Team:Peking/HumanPractice/ModeliGEM

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<a href="https://2013.igem.org/Team:Peking">Home</a>
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<li><a href="https://2013.igem.org/Team:Peking/Team/Members">Members</a></li>
<li><a href="https://2013.igem.org/Team:Peking/Team/Members">Members</a></li>
<li><a href="https://2013.igem.org/Team:Peking/Team/Notebook">Notebook</a></li>
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<a href="https://2013.igem.org/Team:Peking/Project">Project</a>
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                                 <li><a href="https://2013.igem.org/Team:Peking/Project/SensorMining">Biosensor Mining</a></li>
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<li><a href="https://2013.igem.org/Team:Peking/Project/BioSensors">Biosensors</a></li>
<li><a href="https://2013.igem.org/Team:Peking/Project/BioSensors">Biosensors</a></li>
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<li><a href="https://2013.igem.org/Team:Peking/Project/Plugins">Adaptors</a></li>
<li><a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">Band-pass Filter</a></li>
<li><a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">Band-pass Filter</a></li>
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                                <li><a href="https://2013.igem.org/Team:Peking/Project/Devices">Devices</a></li>
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<a href="https://2013.igem.org/Team:Peking/Model">Model</a>
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<a href="">Model</a>
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                         <li id="PKU_navbar_HumanPractice" class="Navbar_Item" style="width:90px">
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<li><a href="https://2013.igem.org/Team:Peking/DataPage/JudgingCriteria">Judging Criteria</a></li>
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<li id="PKU_navbar_HumanPractice" class="Navbar_Item" style="width:120px">
<a href="https://2013.igem.org/Team:Peking/HumanPractice">Human Practice</a>
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<li><a href="https://2013.igem.org/Team:Peking/HumanPractice/FactoryVisit">Visit and Interview</a></li>
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                                 <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/ModeliGEM">Practical Analysis</a></li>
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<li><a href="https://2013.igem.org/Team:Peking/HumanPractice/iGEMWorkshop">Team Communication</a></li>
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         <a href="https://2013.igem.org/Main_Page"><img id="iGEM_logo" src="https://static.igem.org/mediawiki/igem.org/4/48/Peking_igemlogo.jpg"/></a>
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                 <h1 id="HumanPracticeListTitle"><a href="https://2013.igem.org/Team:Peking/HumanPractice">Human Practice</a></h1>
                 <h1 id="HumanPracticeListTitle"><a href="https://2013.igem.org/Team:Peking/HumanPractice">Human Practice</a></h1>
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<B><I>In September 7th, the first Model iGEM was hosted by Peking iGEM. Focusing on inter-team communication, preparing for iGEM jamboree but not for prize, Model iGEM is a competition without competitiveness. </I></B>
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<I><B>
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In order to test whether our toolkit works practically, we took water samples from our beloved Weiming Lake. Is she pure as she appears? Or is she polluted by aromatic compounds?
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</B></I>
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</br></br>
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Weiming Lake is a famous scenic spot in Peking University. In Chinese, "Weiming" means "too beautiful to be named". Many people in history failed to name it so a famous scholar gave the lake this name.
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We believe, that interactions bring great insights. And we all think it’s a really unique, fantastic experience. We hope other iGEM teams are inspired to do the same, and Model iGEM will be continued to host in the years after.
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</br>Located in the center of the campus, the lake was once a royal garden in Qing Dynasty. Today it is still regarded as a sacred lake by many visitors. On the hill of the southern part to Weiming Lake, there is a pagoda named Boya. It is an imitation of ancient tower. The combination of the lake and the tower forms a distinctive landscape. Students in Peking University can be seen reading and studying beside the lake.</br></br>
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<br/><br/>
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Thus,testing water sample from this lake is meaningful for us.
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For several team members, it will be their first time to attend iGEM Asian Jamboree in this October. Model iGEM could be seen as a “drill” in advance, from presentation to poster. We want the competition to work like the Asian Jamboree as much as possible, in the meanwhile, promote the communication between teams.<br/>
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</br></br>
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However, it’s inappropriate and unnecessary to award any prize, for few teams have adequate time to get ready in early September.
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<br/><br/>
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<B>Plans</B><br/>
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Opening Ceremony<br/>
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8:40 a.m.       Opening Remarks by Dr. Chunbo Lou<br/>
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Presentation Competition<br/>
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9:15- 9:45      Pre of Peking<br/>
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9:45- 10:15    Pre of BIT<br/>
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10:15-10:45    Pre of Fudan<br/>
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10:45-11:00    <I>Tea break</I><br/>
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11:00-11:30    Pre of NJU-China<br/>
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11:30-12:00    Pre of Tianjin<br/>
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12:00-12:30    Pre of OUC-China<br/><br/>
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Each team has 20 minutes for presentation, 5 minutes for Q&A and another 5 minutes for team-switching during Presentation Competition.
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Poster Competition<br/>
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12:30-14:00    Lunch at posters<br/><br/>
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Closing Ceremony<br/>
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14:30-15:30  Closing remarks by Haoqian Zhang<br/>
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            Team communication and group photo-taking<br/><br/>
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<B>Snap shot</B><br/>
 
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Concentrating on ideas from different teams, we really enjoyed our time. Strengths and weaknesses, we sometimes saw in ourselves and from others. Wonderful questions from audiences and judges pointed out how to improve and present our projects in the future, so does advice.
 
