
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-
<TD WIDTH=100>
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<P><FONT FACE="Century, serif"><SPAN LANG="en-US">Buffer</SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=56>
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-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US">50&mu;l</SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=100>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US">dNTP</SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=56>
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-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US">20&mu;l</SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
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-
<TD WIDTH=100>
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<P><FONT FACE="Century, serif"><SPAN LANG="en-US">primer mix</SPAN></FONT></P>
+
-
</TD>
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-
<TD WIDTH=56>
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<P><FONT FACE="Century, serif"><SPAN LANG="en-US">1&mu;l</SPAN></FONT></P>
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-
</TD>
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-
</TR>
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-
<TR VALIGN=TOP>
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<TD WIDTH=100>
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<P><FONT FACE="Century, serif"><SPAN LANG="en-US">PCR product</SPAN></FONT></P>
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-
</TD>
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-
<TD WIDTH=56>
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<P><FONT FACE="Century, serif"><SPAN LANG="en-US">4&mu;l</SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=100>
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<P><FONT FACE="Century, serif"><SPAN LANG="en-US">KOD-FX</SPAN></FONT></P>
+
-
</TD>
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-
<TD WIDTH=56>
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<P><FONT FACE="Century, serif"><SPAN LANG="en-US">2&mu;l</SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
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-
<TD WIDTH=100>
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<P><FONT FACE="Century, serif"><SPAN LANG="en-US">H2O</SPAN></FONT></P>
+
-
</TD>
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-
<TD WIDTH=56>
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<P><FONT FACE="Century, serif"><SPAN LANG="en-US">23&mu;l</SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
</TABLE>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=4><B>July5</B></FONT><SUP><FONT SIZE=4><B>th</B></FONT></SUP></SPAN></FONT><FONT SIZE=4><B>・</B></FONT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=4><B>8</B></FONT><SUP><FONT SIZE=4><B>th</B></FONT></SUP></SPAN></FONT></P>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
purified ATF1.</FONT></SPAN></FONT></P>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
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digested </FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1 </I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">and
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pET-15b with Xho1.</FONT></SPAN></FONT></P>
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<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
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purified this ATF1 and pET-15b.</FONT></SPAN></FONT></P>
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<P STYLE="margin-bottom: 0cm"><BR>
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</P>
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<P STYLE="margin-bottom: 0cm"><BR>
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</P>
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<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=4><B>July
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9</B></FONT><SUP><FONT SIZE=4><B>th</B></FONT></SUP></SPAN></FONT></P>
+
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<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
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digested </FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1 </I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">and
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pET-15b with  Bpu11021I.</FONT></SPAN></FONT></P>
+
-
<TABLE WIDTH=190 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
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<TR VALIGN=TOP>
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<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We performed PCR
-
<TD WIDTH=99>
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at 53</span><span style='font-size:11.0pt;font-family:"MS 明朝","serif"'>&#730;</span><span
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<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">ATF1</FONT></SPAN></FONT></P>
+
lang=EN-US style='font-size:11.0pt'>C and 54</span><span style='font-size:11.0pt;
-
</TD>
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font-family:"MS 明朝","serif"'>&#730;</span><span lang=EN-US style='font-size:11.0pt'>C.
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<TD WIDTH=61>
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(annealing temp.)</span></p>
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<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">25&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=99>
+
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<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Buffer</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=61>
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<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">3&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=99>
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-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Bpu1102
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I</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=61>
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<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=99>
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-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">total</FONT></SPAN></FONT></P>
+
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</TD>
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<TD WIDTH=61>
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<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">30&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
</TABLE>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><BR>
+
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</P>
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<TABLE WIDTH=189 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
+
-
<TR VALIGN=TOP>
+
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We applied DNA
-
<TD WIDTH=101>
+
sample to the agarose gel electrophoresis.</span></p>
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<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">pET-15b</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=59>
+
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<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">25&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=101>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Buffer</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=59>
+
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<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">3&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=101>
+
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<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Bpu1102
+
-
I</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=59>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=101>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">total</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=59>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">30&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
</TABLE>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
incubated at 37℃(30min)</FONT></SPAN></FONT></P>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
add 1uL BAP to </FONT></SPAN></FONT>
+
-
</P>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
incubated at 37℃(30min)</FONT></SPAN></FONT></P>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
applied DNA sample to the blue gel electrophoresis.</FONT></SPAN></FONT></P>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">[Consequence]</FONT></SPAN></FONT></P>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">The
+
-
