"
Team:Peking/Project
From 2013.igem.org
(Difference between revisions)
XingjiePan (Talk | contribs) |
|||
| (16 intermediate revisions not shown) | |||
| Line 139: | Line 139: | ||
.Navbar_Item > ul > li {float:left; list-style:none; text-align:center; background-color:transparent; position:relative; top:10px; padding:0 10px;} | .Navbar_Item > ul > li {float:left; list-style:none; text-align:center; background-color:transparent; position:relative; top:10px; padding:0 10px;} | ||
.Navbar_Item:hover{ border-bottom:1px solid #D00000; color:#D00000; background-color:#fafaf8;} | .Navbar_Item:hover{ border-bottom:1px solid #D00000; color:#D00000; background-color:#fafaf8;} | ||
| - | .Navbar_Item:hover > ul{ display:block;} | + | .Navbar_Item:hover > ul{zoom:1; display:block;} |
.Navbar_Item:hover >a {color:#D00000; background-color:#fafaf8;} | .Navbar_Item:hover >a {color:#D00000; background-color:#fafaf8;} | ||
.Navbar_Item > ul > li:hover {border-bottom:1px solid #D00000; color:#D00000; background-color:#fafaf8;} | .Navbar_Item > ul > li:hover {border-bottom:1px solid #D00000; color:#D00000; background-color:#fafaf8;} | ||
.Navbar_Item > ul > li:hover >a {color:#D00000} | .Navbar_Item > ul > li:hover >a {color:#D00000} | ||
| + | .BackgroundofSublist{position:absolute; left:-1000px; width:2000px; height:80px; background-color:#ffffff; opacity:0;} | ||
| Line 151: | Line 152: | ||
#DataPage_Sublist{position:relative; top:0px; left:-60px;} | #DataPage_Sublist{position:relative; top:0px; left:-60px;} | ||
#Safety_Sublist{position:relative; top:0px; left:-180px;} | #Safety_Sublist{position:relative; top:0px; left:-180px;} | ||
| - | #HumanPractice_Sublist{position:relative; top:0px; left:- | + | #HumanPractice_Sublist{position:relative; top:0px; left:-470px;} |
#iGEM_logo{position:absolute; top:30px; left:1090px; height:80px;} | #iGEM_logo{position:absolute; top:30px; left:1090px; height:80px;} | ||
| Line 157: | Line 158: | ||
/*Major body*/ | /*Major body*/ | ||
| - | #MajorBody{position:absolute; top:24px; left:0px; width:1200px; height: | + | #MajorBody{position:absolute; top:24px; left:0px; width:1200px; height:1200px; background-color:#ffffff;} |
| - | #LeftNavigation{position:fixed; top:130px; float:left; width:200px; height:100%; background-color:#313131} | + | #LeftNavigation{position:fixed; top:130px; float:left; width:200px; height:100%; background-color:#313131; z-index:1050;} |
#ProjectList{position:absolute; top:40px; left:0px; color:#ffffff; font-family: calibri, arial, helvetica, sans-serif;} | #ProjectList{position:absolute; top:40px; left:0px; color:#ffffff; font-family: calibri, arial, helvetica, sans-serif;} | ||
#ProjectList > li {display:block; list-style-type:none; width:180px; height:30px; font-size:20px; text-align:left; background-color:transparent;} | #ProjectList > li {display:block; list-style-type:none; width:180px; height:30px; font-size:20px; text-align:left; background-color:transparent;} | ||
| - | + | .SensorsListItem>a {color:#ffffff; text-decoration:none;} | |
| + | .SensorsListItem:hover >a{color:#999999;} | ||
| Line 187: | Line 189: | ||
<ul id="navigationbar"> | <ul id="navigationbar"> | ||
<li id="PKU_navbar_Home" class="Navbar_Item"> | <li id="PKU_navbar_Home" class="Navbar_Item"> | ||
| + | |||
<a href="https://2013.igem.org/Team:Peking">Home</a> | <a href="https://2013.igem.org/Team:Peking">Home</a> | ||
<ul id="Home_Sublist" > | <ul id="Home_Sublist" > | ||
| Line 192: | Line 195: | ||
</li> | </li> | ||
<li id="PKU_navbar_Team" class="Navbar_Item"> | <li id="PKU_navbar_Team" class="Navbar_Item"> | ||
| - | <a >Team</a> | + | <a href="">Team</a> |
<ul id="Team_Sublist"> | <ul id="Team_Sublist"> | ||
| + | <div class="BackgroundofSublist"></div> | ||
<li><a href="https://2013.igem.org/Team:Peking/Team/Members">Members</a></li> | <li><a href="https://2013.igem.org/Team:Peking/Team/Members">Members</a></li> | ||
<li><a href="https://2013.igem.org/Team:Peking/Team/Notebook">Notebook</a></li> | <li><a href="https://2013.igem.org/Team:Peking/Team/Notebook">Notebook</a></li> | ||
| Line 202: | Line 206: | ||
<a href="https://2013.igem.org/Team:Peking/Project">Project</a> | <a href="https://2013.igem.org/Team:Peking/Project">Project</a> | ||
<ul id="Project_Sublist"> | <ul id="Project_Sublist"> | ||
| + | <div class="BackgroundofSublist"></div> | ||
<li><a href="https://2013.igem.org/Team:Peking/Project/SensorMining">Biosensor Mining</a></li> | <li><a href="https://2013.igem.org/Team:Peking/Project/SensorMining">Biosensor Mining</a></li> | ||
<li><a href="https://2013.igem.org/Team:Peking/Project/BioSensors">Biosensors</a></li> | <li><a href="https://2013.igem.org/Team:Peking/Project/BioSensors">Biosensors</a></li> | ||
<li><a href="https://2013.igem.org/Team:Peking/Project/Plugins">Adaptors</a></li> | <li><a href="https://2013.igem.org/Team:Peking/Project/Plugins">Adaptors</a></li> | ||
