01/16/13

Making Agar Plates

LB agar is used to grow our stocks of Escherichia coli

Recipe: Per 1000 mL

10g Bacto Tryptone 10g

5g Yeast Extract

10g Sodium Chloride (NaCl)

15g Agarose

Mix Components in 1L of dH₂O until dissolved. Spilt 1000mL solution into two flask. Cap flask with aluminum to prevent spilling of solution. Autoclave on slow exhaust for 20 minutes. Keep liquid in 65°C water bath to prevent setting until 18-Jan.

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01/18/13

Poured LB agar plates

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01/23/13

Sealed TOP10 E. coli cells on LB agar plate. Stored in 37°C incubator

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01/25/13

Restreaked TOP10 cells on LB plates for isolation of single colonies.

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02/1/13

Antibiotics are used to isolate organisms into which plasmids containing antibiotic resistance genes have been transformed.

Make antibiotic plates with the following specs.

Antibiotic	Concentration (µ/mL)
row 1, cell 1	row 1, cell 2
row 2, cell 1	row 2, cell 2

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