Team:OUC-China/Lab note
From 2013.igem.org
Lab-note
Week1
June 26th
Person: Wenjun Wang
Experiment:
1. Get the AMB-1 bacteria strain from Nanjing University.
2. Keep the strain in 30℃.
Week2
June 30th
Person: Wengjun Wang
Experiment:
1. PCR: Get 16srDNA sequence from AMB-1 bacteria genome to detect the strain.
2. Agarose electrophoresis: detect the PCR product.
3. Transform: transform the ligastion product into Top 10 to prepare for sequence.
Week3
July 13th ~ 15th
Person: Wenjun Wang
Experiment:
1. Get the AMB-1 bacteria strain from Nanjing University.
2. Keep the strain in 30℃.
Week4
June 30th
Person: Kaili Qin
Experiment:
1. Equip the medium: Equip the medium of Magnetospirillum Magneticum AMB-1.
July 20th
Person: Wenjun Wang
Experiment:
1. PCR: Get mamJ sequence from PCR product to finish the Over-Lap PCR.
2. Agarose electrophoresis: detect the plasmid PCR product.
3. Transform: transform the ligastion product into Top 10 to prepare for sequence.
4. PCR: Get mamK sequence from PCR product to finish the Over-Lap PCR.
5. Agarose electrophoresis: detect the PCR product.
6. Product purification: purify the PCR product.
7. PCR: Get mamAB sequence from AMB-1 bacteria genome.Person: Qianyun Lu
1. Culture the E.coli cells: Psb1c3.