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- | <section id="Week7">
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- | <div class="page-header">
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- | <h1>Week7</h1>
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- | </div>
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- | <div class="row">
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- | <div class="span9">
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- | <div class="row">
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- | <h4>August 4th</h4>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Ligation: J23101 order vector with chloramphenicol resistance; double2 order
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- | RBS-GFPLVA- single2-terminator and vector with chloramphenicol resistance.<br/>2. Transform: transform the ligastion product into Top 10.<br/>3. Bacterial coating
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: Get BBa_K1059006 sequence from PCR product.<br/> 2. Agarose electrophoresis: detect the PCR product.<br/>3. Product purification: purify the PCR product.<br/>4. Ligation: recombine PCR product to pMD-19 T vector.<br/>5. Sequencing<br/>6. Transformation: transform the ligastion product into Top 10.
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. SDS page gel electrophoresis.
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- | </font></p></div>
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- | <h4>August 5th</h4>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Blue -white selection.<br/>2. PCR and electrophoresis examining.<br/>
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- | 3. PCR and electrophoresis examining, get the aim fragment.<br/>4. Sequencing.
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Culture the E.coli cells: pSB1C3.
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria: pick bacteria for PCR.<br/>2. PCR: use pcr to detect the bacteria we want.(double2-RBS-GFPLVA-single2-terminator-PSB1C3), 25ul system.<br/>3. Electrophoresis.<br/>4. Send sequencing.<br/>5. Conservation: conservate E.coli with.double2-RBS-GFPLVA-single2-terminator-PSB1C3 plasmid.
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- | </font></p></div>
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- | <h4>August 6th</h4>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Plasmid minprep.<br/>
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- | <img src="https://static.igem.org/mediawiki/2013/8/85/Ouc-week4.jpg" height="400" width="300" />
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Miniprep: pSB1C3.<br/>2. Digestion: digest the plasmid with XbaI and Pst-HF.<br/>3. SDS page gel electrophoresis.<br/>4. Gel extraction of DNA.<br/>5. PCR production purification: J23106+RBS, mamQ, mamL, B0015.<br/>6. SDS page gel electrophoresis: J23106+RBS, mamQ, mamL, B0015.<br/>
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- | <img src="https://static.igem.org/mediawiki/2013/9/99/Ouc-week5.png" height="400" width="300" /><br/>100bp, J23106+RBS, mamQ, mamL, B0015<br/>7. PCR amplification: J23106+RBS, B0015.<br/>8. SDS page gel electrophoresis: J23106+RBS, B0015.
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: Get mamJ sequence from PCR product and add prefix and suffix onto the sequence.<br/>2. PCR: Get mamQ sequence from PCR product and add prefix and suffix onto the sequence.<br/>3. Agarose electrophoresis: detect the PCR product.<br/>4. Product purification: purify the PCR product.<br/>5. Ligation: BBa_J23101-protection order 1-RBS-GFPLVA-terminator and vector with chloramphenicol resistance.<br/>
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- | <img src="https://static.igem.org/mediawiki/2013/f/f4/Ouc-week6.jpg" height="400" width="300" />
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Plasmid miniprep: plasmid Extraction of pSB1C3 -RFP for 5 tubes.<br/>2. Enzyme digestion: enzyme digestion of pSB1C3 for 50ul system of 3 tubes, use Xba1, Pst1.<br/>3. Electrophoresis: gel is good.<br/>
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- | <img src="https://static.igem.org/mediawiki/2013/c/c7/Ouc-week7.jpg" height="400" width="300" />
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- | </font></p></div>
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- | <h4>August 7th</h4>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterination.
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Transform: transform the ligastion product into Top 10.<br/>2. PCR: Get mamL sequence from PCR product and add prefix and suffix onto the sequence.<br/>3. PCR: Get mamQ sequence from PCR product and add prefix and suffix onto the sequence.<br/>4. Agarose electrophoresis: detect the PCR product.<br/>5. PCR: Get J23106+B0032 sequence from PCR product and add prefix and suffix onto the sequence.<br/>6. PCR: Get B0015 sequence from PCR product and add prefix and suffix onto the sequence.<br/>7. Agarose electrophoresis: detect the PCR product.<br/>8. Product purification: purify the PCR product.<br/>
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Gel recovery: gel recovery of pSB1C3.<br/>2. Electrophoresis: gel is good for 30ng/ul each.<br/>3. PCR: use PCR to B0015 cloning, 50ul system.<br/>4. Electrophoresis: gel is good. <br/>5. PCR Purification: PCR Purification of B0015.<br/>6. Electrophoresis: gel is good for 30ng/ul each.<br/>7. Enzyme digestion: enzyme digestion of RBS-mamL for 25ul system, use Xba1, Pst1;RBS-mamL for 25ul system, use Spe1; B0015 for 25ul system, use Xba1; promoter-RBS for 25ul system, use EcoR1, Pst1.<br/>8. Ligation: RBS-mamL order pSB1C3; promoter-RBS order pSB1C3; RBS-mamL order B0015.<br/>9. Transform: transform the ligastion product into Top 10.<br/>10. Bacterial coating.<br/>11. Bacterination: bacterinate E.coli with PSB1C3-RFP into tubes of 10ml.