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<img id="FigurePic2" src="https://static.igem.org/mediawiki/igem.org/1/1b/Peking2013_Testingwater.jpg"/>
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Having experienced a model competition, we owned a chance to view our project as an outsider. And we believe that, we will be much more at ease in the presentation of Asian jamboree.  
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</br>Water from Weiming Lake in Peking University was sampled at 15:30 and 19:30, sterilized by 0.22 um filter. LB solution was prepared by using each of the water samples. Antibiotics were added into two tubes containing the two water samples to prepare 50 ml engineered bacteria medium. After 12 hours cultivation according to the inducing process, OD<sub>600</sub> and fluorescence<sub>515</sub> of the samples were tested. Then, aromatic compounds were calculated by dose-response curves.
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<B><I>Methods</B></I>
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As for lunchtime, we enjoyed the tasty pizza and our talk. We shared funny stories in this summer and exchanged ideas about different projects as well as teams. Walking around, we viewed posters from each team, and discussed curves, data, even details about materials and test conditions.
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Eventually, closing remarks approached. Tired but joyful, we will never forget this rewarding model jamboree.
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<B>Strains and growth media. </B></br>
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<I>E.coli</I> Top10 was used for all the experiments and cultured in Luria–Bertani (LB) medium. Kanamycin (10 μg/mL), ampicillin (50 μg/mL) and chloramphenicol (170μg/mL) were added as appropriate.
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<B>Protocol </B></br>
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<I>E.coli</I> was grown in LB medium overnight at 37 °C and then diluted 100-fold in fresh LB medium in 96-well plates (Corning Incorporated, 3599). Then each culture (200 μL) was induced for 12 hours at 30°C with inducers of different concentrations. Then the fluorescence intensity of cultures was measured by a microplate reader (Thermo) or LSRFortessa flow cytometer (BD Biosciences).
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<B><I>Result</B></I>
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Team Communication
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The concentration of aromatic compounds was parallelly measured three times with a microplate reader and the induction ratios are showed below. The result shows that the concentration of benzoates, salicylic acids, and biphenyl derivatives to which biosensor XylS, NahR and HbpR response respectively is lower than the detecting limit of our biosensors. The Weiming lake is free from these kinds of aromatic pollution!
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<img id="FigurePic3" src="https://static.igem.org/mediawiki/igem.org/c/c6/Peking2013_Testwaterresult.jpg"/>
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Latest revision as of 18:20, 28 October 2013

Practical Analysis

In order to test whether our toolkit works practically, we took water samples from our beloved Weiming Lake. Is she pure as she appears? Or is she polluted by aromatic compounds?

Weiming Lake is a famous scenic spot in Peking University. In Chinese, "Weiming" means "too beautiful to be named". Many people in history failed to name it so a famous scholar gave the lake this name.




Located in the center of the campus, the lake was once a royal garden in Qing Dynasty. Today it is still regarded as a sacred lake by many visitors. On the hill of the southern part to Weiming Lake, there is a pagoda named Boya. It is an imitation of ancient tower. The combination of the lake and the tower forms a distinctive landscape. Students in Peking University can be seen reading and studying beside the lake.

Thus,testing water sample from this lake is meaningful for us.


Water from Weiming Lake in Peking University was sampled at 15:30 and 19:30, sterilized by 0.22 um filter. LB solution was prepared by using each of the water samples. Antibiotics were added into two tubes containing the two water samples to prepare 50 ml engineered bacteria medium. After 12 hours cultivation according to the inducing process, OD600 and fluorescence515 of the samples were tested. Then, aromatic compounds were calculated by dose-response curves.


Methods


Strains and growth media.
E.coli Top10 was used for all the experiments and cultured in Luria–Bertani (LB) medium. Kanamycin (10 μg/mL), ampicillin (50 μg/mL) and chloramphenicol (170μg/mL) were added as appropriate.

Protocol
E.coli was grown in LB medium overnight at 37 °C and then diluted 100-fold in fresh LB medium in 96-well plates (Corning Incorporated, 3599). Then each culture (200 μL) was induced for 12 hours at 30°C with inducers of different concentrations. Then the fluorescence intensity of cultures was measured by a microplate reader (Thermo) or LSRFortessa flow cytometer (BD Biosciences).

Result


The concentration of aromatic compounds was parallelly measured three times with a microplate reader and the induction ratios are showed below. The result shows that the concentration of benzoates, salicylic acids, and biphenyl derivatives to which biosensor XylS, NahR and HbpR response respectively is lower than the detecting limit of our biosensors. The Weiming lake is free from these kinds of aromatic pollution!