Team:OU-Norman OK/Project/Notebook

From 2013.igem.org

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<h1></h1>
<p class = "date">01/16/13</p>
<p class = "date">01/16/13</p>
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<p style="text-align:center"><b>Making Agar Plates</b></p>  
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<p id = "notetitle">Making Agar Plates</p>  
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<p style="text-align:left">LB agar is used to grow our stocks of Escherichia coli</p>
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<p>LB agar is used to grow our stocks of Escherichia coli</p>
</br>
</br>
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<p style="text-align:left">Recipe: Per 1000 mL</p>
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<p>Recipe: Per 1000 mL</p>
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<p style="text-align:left">10g Bacto Tryptone 10g</p>
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<p>10g Bacto Tryptone 10g</p>
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<p style="text-align:left">5g Yeast Extract</p>
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<p>5g Yeast Extract</p>
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<p style="text-align:left">10g Sodium Chloride (NaCl)</p>
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<p>10g Sodium Chloride (NaCl)</p>
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<p style="text-align:left">15g Agarose</p>
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<p>15g Agarose</p>
</br>
</br>
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<p style="text-align:center">Mix Components in 1L of dH<sub>2</sub>O until dissolved. Spilt 1000mL solution into two flask. Cap flask with aluminum to prevent spilling of solution. Autoclave on slow exhaust for 20 minutes. Keep liquid in 65&#176;C water bath to prevent setting until 18-Jan. </p>
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<p>Mix Components in 1L of dH<sub>2</sub>O until dissolved. Spilt 1000mL solution into two flask. Cap flask with aluminum to prevent spilling of solution. Autoclave on slow exhaust for 20 minutes. Keep liquid in 65&#176;C water bath to prevent setting until 18-Jan. </p>
<p class = "date">01/18/13</p>
<p class = "date">01/18/13</p>
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<p style="text-align:left">Poured LB agar plates
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<p>Poured LB agar plates
<p class = "date">01/23/13</p>
<p class = "date">01/23/13</p>
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<p style="text-align:left">Sealed TOP10 E. coli cells on LB agar plate. Stored in 37&#176;C incubator</p>
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<p>Sealed TOP10 E. coli cells on LB agar plate. Stored in 37&#176;C incubator</p>
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<p class = "date">01/25/13</p>
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<p>01/25/13</p>
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<p style="text-align:left">Restreaked TOP10 cells on LB plates for isolation of single colonies.</p>
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<p>Restreaked TOP10 cells on LB plates for isolation of single colonies.</p>
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<h></h>
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<h1></h1>
<p class = "date">02/1/13</p>
<p class = "date">02/1/13</p>
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<p style="text-align:left">Antibiotics are used to isolate organisms into which plasmids containing antibiotic resistance genes have been transformed.</p>
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<p>Antibiotics are used to isolate organisms into which plasmids containing antibiotic resistance genes have been transformed.</p>
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<p style="text-align:left">Make antibiotic plates with the following specs.</p>
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<p>Make antibiotic plates with the following specs.</p>
</br>
</br>
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<table border="1">
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<table id = "notetable">
<tr>
<tr>
<th>Antibiotic</th>
<th>Antibiotic</th>

Revision as of 21:03, 7 August 2013

01/16/13

Making Agar Plates

LB agar is used to grow our stocks of Escherichia coli


Recipe: Per 1000 mL

10g Bacto Tryptone 10g

5g Yeast Extract

10g Sodium Chloride (NaCl)

15g Agarose


Mix Components in 1L of dH2O until dissolved. Spilt 1000mL solution into two flask. Cap flask with aluminum to prevent spilling of solution. Autoclave on slow exhaust for 20 minutes. Keep liquid in 65°C water bath to prevent setting until 18-Jan.

01/18/13

Poured LB agar plates

01/23/13

Sealed TOP10 E. coli cells on LB agar plate. Stored in 37°C incubator

01/25/13

Restreaked TOP10 cells on LB plates for isolation of single colonies.

02/1/13

Antibiotics are used to isolate organisms into which plasmids containing antibiotic resistance genes have been transformed.

Make antibiotic plates with the following specs.


Antibiotic Concentration (µ/mL)
row 1, cell 1 row 1, cell 2
row 2, cell 1 row 2, cell 2
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