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR amplification: mamL, three tubes.<br/>2. PCR production purification: mamL, three tubes; J23106+RBS, three tubes.<br/> 3. SDS page gel electrophoresis: mamL, three tubes; J23106+RBS, three tubes.<br/>
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- | <img src="https://static.igem.org/mediawiki/2013/1/1e/Ouc-week8.png" height="400" width="300" /><br/>J23106+RBS, mamL<br/>4. Digestion: digest RBS+ mamL with XbaI and Pst-HF, digest RBS+mamL with SpeI, digest B0015 with XbaI, digest J23106+RBS with EcoRI and Pst-HF.<br/>5. SDS page gel electrophoresis.<br/>6. Ligation: ligate RBS+mamL with Psb1c3, ligate J23106+RBS with pSB1C3,ligate RBS+ mamL with B0015.
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- | </font></p></div>
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- | <h4>August 8th</h4>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Miniprep the pSB1C3, J23101, RGL-K1059005.
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- | </font></p></div>
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- | <h4>August 9th</h4>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Enzyme digestion: BBa_J23101 with EcoR I and SpeI, BBa_ K1059002 with XbaI and PstI, BBa_K1059005 with EcoR I and PstI, pSB1C3 with EcoRI and PstI.<br/>2. Gel extraction pSB1C3 as vector.
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Miniprep: pSB1C3, ten tubes.<br/>2. Digestion: three tubes are digested with XbaI and Pst-HF, two tubes are digested with EcoRI and Pst-HF.<br/>3. SDS page gel electrophoresis.<br/>4. Gel extraction of DNA.<br/>5. PCR amplification: mamQ, four tubes. <br/>6. SDS page gel electrophoresis.<br/>7. PCR production purification: mamQ, two tubes.<br/>8. SDS page gel electrophoresis.<br/>9. Digestion: digest J23106+RBS with EcoRI and SpeI, digest RBS+mamQ with XbaI and Pst-HF.<br/>10. PCR amplification: RBS+mamL, four tubes.
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria: pick bacteria for PCR.<br/>2. PCR: use pcr to detect the bacteria we want(promoter-RBS-PSB1C3, RBS-mamL-PSB1C3), 25ul system.<br/>3. Enzyme digestion: enzyme digestion of PSB1C3 for 50ul system, 3 tubes, use Xba1, Pst1; pSB1C3 for 25ul system of 2 tubes, use EcoR1, Pst1.<br/>4. PCR: use pcr to cloning RBS-mamL-B0015, 50ul system.<br/>5.Electrophoresis: gel is good. <br/>6. Gel recovery: gel recovery of pSB1C3.<br/>7. Electrophoresis: gel is good for 20ng/ul each.<br/>
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- | <img src="https://static.igem.org/mediawiki/2013/d/d9/Ouc-week9.jpg" height="400" width="300" /><br/>Electrophoresis of PSB1C3 vector<br/>
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- | </font></p></div>
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- |
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- | <h4>August 10th</h4>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Recombination.
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. SDS page gel electrophoresis.<br/>2. PCR amplification: J23106+RBS, two tubes; B0015, two tubes. <br/>3. PCR production purification: RBS+mamL, four tubes.<br/>4. SDS page gel electrophoresis.<br/>5. Digestion: digest RBS+mamL with SpeI.<br/>6. Culture the E.coli cells: JB11.<br/>7. PCR production purification: mamI.<br/>8. SDS page gel electrophoresis.