<li><a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">Band-pass Filter</a></li> | <li><a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">Band-pass Filter</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:Peking/Project/Devices">Devices</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
<li id="PKU_navbar_Model" class="Navbar_Item"> | <li id="PKU_navbar_Model" class="Navbar_Item"> | ||
| - | <a href=" | + | <a href="">Model</a> |
<ul id="Model_Sublist"> | <ul id="Model_Sublist"> | ||
| + | <div class="BackgroundofSublist"></div> | ||
| + | <li><a href="https://2013.igem.org/Team:Peking/Model">Band-pass Filter</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:Peking/ModelforFinetuning">Biosensor Fine-tuning</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
<li id="PKU_navbar_HumanPractice" class="Navbar_Item" style="width:90px"> | <li id="PKU_navbar_HumanPractice" class="Navbar_Item" style="width:90px"> | ||
| - | <a >Data page</a> | + | <a href="">Data page</a> |
| - | <ul id="DataPage_Sublist"> | + | <ul id="DataPage_Sublist"> |
| + | <div class="BackgroundofSublist"></div> | ||
<li><a href="https://2013.igem.org/Team:Peking/DataPage/Parts">Parts</a></li> | <li><a href="https://2013.igem.org/Team:Peking/DataPage/Parts">Parts</a></li> | ||
<li><a href="https://2013.igem.org/Team:Peking/DataPage/JudgingCriteria">Judging Criteria</a></li> | <li><a href="https://2013.igem.org/Team:Peking/DataPage/JudgingCriteria">Judging Criteria</a></li> | ||
| Line 228: | Line 238: | ||
<li id="PKU_navbar_HumanPractice" class="Navbar_Item" style="width:120px"> | <li id="PKU_navbar_HumanPractice" class="Navbar_Item" style="width:120px"> | ||
<a href="https://2013.igem.org/Team:Peking/HumanPractice">Human Practice</a> | <a href="https://2013.igem.org/Team:Peking/HumanPractice">Human Practice</a> | ||
| - | <ul id="HumanPractice_Sublist"> | + | <ul id="HumanPractice_Sublist"> |
| - | <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/Questionnaire">Questionnaire</a></li> | + | <div class="BackgroundofSublist"></div> |
| - | <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/FactoryVisit"> | + | <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/Questionnaire">Questionnaire Survey</a></li> |
| - | + | <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/FactoryVisit">Visit and Interview</a></li> | |
| - | + | <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/ModeliGEM">Practical Analysis</a></li> | |
| + | <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/iGEMWorkshop">Team Communication</a></li> | ||
| + | |||
</ul> | </ul> | ||
</li> | </li> | ||
</ul> | </ul> | ||
| - | <a href="https://igem.org/ | + | <a href="https://2013.igem.org/Main_Page"><img id="iGEM_logo" src="https://static.igem.org/mediawiki/igem.org/4/48/Peking_igemlogo.jpg"/></a> |
</div> | </div> | ||
<!--end navigationbar--> | <!--end navigationbar--> | ||
| Line 245: | Line 257: | ||
<div id="LeftNavigation"> | <div id="LeftNavigation"> | ||
<ul id="ProjectList"> | <ul id="ProjectList"> | ||
| - | <li><a href="https://2013.igem.org/Team:Peking/Project/SensorMining"> | + | <li class="SensorsListItem"><a href="https://2013.igem.org/Team:Peking/Project/SensorMining">Biosensor Mining</a><li> |
| - | <li><a href="https://2013.igem.org/Team:Peking/Project/BioSensors">Biosensors</a><li> | + | <li class="SensorsListItem"><a href="https://2013.igem.org/Team:Peking/Project/BioSensors">Biosensors</a><li> |
| - | <li><a href="https://2013.igem.org/Team:Peking/Project/Plugins">Adaptors</a><li> | + | <li class="SensorsListItem"><a href="https://2013.igem.org/Team:Peking/Project/Plugins">Adaptors</a><li> |
| - | <li><a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">Band-pass Filter</a><li> | + | <li class="SensorsListItem"><a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">Band-pass Filter</a><li> |
| + | <li class="SensorsListItem"><a href="https://2013.igem.org/Team:Peking/Project/Devices">Devices</a><li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
| Line 260: | Line 273: | ||
Aromatic pollutants are becoming a worldwide concern. Monitoring aromatics in the environment, however, remains a substantial challenge today. Noting the power of biosensors for quick and convenient testing, Peking iGEM has developed <b>a comprehensive biosensors toolkit</b> to detect aromatics in the environment. | Aromatic pollutants are becoming a worldwide concern. Monitoring aromatics in the environment, however, remains a substantial challenge today. Noting the power of biosensors for quick and convenient testing, Peking iGEM has developed <b>a comprehensive biosensors toolkit</b> to detect aromatics in the environment. | ||
</br></br> | </br></br> | ||