band did not appear.</FONT></SPAN></FONT></P>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
performed PCR DNA sample that PCR product in July 1</FONT><SUP><FONT SIZE=2 STYLE="font-size: 11pt">st</FONT></SUP></SPAN></FONT></P>
+
-
<P STYLE="margin-left: 0.74cm; margin-bottom: 0cm"><BR>
+
-
</P>
+
-
<P STYLE="margin-left: 0.74cm; margin-bottom: 0cm"><BR>
+
-
</P>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm; widows: 2; orphans: 2"><BR>
+
-
</P>
+
-
<P STYLE="margin-bottom: 0cm; page-break-before: always"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=4><B>July
+
-
11th</B></FONT></SPAN></FONT></P>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
purified </FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1</I></FONT></SPAN></FONT></P>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
digested </FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1 </I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">with
+
-
Xho1.</FONT></SPAN></FONT></P>
+
-
<TABLE WIDTH=191 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
+
-
<TR VALIGN=TOP>
+
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>-53</span><span
-
<TD WIDTH=103>
+
style='font-size:11.0pt;font-family:"MS 明朝","serif"'>&#730;</span><span lang=EN-US
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">ATF1</FONT></SPAN></FONT></P>
+
style='font-size:11.0pt'>C ver.-</span></p>
-
</TD>
+
-
<TD WIDTH=58>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">25</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=103>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Xho1</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=58>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=103>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Buffer(10&times;H)</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=58>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">3</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=103>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">total</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=58>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">30</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
</TABLE>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><BR>
+
-
</P>
+
-
<TABLE WIDTH=191 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
+
-
<TR VALIGN=TOP>
+
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We detected several
-
<TD WIDTH=106>
+
bands.</span></p>
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">pET-15b</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=54>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">88</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=106>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Xho1</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=54>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=106>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Buffer(10&times;H)</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=54>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">10</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=106>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">total</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=54>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">100</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
</TABLE>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><BR>
+
-
</P>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
applied DNA sample to the agarose gel electrophoresis.</FONT></SPAN></FONT></P>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
purified DNA sample</FONT></SPAN></FONT></P>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
digested </FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I>ATF1 </I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">and
+
-
Vector</FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I> </I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">with
+
-
Bpu11021.</FONT></SPAN></FONT></P>
+
-
<TABLE WIDTH=191 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
+
-
<TR VALIGN=TOP>
+
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>-54</span><span
-
<TD WIDTH=101>
+
style='font-size:11.0pt;font-family:"MS 明朝","serif"'>&#730;</span><span lang=EN-US
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">ATF1</FONT></SPAN></FONT></P>
+
style='font-size:11.0pt'>C ver.-</span></p>
-
</TD>
+
-
<TD WIDTH=59>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">25&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=101>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Buffer</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=59>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">3&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=101>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Bpu1102
+
-
I</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=59>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=101>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">total</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=59>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">30&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
</TABLE>
+
-
<P ALIGN=LEFT STYLE="margin-left: 0.74cm; margin-bottom: 0cm"><BR>
+
-
</P>
+
-
<TABLE WIDTH=186 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
+
-
<TR VALIGN=TOP>
+
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The bands seems
-
<TD WIDTH=99>
+
wrong size.</span></p>
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">pET-15b</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=56>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">34&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=99>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Buffer</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=56>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=99>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Bpu1102
+
-
I</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=56>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">4&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=99>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">total</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=56>
+
-
<P ALIGN=LEFT><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">40&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
</TABLE>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
applied DNA sample to the blue gel electrophoresis.</FONT></SPAN></FONT></P>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">[result]</FONT></SPAN></FONT></P>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Failed</FONT></SPAN></FONT></P>
+
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm"><BR>
+
-
</P>
+
-
<P STYLE="margin-bottom: 0cm"><BR>
+
-
</P>
+
-
<P STYLE="margin-bottom: 0cm"><BR>
+
-
</P>
+
-
<P STYLE="margin-bottom: 0cm"><BR>
+
-
</P>
+
-
<P STYLE="margin-bottom: 0cm"><BR>
+
-
</P>
+
-
<P STYLE="margin-bottom: 0cm"><BR>
+
-
</P>
+
-
<P STYLE="margin-bottom: 0cm"><BR>
+
-
</P>
+
-
<P STYLE="margin-bottom: 0cm"><BR>
+
-
</P>
+
-
<P STYLE="margin-bottom: 0cm"><BR>
+
-
</P>
+
-
<P STYLE="margin-bottom: 0cm"><BR>
+
-
</P>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=4><B>July
+
-
12</B></FONT><SUP><FONT SIZE=4><B>th</B></FONT></SUP></SPAN></FONT></P>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
performed PCR at 51℃ and 48℃</FONT></SPAN></FONT></P>
+
-
<TABLE WIDTH=167 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
+
-
<TR VALIGN=TOP>
+
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1. We applied DNA
-
<TD WIDTH=85>
+
sample to the blue gel electrophoresis and purified DNA.</span></p>
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Buffer</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=52>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">50</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=85>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">dNTP</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=52>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">20</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=85>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Primer
+
-
mix</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=52>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=85>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">genome</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=52>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">0.5</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=85>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">KOD-FX</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=52>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=85>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">H2O</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=52>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">26.5</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