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Product purification: purify the PCR product, J1.<br/>2. Plastid extraction: B0015, J2310, C+.<br/>3. Digestion: use enzyme XbaI, Pst I to get part B0015, a double terminator. <br/>4. Ligation: mamK onto pMB-19 T, prepare for sequence.<br/>5. PCR: amplification mamJ sequence from PCR product.<br/>6. Agarose electrophoresis: detect the PCR product.<br/>7. Get part for agrose, mamJ.<br/>8. PCR: Get mamI sequence from PCR product and add prefix and suffix onto the sequence.<br/>9. Agarose electrophoresis: detect the PCR product.<br/>10. Product purification: purify the PCR product.
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR Purification: PCR Purification of mamL1~4.<br/>2. Conservation: promoter-RBS-PSB1C3 (P4), RBS-mamL-PSB1C3 (L4).<br/>3. Ligation: RBS-mamJ, RBS-mamI, RBS-mamB order PMD-19 vector separately.
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- | </font></p></div>
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- | </div>
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- | </div>
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- | </div>
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- | <br /><br /></br>
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- | </section>
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| |
- | <section id="Week8">
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- | <div class="page-header">
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- | <h1>Week8</h1>
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- | </div>
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- | <div class="row">
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- |
| |
- | <div class="span9">
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- | <div class="row">
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- | <h4>August 11th</h4>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Transformation.<br/>2. Plate cultivation for 14h.
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: use PCR to detect mamQ1~10, 25ul system.<br/>2. Electrophoresis: gel is bad.<br/>3. Transform: transform the ligastion product ( RBS-mamJ-T vector;RBS-mamI-T vector, RBS-mamB-T vector, RBS-mamK-T vector) into Top 10.
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Miniprep: JB11, B0015, each two tubes.<br/>2. Digestion: digest JB11 with EcoRI and SpeI, digest B0015 with XbaI and Pst-HF, digest RBS+mamL with XbaI and SpeI.<br/>3. Ligation: ligate RBS+J23106 with pSB1C3, ligate RBS+mamL and BOO15 with pSB1C3.
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- | </font></p></div>
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- |
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- | <h4>August 12th</h4>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria and PCR examining
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Transform: transform the ligastion product(RBS-mamL-B0015-PSB1C3; promoter-RBS-PSB1C3) into Top 10.<br/>2. Bacterial coating.<br/>3. Pick bacteria: pick bacteria we want(RBS-mamL-B0015-PSB1C3, promoter-RBS-mamQ-PSB1C3).
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick the bacteria and culture the E.coli cells for four hours.<br/>2. PCR: the cultured E.coli cells.
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- | </font></p></div>
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- |
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- | <h4>August 13th</h4>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: the cultured E.coli cells.<br/>2. SDS page gel electrophoresis.<br/>3. Ligation: ligate the purified mamB and mamL with T vector.<br/>4. PCR amplification: mamB, mamK, mamJ.<br/>5. SDS page gel electrophoresis.
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Sequencing.
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- | </font></p></div>
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- | <h4>August 14th</h4>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR amplification: mamJ.<br/>2. SDS page gel electrophoresis.
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria: pick bacteria we want(RBS-mamL-B0015-PSB1C3, promoter-RBS-mamQ-PSB1C3).
| |
- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterination.<br/>2. Make competent cells.
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- | </font></p></div>
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- |
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- | <h4>August 15th</h4>
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- |
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: get mamJ sequence from AMB-1 bacterial strain.<br/>2. Agarose electrophoresis: detect the plasmid PCR product.<br/>3. Product purification: purify the PCR product.<br/>4. PCR: get mamC sequence from AMB-1 bacterial strain.
| |
- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Miniprep.<br/>2. Make gel.
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. SDS page gel electrophoresis.<br/>2. PCR amplification: mamL.<br/>3. PCR production purification: RBS+mamL.<br/>4. SDS page gel electrophoresis.
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- | </font></p></div>
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Ligastion: mamJ, mamK order PMD19-T vector.
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- | </font></p></div>
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- |
| |
- | <h4>August 16th</h4>
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- |
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- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
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- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Enzyme digestion.
| |
- | </font></p></div>
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- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: use PCR to detect mamK-pMD19-T vector1~15, 25ul system.
| |
- | </font></p></div>
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- |
| |
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| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR amplification: mamL.<br/>2. SDS page gel electrophoresis.
| |
- | </font></p></div>
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- |
| |
- | <h4>August 17th</h4>
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- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
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- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: amplification mamC sequence from PCR product.<br/>2. Agarose electrophoresis: detect the plasmid PCR product.<br/>3. Product purification: purify the PCR product.<br/>4. PCR: amplification mamI sequence from PCR product.<br/>5. PCR: amplification mamJ sequence from PCR product.<br/>6. Agarose electrophoresis: detect the plasmid PCR product.<br/>7. Product purification: purify the PCR product.<br/>8. PCR: amplification mamB sequence from PCR product.<br/>9. Agarose electrophoresis: detect the plasmid PCR product.<br/>10. Product purification: purify the PCR product.
