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Team:OU-Norman OK/Project/Notebook
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| - | <p class = "date"> | + | <p class = "date">01/16/13</p> |
| - | <p> | + | <h1 style="text-align:center">Making Agar Plates</h1> |
| + | <p style="text-align:center">LB agar is used to grow our stocks of Escherichia coli</p> | ||
| + | <p style="text-align:left">Recipe: Per 1000 mL</p> | ||
| + | <p style="text-align:left">10g Bacto Tryptone 10g</p> | ||
| + | <p style="text-align:left">5g Yeast Extract</p> | ||
| + | <p style="text-align:left">10g Sodium Chloride (NaCl)</p> | ||
| + | <p style="text-align:left">15g Agarose</p> | ||
| + | |||
| + | <p style="text-align:center">Mix Components in 1L of dH<sub>2</sub>O until dissolved. Spilt 1000mL solution into two flask. Cap flask with aluminum to prevent spilling of solution. Autoclave on slow exhaust for 20 minutes. Keep liquid in 65°C water bath to prevent setting until 18-Jan. </p> | ||
| + | |||
| + | <p class = "date">01/18/13</p> | ||
| + | <p style="text-align:center">Poured LB agar plates | ||
| + | |||
| + | <p class = "date">01/23/13</p> | ||
| + | <p style="text-align:center">Sealed TOP10 E. coli cells on LB agar plate. Stored in 37°C incubator</p> | ||
| + | |||
| + | <p class = "date">01/25/13</p> | ||
| + | <p style="text-align:center">Restreaked TOP10 cells on LB plates for isolation of single colonies.</p> | ||
| + | |||
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Revision as of 20:40, 7 August 2013
01/16/13
Making Agar Plates
LB agar is used to grow our stocks of Escherichia coli
Recipe: Per 1000 mL
10g Bacto Tryptone 10g
5g Yeast Extract
10g Sodium Chloride (NaCl)
15g Agarose
Mix Components in 1L of dH2O until dissolved. Spilt 1000mL solution into two flask. Cap flask with aluminum to prevent spilling of solution. Autoclave on slow exhaust for 20 minutes. Keep liquid in 65°C water bath to prevent setting until 18-Jan.
01/18/13
Poured LB agar plates
01/23/13
Sealed TOP10 E. coli cells on LB agar plate. Stored in 37°C incubator
01/25/13
Restreaked TOP10 cells on LB plates for isolation of single colonies.
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