</TABLE>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
applied DNA sample to the blue gel electrophoresis.</FONT></SPAN></FONT></P>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">[result]
+
-
Failed</FONT></SPAN></FONT></P>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
performed PCR at 51℃</FONT></SPAN></FONT></P>
+
-
<TABLE WIDTH=182 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
+
-
<TR VALIGN=TOP>
+
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;[Consequence]</span></p>
-
<TD WIDTH=102>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Buffer</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=50>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">50</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=102>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">dNTP</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=50>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">20</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=102>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Primer
+
-
mix</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=50>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=102>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">ATF1plasmid</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=50>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">0.5</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=102>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">KOD-FX</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=50>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=102>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">H2O</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=50>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">26.5</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
</TABLE>
+
-
<P STYLE="margin-bottom: 0cm"><BR>
+
-
</P>
+
-
<P STYLE="margin-bottom: 0cm"><BR>
+
-
</P>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=4><B>July
+
-
17</B></FONT><SUP><FONT SIZE=4><B>th</B></FONT></SUP></SPAN></FONT></P>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
applied DNA sample to the agarose gel electrophoresis.</FONT></SPAN></FONT></P>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
performed PCR</FONT></SPAN></FONT></P>
+
-
<TABLE WIDTH=182 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
+
-
<TR VALIGN=TOP>
+
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The band appeared
-
<TD WIDTH=101>
+
1600bp.</span></p>
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Buffer</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=52>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">50</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=101>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">dNTP</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=52>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">20</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=101>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Primer
+
-
mix</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=52>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=101>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Genome
+
-
DNA</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=52>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">0.5</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=101>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">KOD-FX</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=52>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=101>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">H2O</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=52>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">26.5</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
</TABLE>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
extracted sample DNA for gel</FONT></SPAN></FONT></P>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
applied DNA sample to the agarose gel electrophoresis.</FONT></SPAN></FONT></P>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
performed PCR</FONT></SPAN></FONT></P>
+
-
<TABLE WIDTH=182 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
+
-
<TR VALIGN=TOP>
+
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2. We performed
-
<TD WIDTH=101>
+
PCR at 51</span><span style='font-size:11.0pt;font-family:"MS 明朝","serif"'>&#730;</span><span
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Buffer</FONT></SPAN></FONT></P>
+
lang=EN-US style='font-size:11.0pt'>C and 52</span><span style='font-size:11.0pt;
-
</TD>
+
font-family:"MS 明朝","serif"'>&#730;</span><span lang=EN-US style='font-size:11.0pt'>C.
-
<TD WIDTH=52>
+
(annealing temp.)</span></p>
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">50</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=101>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">dNTP</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=52>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">20</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=101>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Primer
+
-
mix</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=52>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=101>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Genome
+
-
DNA</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=52>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">0.5</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=101>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">KOD-FX</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=52>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">4</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=101>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">H2O</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=52>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">25</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
</TABLE>
+
-
<P STYLE="margin-bottom: 0cm"><BR>
+
-
</P>
+
-
<P STYLE="margin-bottom: 0cm"><BR>
+
-
</P>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=4><B>July
+
-
18</B></FONT><SUP><FONT SIZE=4><B>th</B></FONT></SUP></SPAN></FONT></P>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
extracted sample DNA for gel</FONT></SPAN></FONT></P>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
performed PCR.</FONT></SPAN></FONT></P>
+
-
<TABLE WIDTH=183 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
+
-
<TR VALIGN=TOP>
+
<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
-
<TD WIDTH=101>
+
style='border-collapse:collapse;border:none'>
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Buffer</FONT></SPAN></FONT></P>
+
<tr>
-
</TD>
+
  <td width=109 valign=top style='width:82.05pt;border:solid windowtext 1.0pt;
-
<TD WIDTH=52>
+
  padding:0mm 5.4pt 0mm 5.4pt'>
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">50</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-
</TD>
+
  style='font-size:11.0pt'>Buffer</span></p>
-
</TR>
+
  </td>
-
<TR VALIGN=TOP>
+
  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
-
<TD WIDTH=101>
+
  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">dNTP</FONT></SPAN></FONT></P>
+
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-
</TD>
+
  style='font-size:11.0pt'>50&micro;L</span></p>
-
<TD WIDTH=52>
+
  </td>
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">20</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
</tr>
-
</TD>
+
<tr>
-
</TR>
+
  <td width=109 valign=top style='width:82.05pt;border:solid windowtext 1.0pt;
-
<TR VALIGN=TOP>
+
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-
<TD WIDTH=101>
+
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Primer
+
  style='font-size:11.0pt'>dNTP</span></p>
-
mix</FONT></SPAN></FONT></P>
+
  </td>
-
</TD>
+
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
-
<TD WIDTH=52>
+
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">1&mu;l</FONT></SPAN></FONT></P>
+
  padding:0mm 5.4pt 0mm 5.4pt'>
-
</TD>
+
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-
</TR>
+
  style='font-size:11.0pt'>20&micro;L</span></p>
-
<TR VALIGN=TOP>
+
  </td>
-
<TD WIDTH=101>
+
</tr>
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">ATF1</FONT></SPAN></FONT></P>
+
<tr>
-
</TD>
+
  <td width=109 valign=top style='width:82.05pt;border:solid windowtext 1.0pt;
-
<TD WIDTH=52>
+
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-
</TD>
+
  style='font-size:11.0pt'>primer mix</span></p>
-
</TR>
+
  </td>
-
<TR VALIGN=TOP>
+
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
-
<TD WIDTH=101>
+
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">KOD-FX</FONT></SPAN></FONT></P>
+
  padding:0mm 5.4pt 0mm 5.4pt'>
-
</TD>
+