| |
- | </font></p></div>
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| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. SDS page gel electrophoresis.<br/>2. PCR amplification: mamQ, two tubes.<br/>3. PCR production purification: RBS+mamL.<br/>4. SDS page gel electrophoresis.<br/>5. PCR amplification: mamK, two tubes; mamB, two tubes.<br/>6. SDS page gel electrophoresis.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Electrophoresis: mamK-PMD19-T vector1~15, gel is bad.<br/>2. Pick bacteria: pick bacteria we want (mamK-PMD19-T vector1~10).<br/>3. PCR: use PCR to detect mamK-pMD19-T vector1~10, 25ul system.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Recombination.
| |
- | </font></p></div>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </div>
| |
- | <br /><br /></br>
| |
- | </section>
| |
- |
| |
- | <section id="Week9">
| |
- | <div class="page-header">
| |
- | <h1>Week9</h1>
| |
- | </div>
| |
- | <div class="row">
| |
- |
| |
- | <div class="span9">
| |
- | <div class="row">
| |
- | <h4>August 18th</h4>
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Transformation.<br/>2. Plate cultivation for 14h.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Gel recovery: gel recovery of mamE.<br/>2. Electrophoresis: gel is good for 8ng/ul each.<br/>3. Bacterination: bacterination of E.coli with PSB1C3 plasmid for 3 tubes, 10ml each.<br/>4. Bacterial coating: JQ11, QP11, KP1, BP1, BP2, LP21.<br/>5. Gel recovery: gel recovery of PSB1C3 vector.<br/>6. Electrophoresis: gel is good for 20ng/ul each.<br/>
| |
- | <img src="https://static.igem.org/mediawiki/2013/0/06/Ouc-week10.jpg" height="400" width="300" /><br/>Electrophoresis of PSB1C3 vector<br/>
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR amplification: mamI, two tubes; mamQ, two tubes.<br/>2. SDS page gel electrophoresis.<br/>3. PCR production purification: RBS+mamQ, RBS+mamI.<br/>4. SDS page gel electrophoresis.<br/>5. PCR amplification: mamI, two tubes; mamJ, two tubes.<br/>6. SDS page gel electrophoresis.<br/>7. Transformation: JQ11, QP11, BP1, BP2, LP21, KP1.<br/>8. SDS page gel electrophoresis.<br/>9. PCR amplification: mamI, two tubes; mamB, two tubes.<br/>10. SDS page gel electrophoresis: mamK; mamB.<br/>
| |
- | <img src="https://static.igem.org/mediawiki/2013/6/64/Ouc-week11.png" height="400" width="300" /><br/>lane6 mamK1; lane7 mamK2; lane8 mamB<br/>
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Digestion: use enzyme EcoR I, Pst I to Cut PSB1C3 plasmid vector.<br/>2. Digestion: use enzyme EcoR I, Pst I to Cut promoter J23106 and RBS B0032. <br/>3. PCR: amplification mamE, mamJ sequence from PCR product.<br/>4. Agarose electrophoresis: detect the plasmid PCR product.<br/>5. Ligastion: promoter, RBS and mamQ.<br/>6. Ligastion: mamQ and PSB1C3.<br/>7. Ligastion: mamB and PSB1C3.<br/>8. Ligastion: mamL and PSB1C3.<br/>9. Ligastion: mamK and PSB1C3.<br/>10. Transform: transform the ligastion product into Top 10.
| |
- | </font></p></div>
| |
- |
| |
- | <h4>August 19th</h4>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria and PCR examining.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: use PCR to detect mamK-PSB1C3 vector1~10,25ul system.<br/>2. Electrophoresis: gel is bad.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Ligation: ligate the purified mamE with T vector.<br/>2. PCR production purification: RBS+mamB, two tubes.<br/>3. Pick the bacteria and culture the E.coli cells for four hours.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: amplification mamE sequence from PCR product.<br/>2. Agarose electrophoresis: detect the plasmid PCR product.<br/>3. PCR: amplification mamI sequence from PCR product.<br/>4. Product purification: purify the PCR product.<br/>5. Agarose electrophoresis: detect the PCR product purification.<br/>
| |
- | </font></p></div>
| |
- |
| |
- | <h4>August 20th</h4>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick the bacteria and culture the E.coli cells for four hours.<br/>2. PCR production purification: RBS+mamI, two tubes; RBS+mamB, one tube.<br/>3. SDS page gel electrophoresis.<br/>4. PCR amplification: mamI, four tubes.<br/>5. SDS page gel electrophoresis.<br/>6. Pick the bacteria and culture the E.coli cells for four hours.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Sequencing J23101.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: use PCR to detect promoter-RBS-mamQ-PSB1C3 vector1~10, 25ul system; mamK - PSB1C31~28, 25ul system; mamE-PMD19-T vector1~8, 25ul system.<br/>2. Electrophoresis: gel is bad.