| - | Transcriptional regulators sensing each general class of aromatic compounds were first <a href="https://2013.igem.org/Team:Peking/Project/SensorMining"><b>bioinformatically determined</b></a> using the genomic data from prokaryotes, and then utilized to build <a href="https://2013.igem.org/Team:Peking/Project/BioSensors"><b>biosensor circuits</b></a> in living cells. Genetic tailoring such as promoter engineering was performed to tune their properties functionally. Most of these novel biosensors proved to be capable of faithfully sensing a specific group of aromatics. Furthermore, the | + | Transcriptional regulators sensing each general class of aromatic compounds were first <a href="https://2013.igem.org/Team:Peking/Project/SensorMining"><b>bioinformatically determined</b></a> using the genomic data from prokaryotes, and then utilized to build <a href="https://2013.igem.org/Team:Peking/Project/BioSensors"><b>biosensor circuits</b></a> in living cells. Genetic tailoring such as promoter engineering was performed to tune their properties functionally. Most of these novel biosensors proved to be capable of faithfully sensing a specific group of aromatics. Furthermore, the <a href="https://2013.igem.org/Team:Peking/Project/BioSensors/MulticomponentAnalysis">synergistic/antagonistic effects</a> between their inducers was carefully examined; this allowed the combination of these biosensors to profile aromatics for the ease of practical applications. |
</br></br> | </br></br> | ||
| - | In order to expand the detection profiles of some biosensors, | + | In order to expand the detection profiles of some biosensors, aromatics-metabolizing enzymes were gleaned from natural metabolic pathways, working as <a href="https://2013.igem.org/Team:Peking/Project/Plugins"><b>Adaptors</b></a> to convert undetectable chemicals into detectable aromatics when coupled with biosensor circuits. Additionally, for the ease of practical analysis, we have constructed a genetic device called the "<a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter"><b>Band-pass Filter</b></a>" to allow the detection of analyte concentration within a specific range. Biosensors equipped with the Band-pass Filter are able to robustly quantify the aromatics in environmental samples. |
</br></br> | </br></br> | ||
In conclusion, Peking iGEM has <b>significantly enriched the repertoire of biosensors for aromatic compounds</b>. These novel biosensors, together with the Adaptors and the Band-pass Filter, will serve as intriguing synthetic biological tools for diverse practical applications, including pollution monitoring, bioremediation, and metabolic process control. | In conclusion, Peking iGEM has <b>significantly enriched the repertoire of biosensors for aromatic compounds</b>. These novel biosensors, together with the Adaptors and the Band-pass Filter, will serve as intriguing synthetic biological tools for diverse practical applications, including pollution monitoring, bioremediation, and metabolic process control. | ||
Latest revision as of 18:12, 28 October 2013
Project Overview
Aromatic pollutants are becoming a worldwide concern. Monitoring aromatics in the environment, however, remains a substantial challenge today. Noting the power of biosensors for quick and convenient testing, Peking iGEM has developed a comprehensive biosensors toolkit to detect aromatics in the environment. Transcriptional regulators sensing each general class of aromatic compounds were first bioinformatically determined using the genomic data from prokaryotes, and then utilized to build biosensor circuits in living cells. Genetic tailoring such as promoter engineering was performed to tune their properties functionally. Most of these novel biosensors proved to be capable of faithfully sensing a specific group of aromatics. Furthermore, the synergistic/antagonistic effects between their inducers was carefully examined; this allowed the combination of these biosensors to profile aromatics for the ease of practical applications. In order to expand the detection profiles of some biosensors, aromatics-metabolizing enzymes were gleaned from natural metabolic pathways, working as Adaptors to convert undetectable chemicals into detectable aromatics when coupled with biosensor circuits. Additionally, for the ease of practical analysis, we have constructed a genetic device called the "Band-pass Filter" to allow the detection of analyte concentration within a specific range. Biosensors equipped with the Band-pass Filter are able to robustly quantify the aromatics in environmental samples. In conclusion, Peking iGEM has significantly enriched the repertoire of biosensors for aromatic compounds. These novel biosensors, together with the Adaptors and the Band-pass Filter, will serve as intriguing synthetic biological tools for diverse practical applications, including pollution monitoring, bioremediation, and metabolic process control.