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-
<TD WIDTH=52>
+
  style='font-size:11.0pt'>1&micro;L</span></p>
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
  </td>
-
</TD>
+
</tr>
-
</TR>
+
<tr>
-
<TR VALIGN=TOP>
+
  <td width=109 valign=top style='width:82.05pt;border:solid windowtext 1.0pt;
-
<TD WIDTH=101>
+
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">H2O</FONT></SPAN></FONT></P>
+
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
-
</TD>
+
  lang=EN-US style='font-size:11.0pt'>PCR product</span></p>
-
<TD WIDTH=52>
+
  </td>
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">25</FONT><FONT FACE="Century, serif"><FONT SIZE=2 STYLE="font-size: 11pt">&mu;l</FONT></FONT></SPAN></FONT></P>
+
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
-
</TD>
+
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
-
</TR>
+
  padding:0mm 5.4pt 0mm 5.4pt'>
-
</TABLE>
+
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
  style='font-size:11.0pt'>4&micro;L</span></p>
-
applied DNA sample to the agarose gel electrophoresis.</FONT></SPAN></FONT></P>
+
  </td>
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
</tr>
-
performed PCR to use KOD-FXNeo and KOD-FX</FONT></SPAN></FONT></P>
+
<tr>
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
  <td width=109 valign=top style='width:82.05pt;border:solid windowtext 1.0pt;
-
applied DNA sample to the agarose gel electrophoresis.</FONT></SPAN></FONT></P>
+
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-
<P STYLE="margin-bottom: 0cm"><BR>
+
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-
</P>
+
  style='font-size:11.0pt'>KOD-FX</span></p>
-
<P STYLE="margin-bottom: 0cm"><BR>
+
  </td>
-
</P>
+
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm; widows: 2; orphans: 2"><BR>
+
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
-
</P>
+
  padding:0mm 5.4pt 0mm 5.4pt'>
-
<P ALIGN=LEFT STYLE="margin-bottom: 0cm; widows: 2; orphans: 2"><BR>
+
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-
</P>
+
  style='font-size:11.0pt'>2&micro;L</span></p>
-
<P STYLE="margin-bottom: 0cm; page-break-before: always"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=4><B>July
+
  </td>
-
19</B></FONT><SUP><FONT SIZE=4><B>th</B></FONT></SUP></SPAN></FONT></P>
+
</tr>
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
<tr>
-
purified PCR products.</FONT></SPAN></FONT></P>
+
  <td width=109 valign=top style='width:82.05pt;border:solid windowtext 1.0pt;
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-
digested</FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I> sample DNA
+
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-
</I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">with HindⅢ.</FONT></SPAN></FONT></P>
+
  style='font-size:11.0pt'>H<sub>2</sub>O</span></p>
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
  </td>
-
digested</FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I> </I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">pet-15b
+
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
-
and ATF1 with Xho1.</FONT></SPAN></FONT></P>
+
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
-
<TABLE WIDTH=173 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
+
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>23&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
</table>
-
<TR VALIGN=TOP>
+
<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
-
<TD WIDTH=103>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">ATF1</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=40>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">25&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=103>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Xho1</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=40>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=103>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Buffer(10&times;H)</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=40>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">3&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=103>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">total</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=40>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">30&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
</TABLE>
+
-
<P STYLE="margin-bottom: 0cm"><BR>
+
-
</P>
+
-
<TABLE WIDTH=173 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
+
-
<TR VALIGN=TOP>
+
<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
-
<TD WIDTH=103>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">pET-15b</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=40>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">34&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=103>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Xho1</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=40>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=103>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Buffer(10&times;H)</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=40>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">4&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=103>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">total</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=40>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">40&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
</TABLE>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
purified them.</FONT></SPAN></FONT></P>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
digested</FONT><FONT SIZE=2 STYLE="font-size: 11pt"><I> </I></FONT><FONT SIZE=2 STYLE="font-size: 11pt">pET-15b
+
-
and ATF1 with Bpu1102Ⅰ.</FONT></SPAN></FONT></P>
+
-
<TABLE WIDTH=174 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
+
-
<TR VALIGN=TOP>
+
<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>July 2<sup>nd</sup></span></b></p>
-
<TD WIDTH=103>
+
-
<P STYLE="margin-left: -0cm; margin-right: -0.83cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">pET-15b</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=41>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">25&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=103>
+
-
<P STYLE="margin-left: -0cm; margin-right: -0.85cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Buffer</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=41>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">3&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=103>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Bpu1102
+
-
I</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=41>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=103>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">total</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=41>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">30&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
</TABLE>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt"> </FONT></SPAN></FONT></P>
+
-
<TABLE WIDTH=174 BORDER=1 BORDERCOLOR="#00000a" CELLPADDING=7 CELLSPACING=0>
+
-
<TR VALIGN=TOP>
+
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
-
<TD WIDTH=104>
+
style='font-size:11.0pt'>We applied DNA sample to the agarose gel
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">ATF1</FONT></SPAN></FONT></P>
+
electrophoresis.</span></p>
-
</TD>
+
-
<TD WIDTH=41>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">25&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=104>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Buffer</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=41>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">3&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=104>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">Bpu1102
+
-
I</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=41>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">2&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
<TR VALIGN=TOP>
+
-
<TD WIDTH=104>
+
-
<P STYLE="margin-left: -0cm; margin-right: -0.44cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">total</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
<TD WIDTH=41>
+
-
<P><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">30&mu;l</FONT></SPAN></FONT></P>
+
-
</TD>
+
-
</TR>
+
-
</TABLE>
+
-
<P STYLE="margin-bottom: 0cm"><FONT FACE="Century, serif"><SPAN LANG="en-US"><FONT SIZE=2 STYLE="font-size: 11pt">We
+
-
applied DNA sample to the blue gel electrophoresis.</FONT></SPAN></FONT></P>
+
-
<P STYLE="margin-bottom: 0cm"><BR>
+
-
</P>
+
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>[Consequence]</span></p>
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>The band appeared at the correct size.</span></p>
 +
<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
 +
 +