| |
- | </font></p></div>
| |
- |
| |
- | <h4>August 21st</h4>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. SDS page gel electrophoresis.<br/>2. Miniprep: pSB1C3.<br/>3. Digestion: three tubes are digested with XbaI and Pst-HF, two tubes are digested with EcoRI and Pst-HF.<br/>4. SDS page gel electrophoresis.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: use PCR to clone mamL standard, mamB standard, mamQ standard, mamK standard.<br/>2. Electrophoresis: mamL, K, B gel is good; mamQ, gel is bad.<br/>
| |
- | <img src="https://static.igem.org/mediawiki/2013/b/b1/Ouc-week12.jpg" height="400" width="300" /><br/>Electrophoresis of production of mamK,B cloning<br/>
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterial activation and culturing, including control group and all experimental groups.<br/>2. Preparing for measuring the fluorescence.
| |
- | </font></p></div>
| |
- |
| |
- | <h4>August 22th</h4>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: Get mamQ sequence from PCR product and add prefix and suffix onto the squence.<br/>2. Agarose electrophoresis: detect the plasmid PCR product.<br/>3. Product purification: purify the PCR product.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterialculturing.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR amplification: mamB, four tubes; mamK, four tubes.<br/>2. SDS page gel electrophoresis.<br/>3. Gel extraction of DNA.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR Purification: PCR Purification of mamL, K, B.<br/>2. Enzyme digestion: enzyme digestion of mamL, K, B for 25ul system separately, use Xba1, Pst1.
| |
- | </font></p></div>
| |
- |
| |
- | <h4>August 23rd</h4>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Measuring the fluorescence.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Electrophoresis: production of mamL, K, B enzyme digestion, gel is good.<br/>2. Ligastion: mamL, K, B order pSB1C3 vector with chloramphenicol resistance.<br/>3. Transform: transform the ligastion product into Top 10.<br/>4. Bacterial coating.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Ligation: ligate RBS+ mamB with pSB1C3, ligate RBS+mamL and BOO15 with pSB1C3, ligate RBS+ mamK with pSB1C3.<br/>2. PCR amplification: mamB, four tubes.<br/>3. Transformation: L-P, B-P, K-P.<br/>4. SDS page gel electrophoresis.<br/>5. Ligation: ligate the purified mamC and mamE with T vector.<br/>6. Transformation: C-T, E-T.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: Get mamI sequence from PCR product and add prefix and suffix onto the sequence.<br/>2. PCR: Get mamL sequence from PCR product and add prefix and suffix onto the sequence.<br/>3. PCR: Get mamB sequence from PCR product and add prefix and suffix onto the sequence.<br/>4. Agarose electrophoresis: detect the plasmid PCR product.<br/>5. Product purification: purify the PCR product.
| |
- | </font></p></div>
| |
- |
| |
- | <h4>August 24th</h4>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: amplification mamB+mamL, mamQ+mamI sequence from DNA ligastion product.<br/>2. Agarose electrophoresis: detect the plasmid PCR product.<br/>3. PCR: Get mamI sequence from AMB-1 bacterial strain.<br/>4. PCR: Get mamI sequence from PCR product and add prefix and suffix onto the sequence.<br/>5. Product purification: purify the PCR product.<br/>
| |
- | 6. Agarose electrophoresis: detect the plasmid PCR product.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick the bacteria and culture the E.coli cells for four hours.<br/>2. PCR: C-T, E-T, L-P, B-P, K-P.<br/>3. SDS page gel electrophoresis: PCR production.<br/>
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria: pick bacteria we want (mamL, K, B-PSB1C3).<br/>2. PCR: use PCR to detect mamL, K, B-PSB1C3 vector1~10, 25ul.<br/>3. Electrophoresis: gel is bad.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Result analysis.
| |
- | </font></p></div>
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </div>
| |
- | <br /><br /></br>
| |
- | </section>
| |
- |
| |
- | <section id="Week10">
| |
- | <div class="page-header">
| |
- | <h1>Week10</h1>
| |
- | </div>
| |
- | <div class="row">
| |
- |
| |
- | <div class="span9">
| |
- | <div class="row">
| |
- | <h4>August 25th</h4>
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterial culturing, including control group and all experimental group.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/> 1. Ligastion: mamC, mamE order pMD19-T vector. <br/>2. Transform: transform the ligastion product into Top 10.<br/>3. Bacterial coating.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: L-P.<br/>2. SDS page gel electrophoresis: mamC; mamE.<br/>
| |
- | <img src="https://static.igem.org/mediawiki/2013/3/3f/Ouc-week13.png" height="400" width="300" /><br/>lane5: mamC, lane6: mamE<br/>
| |
- | </font></p></div>
| |
- |
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: Get mamJ sequence from AMB-1 bacterial strain.<br/>2. Digestion: use enzyme XbaI I, Spel I to cut mamI, mamL, mamB, mamQ.<br/>3. Plasmid Extraction: extract the pSB1C3 plasmid which containing B0015 from E.coli.<br/>4. Agarose electrophoresis: detect the plasmid extraction product and PCR product.<br/>5. Ligastion: mamB and mamL; mamQ and mamI.<br/>6. Agarose electrophoresis: detect the DNA digestion product.<br/>7. Bacterial inoculation: inoculate the E.coli which contain pSB1C3 vector plasmid, C+.<br/>8. PCR: amplification mamB+mamL, mamQ+mamI sequence from DNA ligastion product.<br/>9. Agarose electrophoresis: detect the plasmid PCR product.
| |
- | </font></p></div>
| |
- |
| |
- | <h4>August 26th</h4>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Plasmid minprep.<br/>2. Enzyme digestion and electrophoresis examining.<br/>3. Recombination.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria: pick bacteria we want (mamC, E-pMD19T- vector).<br/>2. PCR: use PCR to detect mamC, E-pMD19T- vector1~10, 25ul.<br/>3. Electrophoresis: gel is bad.<br/>4. Pick bacteria: pick bacteria we want(mamC,E-PMD19T- vector ).
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick the bacteria and culture the E.coli cells for four hours.<br/>2. PCR: Q-P, K-P.
| |
- | </font></p></div>
| |
- |
| |
- | <h4>August 27th</h4>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick the bacteria and culture the E.coli cells for four hours: K-P.<br/>2. PCR: Q-P, K-P.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Transformation.
| |
- | </font></p></div>
| |
- |
| |
- |
| |
- | <h4>August 28th</h4>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR amplification: mamB, four tubes.<br/>2. SDS page gel electrophoresis.
| |
- | </font></p></div>
| |
- |
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: use PCR to clone mamC standard.<br/>2. PCR Purification: PCR Purification of mamL, K, B.<br/>3. Electrophoresis: gel is good.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR amplification: mamB, four tubes.<br/>2. SDS page gel electrophoresis.
| |
- | </font></p></div>
| |
- |
| |
- | <h4>August 29th</h4>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria.<br/>2. PCR.<br/>3. Electrophoresis examining.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Digestion: digest RBS+mamK with XbaI and Pst-HF, digest RBS+mamB with XbaI and Pst-HF.<br/>2. PCR amplification: mamE, three tubes; mamQ, three tubes, mamL, two tubes.<br/>3. SDS page gel electrophoresis.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR Purification: PCR Purification of mamE, L, Q.<br/>2. Electrophoresis: gel is good.
| |
- | </font></p></div>
| |
- |
| |
- |
| |
- | <h4>August 30th</h4>
| |
- |
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria, PCR and electrophoresis examining.<br/>2. We find none of the fragment conforms to the aimed length, we explain it by the wrong proportion of part and plasmid backbone, so we prepared another experiment.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: use PCR to clone mamI standard.<br/>2. Ligastion: mamL order pSB1C3 vector with chloramphenicol resistant.<br/>3. Bacterination: bacterinate JB11 (promoter-RBS-PSB1C3), into 3 tubes of 10ml each.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. SDS page gel electrophoresis.<br/>2. Digestion: digest RBS+mamL with XbaI and Pst-HF, digest RBS+mamQ with XbaI and Pst-HF.<br/>3. PCR amplification: mamQ, two tubes; mamK, two tubes.