<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>July 4<sup>th</sup></span></b></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We purified DNA
 +
sample (PCR product on July 1<sup>st</sup>.)</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We digested <i>ATF1</i>
 +
with <i>Hin</i>dIII.</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We applied DNA
 +
sample to the agarose gel electrophoresis.</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>[Consequence]</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The band appeared
 +
at the correct size.</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We performed PCR
 +
for <i>ATF1</i>.</span></p>
 +
 +
<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
 +
style='border-collapse:collapse;border:none'>
 +
<tr>
 +
  <td width=109 valign=top style='width:82.05pt;border:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US>Buffer</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
 +
  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US>50&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=109 valign=top style='width:82.05pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US>dNTP</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US>20&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=109 valign=top style='width:82.05pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US>primer mix</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US>1&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=109 valign=top style='width:82.05pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US>PCR
 +
  product</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US>4&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=109 valign=top style='width:82.05pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US>KOD-FX</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US>2&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=109 valign=top style='width:82.05pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US>H<sub>2</sub>O</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US>23&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
</table>
 +
 +
<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>July5<sup>th</sup></span></b><b><span
 +
style='font-size:14.0pt;font-family:"MS 明朝","serif"'>・</span></b><b><span
 +
lang=EN-US style='font-size:14.0pt'>8<sup>th</sup></span></b></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We purified <i>ATF1</i>.</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We digested <i>ATF1
 +
</i>and pET-15b with <i>Xho</i>I.</span></p>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>We purified this <i>ATF1</i> and pET-15b.</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
 +
 +
<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>July 9<sup>th</sup></span></b></p>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>We digested <i>ATF1 </i>and pET-15b with <i>Bpu</i>1102I.</span></p>
 +
 +
<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
 +
style='margin-left:42.0pt;border-collapse:collapse;border:none'>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>ATF1</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
 +
  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>Buffer</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><i><span
 +
  lang=EN-US style='font-size:11.0pt'>Bpu</span></i><span lang=EN-US
 +
  style='font-size:11.0pt'>1102I</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>total</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>30&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
</table>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>&nbsp;</span></p>
 +
 +
<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
 +
style='margin-left:42.0pt;border-collapse:collapse;border:none'>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>pET-15b</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
 +
  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>Buffer</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><i><span
 +
  lang=EN-US style='font-size:11.0pt'>Bpu</span></i><span lang=EN-US
 +
  style='font-size:11.0pt'>1102I</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>total</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>30&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
</table>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>We incubated at 37</span><span style='font-size:11.0pt;
 +
font-family:"MS 明朝","serif"'>&#730;</span><span lang=EN-US style='font-size:11.0pt'>C(30min)</span></p>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>We added 1uL BAP to vector.</span></p>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>We incubated at 37</span><span style='font-size:11.0pt;
 +
font-family:"MS 明朝","serif"'>&#730;</span><span lang=EN-US style='font-size:11.0pt'>C(30min)</span></p>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>We applied DNA sample to the blue gel electrophoresis.</span></p>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>[Consequence]</span></p>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>The band did not appear.</span></p>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>We performed PCR DNA sample (PCR product on July 1<sup>st</sup>.)</span></p>
 +
 +
<p class=MsoListParagraph style='margin-left:21.0pt'><span lang=EN-US
 +
style='font-size:11.0pt'>&nbsp;</span></p>
 +
 +
<p class=MsoListParagraph style='margin-left:21.0pt'><span lang=EN-US
 +
style='font-size:11.0pt'>&nbsp;</span></p>
 +
 +
<span lang=EN-US style='font-size:11.0pt;font-family:"Century","serif"'><br
 +
clear=all style='page-break-before:always'>
 +
</span>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>&nbsp;</span></p>
 +
 +
<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>July 11th</span></b></p>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>We purified <i>ATF1</i></span></p>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>We digested <i>ATF1 </i>with <i>Xho</i>I.</span></p>
 +
 +
<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
 +
style='margin-left:42.0pt;border-collapse:collapse;border:none'>
 +
<tr>
 +
  <td width=118 valign=top style='width:88.65pt;border:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>ATF1</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
 +
  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>25</span><span lang=EN-US
 +
  style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=118 valign=top style='width:88.65pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><i><span
 +
  lang=EN-US style='font-size:11.0pt'>Xho</span></i><span lang=EN-US
 +
  style='font-size:11.0pt'>I</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>2</span><span lang=EN-US
 +
  style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=118 valign=top style='width:88.65pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>Buffer(10xH)</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>3</span><span lang=EN-US
 +
  style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=118 valign=top style='width:88.65pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>Total</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>30</span><span lang=EN-US
 +
  style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
</table>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>&nbsp;</span></p>
 +
 +
<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
 +
style='margin-left:42.0pt;border-collapse:collapse;border:none'>
 +
<tr>
 +
  <td width=118 valign=top style='width:88.65pt;border:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>pET-15b</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
 +
  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>88</span><span lang=EN-US
 +
  style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=118 valign=top style='width:88.65pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><i><span
 +
  lang=EN-US style='font-size:11.0pt'>Xho</span></i><span lang=EN-US
 +
  style='font-size:11.0pt'>I</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>2</span><span lang=EN-US
 +
  style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=118 valign=top style='width:88.65pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>Buffer(10xH)</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>10</span><span lang=EN-US
 +
  style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=118 valign=top style='width:88.65pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>total</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>100</span><span lang=EN-US
 +
  style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
</table>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US>&nbsp;</span></p>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>We applied DNA sample to the agarose gel
 +