| |
- | </font></p></div>
| |
- |
| |
- | <h4>August 31st</h4>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. SDS page gel electrophoresis.<br/>
| |
- | 2. PCR amplification: mamB, two tubes; mamK, two tubes.<br/>3. PCR production purification: RBS+mamB, two tubes.<br/>4. Digestion: digest RBS+mamB with XbaI and Pst-HF.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Plasmid miniprep: plasmid extraction of E.coli with JB11 (promoter-RBS-PSB1C3) plasmid for 6 tubes.<br/>2. Enzyme digestion: enzyme digestion of JB11 (promoter-RBS-PSB1C3) for 25ul system, use Spe1, Pst1; enzyme digestion of mamQ for 25ul system, use Xba1, Pst1.enzyme digestion of mamB for 25ul system, use Xba1, Pst1.<br/>3. PCR Purification: PCR Purification of mamC.<br/>4. Electrophoresis: gel is good.<br/>5. Ligastion: mamC, E order pMD19T-vector.<br/> 6. Transform: transform the ligastion product into Top 10.<br/>7. Bacterial coating.<br/>8. Ligastion:Promoter - RBS order mamQ, promoter-RBS order mamB.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterial culturing, including control group and all experimental groups.
| |
- | </font></p></div>
| |
- |
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </div>
| |
- | <br /><br /></br>
| |
- | </section>
| |
- |
| |
- | <section id="Week11">
| |
- | <div class="page-header">
| |
- | <h1>Week11</h1>
| |
- | </div>
| |
- | <div class="row">
| |
- |
| |
- | <div class="span9">
| |
- | <div class="row">
| |
- | <h4>Septembert 1st</h4>
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Plasmid minprep.<br/>2. Enzyme digestion and electrophoresis examining.<br/>3. Recombination.
| |
- | </font></p></div>
| |
- |
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria: pick bacteria we want (mamL, pSB1C3 vector ).<br/>2.Transform: transform promoter-RBS-mamB, promoter-RBS-mamQ, mamK-PSB1C3 into Top10.<br/>3. PCR: use PCR to detect mamK-PSB1C3, 25ul system.
| |
- | </font></p></div>
| |
- |
| |
- | <h4>September 2nd</h4>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Transformation.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Electrophoresis: mamL1~20, 3, 12, 14. gel is good.<br/>
| |
- | <img src="https://static.igem.org/mediawiki/2013/9/94/Ouc-week14.jpg" height="400" width="300" /><br/>Electrophoresis of mamL<br/>2. Pick bacteria: pick bacteria we want (promoter-RBS-mamB, promoter-RBS-mamQ).<br/>3. PCR: use PCR to detect promoter-RBS-mamB, promoter-RBS-mamQ.
| |
- | </font></p></div>
| |
- |
| |
- | <h4>September 3rd</h4>
| |
- |
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Grow bacteria in the culture plates.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterial coating: mamK-PSB1C3.
| |
- | </font></p></div>
| |
- |
| |
- | <h4>September 4th</h4>
| |
- |
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria: pick bacteria we want (mamK-PSB1C3).<br/>2. PCR: use PCR to detect (mamK-PSB1C3).<br/>3. Electrophoresis: mamK-PSB1C3 number5, gel is good, others are bad.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick up the single colony, PCR, run gel and sequencing.<br/>2. Miniprep of B0015 and four experiment groups.<br/>3. Cultured cells of PSB1C3 for making the backbone.
| |
- | </font></p></div>
| |
- |
| |
- | <h4>September 5th</h4>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Miniprep the PSB1C3.<br/>2. Enzyme digestion: B0015 with EcoR I and SpeI; the experiment group 1 use XbaI and PstI; the other experiments group use EcoR I and XbaI; the PSB1C3 with EcoRI and PstI.<br/>3. Gel extraction PSB1C3 and the experiment group 1.<br/>4. Recombination.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria: pick bacteria we want (mamK-PSB1C3).<br/>2. PCR: use PCR to detect (mamK-PSB1C3).<br/>3. Electrophoresis: gel is bad.
| |
- | </font></p></div>
| |
- |
| |
- | <h4>September 6th</h4>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Transformation.