electrophoresis.</span></p>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>We purified DNA sample.</span></p>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>We digested <i>ATF1 </i>and Vector<i> </i>with <i>Bpu</i>1102I.</span></p>
 +
 +
<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
 +
style='margin-left:42.0pt;border-collapse:collapse;border:none'>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>ATF1</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
 +
  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>Buffer</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><i><span
 +
  lang=EN-US style='font-size:11.0pt'>Bpu</span></i><span lang=EN-US
 +
  style='font-size:11.0pt'>1102I</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>total</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>30&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
</table>
 +
 +
<p class=MsoListParagraph align=left style='margin-left:21.0pt;text-align:left'><span
 +
lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
 +
 +
<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
 +
style='margin-left:42.0pt;border-collapse:collapse;border:none'>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>pET-15b</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
 +
  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>34&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>Buffer</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><i><span
 +
  lang=EN-US style='font-size:11.0pt'>Bpu</span></i><span lang=EN-US
 +
  style='font-size:11.0pt'>1102I</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>4&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>total</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph align=left style='margin-left:0mm;text-align:left'><span
 +
  lang=EN-US style='font-size:11.0pt'>40&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
</table>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>We applied DNA sample to the blue gel electrophoresis.</span></p>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>[result]</span></p>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US
 +
style='font-size:11.0pt'>Failed</span></p>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US>&nbsp;</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
 +
 +
<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>&nbsp;</span></b></p>
 +
 +
<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>&nbsp;</span></b></p>
 +
 +
<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>&nbsp;</span></b></p>
 +
 +
<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>&nbsp;</span></b></p>
 +
 +
<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>&nbsp;</span></b></p>
 +
 +
<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>&nbsp;</span></b></p>
 +
 +
<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>&nbsp;</span></b></p>
 +
 +
<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>&nbsp;</span></b></p>
 +
 +
<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>July 12<sup>th</sup></span></b></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We performed PCR
 +
at 51</span><span style='font-size:11.0pt;font-family:"MS 明朝","serif"'>&#730;</span><span
 +
lang=EN-US style='font-size:11.0pt'>C and 48</span><span style='font-size:11.0pt;
 +
font-family:"MS 明朝","serif"'>&#730;</span><span lang=EN-US style='font-size:11.0pt'>C</span></p>
 +
 +
<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
 +
style='margin-left:42.0pt;border-collapse:collapse;border:none'>
 +
<tr>
 +
  <td width=100 valign=top style='width:75.25pt;border:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>Buffer</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border:solid windowtext 1.0pt;
 +
  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>50</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=100 valign=top style='width:75.25pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>dNTP</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>20</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=100 valign=top style='width:75.25pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>Primer mix</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>1&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=100 valign=top style='width:75.25pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>genome</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>0.5</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=100 valign=top style='width:75.25pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>KOD-FX</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>2</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=100 valign=top style='width:75.25pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>H<sub>2</sub>O</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>26.5</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
</table>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We applied DNA
 +
sample to the blue gel electrophoresis.</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>[result] Failed</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We performed PCR
 +
at 51</span><span style='font-size:11.0pt;font-family:"MS 明朝","serif"'>&#730;</span><span
 +
lang=EN-US style='font-size:11.0pt'>C</span></p>
 +
 +
<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
 +
style='margin-left:42.0pt;border-collapse:collapse;border:none'>
 +
<tr>
 +
  <td width=100 valign=top style='width:75.25pt;border:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>Buffer</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border:solid windowtext 1.0pt;
 +
  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>50</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=100 valign=top style='width:75.25pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>dNTP</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>20</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=100 valign=top style='width:75.25pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>Primer mix</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>1&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=100 valign=top style='width:75.25pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>ATF1plasmid</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>0.5</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=100 valign=top style='width:75.25pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>KOD-FX</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>2</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=100 valign=top style='width:75.25pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>H<sub>2</sub>O</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>26.5</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
</table>
 +
 +
<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
 +
 +
<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>July 17<sup>th</sup></span></b></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We applied DNA
 +
sample to the agarose gel electrophoresis.</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We performed PCR</span></p>
 +
 +
<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
 +
style='margin-left:42.0pt;border-collapse:collapse;border:none'>
 +
<tr>
 +
  <td width=116 valign=top style='width:86.7pt;border:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>Buffer</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border:solid windowtext 1.0pt;
 +
  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>50</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=116 valign=top style='width:86.7pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>dNTP</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>20</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=116 valign=top style='width:86.7pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>Primer mix</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>1&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=116 valign=top style='width:86.7pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>Genome DNA</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>0.5</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=116 valign=top style='width:86.7pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>KOD-FX</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>2</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=116 valign=top style='width:86.7pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>H<sub>2</sub>O</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>26.5</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
</table>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We extracted
 +
sample DNA for gel</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We applied DNA
 +
sample to the agarose gel electrophoresis.</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We performed PCR.</span></p>
 +
 +
<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
 +
style='margin-left:42.0pt;border-collapse:collapse;border:none'>
 +
<tr>
 +
  <td width=116 valign=top style='width:86.7pt;border:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>Buffer</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border:solid windowtext 1.0pt;
 +
  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>50</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=116 valign=top style='width:86.7pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>dNTP</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>20</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=116 valign=top style='width:86.7pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>Primer mix</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>1&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=116 valign=top style='width:86.7pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>Genome DNA</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>0.5</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=116 valign=top style='width:86.7pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>KOD-FX</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>4</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=116 valign=top style='width:86.7pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>H<sub>2</sub>O</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>25</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
</table>
 +
 +
<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
 +
 +
<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>July 18<sup>th</sup></span></b></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We extracted
 +
sample DNA for gel</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We performed PCR.</span></p>
 +
 +
<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
 +
style='margin-left:42.0pt;border-collapse:collapse;border:none'>
 +
<tr>
 +
  <td width=100 valign=top style='width:75.25pt;border:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>Buffer</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border:solid windowtext 1.0pt;
 +
  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>50</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=100 valign=top style='width:75.25pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>dNTP</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>20</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=100 valign=top style='width:75.25pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>Primer mix</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>1&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=100 valign=top style='width:75.25pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>ATF1</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>2</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=100 valign=top style='width:75.25pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>KOD-FX</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>2</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=100 valign=top style='width:75.25pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>H<sub>2</sub>O</span></p>
 +
  </td>
 +
  <td width=67 valign=top style='width:50.0pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
 +
  style='font-size:11.0pt'>25</span><span lang=EN-US style='font-size:11.0pt'>&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
</table>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We applied DNA
 +
sample to the agarose gel electrophoresis.</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We performed PCR
 +
to use KOD-FXNeo and KOD-FX</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We applied DNA
 +
sample to the agarose gel electrophoresis.</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US>&nbsp;</span></p>
 +
 +
<span lang=EN-US style='font-size:10.5pt;font-family:"Century","serif"'><br
 +
clear=all style='page-break-before:always'>
 +
</span>
 +
 +
<p class=MsoNormal align=left style='text-align:left'><span lang=EN-US>&nbsp;</span></p>
 +
 +
<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>July 19<sup>th</sup></span></b></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We purified PCR
 +
products.</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We digested<i> </i>sample
 +
DNA<i> </i>with<i> Hind</i>III.</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We digested<i> </i>pet-15b
 +
and <i>ATF1</i> with <i>Xho</i>I.</span></p>
 +
 +
<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
 +
style='margin-left:42.0pt;border-collapse:collapse;border:none'>
 +
<tr>
 +
  <td width=118 valign=top style='width:88.65pt;border:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>ATF1</span></i></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
 +
  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=118 valign=top style='width:88.65pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
 +
  lang=EN-US style='font-size:11.0pt'>I</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=118 valign=top style='width:88.65pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Buffer(10xH)</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=118 valign=top style='width:88.65pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>total</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>30&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
</table>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
 +
 +
<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
 +
style='margin-left:42.0pt;border-collapse:collapse;border:none'>
 +
<tr>
 +
  <td width=118 valign=top style='width:88.65pt;border:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>pET-15b</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
 +
  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>34&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=118 valign=top style='width:88.65pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Xho</span></i><span
 +
  lang=EN-US style='font-size:11.0pt'>I</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=118 valign=top style='width:88.65pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Buffer(10xH)</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>4&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=118 valign=top style='width:88.65pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>total</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>40&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
</table>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We purified them.</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We digested<i> </i>pET-15b
 +
and <i>ATF1</i> with <i>Bpu</i>1102I.</span></p>
 +
 +
<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
 +
style='margin-left:42.0pt;border-collapse:collapse;border:none'>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>pET-15b</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
 +
  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Buffer</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bpu</span></i><span
 +
  lang=EN-US style='font-size:11.0pt'>1102I</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>total</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>30&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
</table>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></p>
 +
 +
<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
 +
style='margin-left:42.0pt;border-collapse:collapse;border:none'>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>ATF1</span></i></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border:solid windowtext 1.0pt;
 +
  border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Buffer</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bpu</span></i><span
 +
  lang=EN-US style='font-size:11.0pt'>1102I</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td width=93 valign=top style='width:69.8pt;border:solid windowtext 1.0pt;
 +
  border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>total</span></p>
 +
  </td>
 +
  <td width=55 valign=top style='width:41.2pt;border-top:none;border-left:none;
 +
  border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  padding:0mm 5.4pt 0mm 5.4pt'>
 +
  <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>30&micro;L</span></p>
 +
  </td>
 +
</tr>
 +
</table>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We applied DNA
 +
sample to the blue gel electrophoresis.</span></p>
 +
 +
<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
</div>
</div>
 +
</div>
</div>
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</html>
 