| |
- | </font></p></div>
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </div>
| |
- | <br /><br /></br>
| |
- | </section>
| |
- |
| |
- | <section id="Week12">
| |
- | <div class="page-header">
| |
- | <h1>Week12</h1>
| |
- | </div>
| |
- | <div class="row">
| |
- |
| |
- | <div class="span9">
| |
- | <div class="row">
| |
- | <h4>September 9th</h4>
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick up single colony, PCR, and sequencing.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: use PCR to clone mamC.<br/>2. Electrophoresis: gel is bad.<br/>3. Enzyme digestion: enzyme digestion of mamI, mamQ; 25ul system, use Xba1, Pst1.<br/>4. Electrophoresis: gel is good.
| |
- | </font></p></div>
| |
- |
| |
- | <h4>September 10th</h4>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Culture the experiment group 1.
| |
- | </font></p></div>
| |
- |
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria: pick bacteria we want (mamQ1~40, mamI1~10).<br/>2. PCR: use PCR to detect mamQ, mamI.<br/>3. Electrophoresis: gel is good.<br/>
| |
- | <img src="https://static.igem.org/mediawiki/2013/c/c2/Ouc-week15.jpg" height="400" width="300" /><br/>Electrophoresis of mamI<br/>
| |
- | </font></p></div>
| |
- |
| |
- | <h4>September 11st</h4>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Miniprep and enzyme digestion with EcoR I and XbaI.<br/>2. Gel extraction.
| |
- | </font></p></div>
| |
- |
| |
- | <h4>September 12th</h4>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterination: Bacterinate E.coli with pSB2K3-RFP into tubes of 10ml.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xiaodong Zhong
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. TEM (Electron microscopy) of Magnetospirillum Magneticum AMB-1.<br/>
| |
- | <img src="https://static.igem.org/mediawiki/2013/a/af/Ouc-week16.jpg" height="400" width="300" /><br/>
| |
- | </font></p></div>
| |
- |
| |
- | <h4>September 13th</h4>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Plasmid miniprep: plasmid extraction of pSB2K3 for 5 tubes.<br/>2. Enzyme digestion: enzyme digestion of pSB2K3 for 50ul system, use EcoR1, Pst1.<br/>3. Gel recovery: gel recovery of the product of enzyme digestion.<br/>
| |
- | 4. Electrophoresis: gel is good.
| |
- | </font></p></div>
| |
- |
| |
- | <h4>September 14th</h4>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Inoculate the cell to culture plates, containing the control group, experiment 3 and experiment 4.
| |
- | </font></p></div>
| |
- |
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </div>
| |
- | <br /><br /></br>
| |
- | </section>
| |
- |
| |
- | <section id="Week13">
| |
- | <div class="page-header">
| |
- | <h1>Week13</h1>
| |
- | </div>
| |
- | <div class="row">
| |
- |
| |
- | <div class="span9">
| |
- | <div class="row">
| |
- | <h4>September 15th</h4>
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Fluorescence detection.
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Microscopy of Magnetospirillum magneticum AMB-1.<br/>
| |
- | <img src="https://static.igem.org/mediawiki/2013/e/e3/Ouc-week17.jpg" height="400" width="300" /><br/>
| |
- | </font></p></div>
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xiaodong Zhong
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Magnetic analysis: detect the magnetism of Magnetospirillum magneticum AMB-1.
| |
- | <div class="span4"><img src="https://static.igem.org/mediawiki/2013/e/e7/Ouc-week18.jpg" height="400" width="300" /></div>
| |
- | <div class="span4"><img src="https://static.igem.org/mediawiki/2013/4/44/Ouc-week19.jpg" height="400" width="300" /></div><br/><br/>
| |
- | <div class="span4"><img src="https://static.igem.org/mediawiki/2013/f/f6/Ouc-week20.jpg" height="400" width="300" /></div>
| |
- | <div class="span4"><img src="https://static.igem.org/mediawiki/2013/6/6f/Ouc-week21.jpg" height="400" width="300" /></div>
| |
- | </font></p></div>
| |
- |
| |
- | <h4>September 20th</h4>
| |
- |
| |
- |
| |
- | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| |
- | </font></p>
| |
- | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Plasmid miniprep: plasmid Extraction of double expression plasmid for 9 tubes.<br/>2. Electrophoresis: gel is good.<br/>
| |
- | <img src="https://static.igem.org/mediawiki/2013/1/13/Ouc-week22.jpg" height="400" width="300" /><br/>Electrophoresis of double expression plasmid<br/>
| |
- | </font></p></div>
| |
- |
| |
- |
| |
- |
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </div>
| |
- | <br /><br /></br>
| |
- | </section>
| |
- |
| |
- | </div>
| |
- | </div>
| |
- | </div>
| |
| | | |
| | | |