Latest revision as of 09:16, 27 September 2013




ATF1

July 1st

We performed PCR at 53˚C and 54˚C. (annealing temp.)

We applied DNA sample to the agarose gel electrophoresis.

-53˚C ver.-

We detected several bands.

-54˚C ver.-

The bands seems wrong size.

1. We applied DNA sample to the blue gel electrophoresis and purified DNA.

 [Consequence]

The band appeared 1600bp.

2. We performed PCR at 51˚C and 52˚C. (annealing temp.)

Buffer

50µL

dNTP

20µL

primer mix

1µL

PCR product

4µL

KOD-FX

2µL

H2O

23µL

 

 

July 2nd

We applied DNA sample to the agarose gel electrophoresis.

[Consequence]

The band appeared at the correct size.

 

 

July 4th

We purified DNA sample (PCR product on July 1st.)

We digested ATF1 with HindIII.

We applied DNA sample to the agarose gel electrophoresis.

[Consequence]

The band appeared at the correct size.

We performed PCR for ATF1.

Buffer

50µL

dNTP

20µL

primer mix

1µL

PCR product

4µL

KOD-FX

2µL

H2O

23µL

July5th8th

We purified ATF1.

We digested ATF1 and pET-15b with XhoI.

We purified this ATF1 and pET-15b.

 

 

July 9th

We digested ATF1 and pET-15b with Bpu1102I.

ATF1

25µL

Buffer

3µL

Bpu1102I

2µL

total

30µL

 

pET-15b

25µL

Buffer

3µL

Bpu1102I

2µL

total

30µL

We incubated at 37˚C(30min)

We added 1uL BAP to vector.

We incubated at 37˚C(30min)

We applied DNA sample to the blue gel electrophoresis.

[Consequence]

The band did not appear.

We performed PCR DNA sample (PCR product on July 1st.)

 

 


 

July 11th

We purified ATF1

We digested ATF1 with XhoI.

ATF1

25µL

XhoI

2µL

Buffer(10xH)

3µL

Total

30µL

 

pET-15b

88µL

XhoI

2µL

Buffer(10xH)

10µL

total

100µL

 

We applied DNA sample to the agarose gel electrophoresis.

We purified DNA sample.

We digested ATF1 and Vector with Bpu1102I.

ATF1

25µL

Buffer

3µL

Bpu1102I

2µL

total

30µL

 

pET-15b

34µL

Buffer

2µL

Bpu1102I

4µL

total

40µL

We applied DNA sample to the blue gel electrophoresis.

[result]

Failed

 

 

 

 

 

 

 

 

 

 

July 12th

We performed PCR at 51˚C and 48˚C

Buffer

50µL

dNTP

20µL

Primer mix

1µL

genome

0.5µL

KOD-FX

2µL

H2O

26.5µL

We applied DNA sample to the blue gel electrophoresis.

[result] Failed

We performed PCR at 51˚C

Buffer

50µL

dNTP

20µL

Primer mix

1µL

ATF1plasmid

0.5µL

KOD-FX

2µL

H2O

26.5µL

 

 

July 17th

We applied DNA sample to the agarose gel electrophoresis.

We performed PCR

Buffer

50µL

dNTP

20µL

Primer mix

1µL

Genome DNA

0.5µL

KOD-FX

2µL

H2O

26.5µL

We extracted sample DNA for gel

We applied DNA sample to the agarose gel electrophoresis.

We performed PCR.

Buffer

50µL

dNTP

20µL

Primer mix

1µL

Genome DNA

0.5µL

KOD-FX

4µL

H2O

25µL

 

 

July 18th

We extracted sample DNA for gel

We performed PCR.

Buffer

50µL

dNTP

20µL

Primer mix

1µL

ATF1

2µL

KOD-FX

2µL

H2O

25µL

We applied DNA sample to the agarose gel electrophoresis.

We performed PCR to use KOD-FXNeo and KOD-FX

We applied DNA sample to the agarose gel electrophoresis.

 

 

 


 

July 19th

We purified PCR products.

We digested sample DNA with HindIII.

We digested pet-15b and ATF1 with XhoI.

ATF1

25µL

XhoI

2µL

Buffer(10xH)

3µL

total

30µL

 

pET-15b

34µL

XhoI

2µL

Buffer(10xH)

4µL

total

40µL

We purified them.

We digested pET-15b and ATF1 with Bpu1102I.

pET-15b

25µL

Buffer

3µL

Bpu1102I

2µL

total

30µL

              

ATF1

25µL

Buffer

3µL

Bpu1102I

2µL

total

30µL

We applied DNA sample to the blue gel electrophoresis.