Team:SJTU-BioX-Shanghai

From 2013.igem.org

(Difference between revisions)
Line 25: Line 25:
|[[Image:SJTU-BioX-Shanghai_logo.png|200px|right|frame]]
|[[Image:SJTU-BioX-Shanghai_logo.png|200px|right|frame]]
|-
|-
-
|Therefore, we choose several light-controlled gene expression systems and a newly devised tool named CRISPRi to find the solution towards this problem. By changing light intensity, we hope to regulate different genes to different certain expressing levels, thus maximizing producing efficiency. Light sensors are widely used in engineered biosynthetic parts for their sensibility and adjustable intensity, and CRISPRi can knock down different genes easily and specificly by changing the short sequence of its sgRNA part. With advantages of these two systems combined together, parameter determination of multiple gene-expressions will be more predictable and precise in our project, and this novel device will provide us with a promising way of genome-scale metabolism regulation.
+
|Thus we choose several light-controlled gene expression systems and a newly devised tool named CRISPRi to find the solution towards this problem. By changing light intensity, we hope to regulate different genes to different certain expressing levels, thus maximizing producing efficiency. Light sensors are widely used in engineered biosynthetic parts for their sensibility and adjustable intensity, and CRISPRi can knock down different genes easily and specificly by changing the short sequence of its sgRNA part. With advantages of these two systems combined together, parameter determination of multiple gene-expressions will be more predictable and precise in our project, and this novel device will provide us with a promising way of genome-scale metabolism regulation.
|[[Image:SJTU-BioX-Shanghai_team.png|right|frame|Your team picture]]
|[[Image:SJTU-BioX-Shanghai_team.png|right|frame|Your team picture]]
|-
|-

Revision as of 07:54, 30 August 2013


This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace.


Overview

Various methods have been developed to regulate gene expressions on different levels so far. Though widely used in recent study, these methods are difficult to be applied on multiple genes simultaneously and independently due to the complex procedures or the high cost. Therefore, we aim to build a versatile system to control and regulate different genes in an easy and accurate way.
Thus we choose several light-controlled gene expression systems and a newly devised tool named CRISPRi to find the solution towards this problem. By changing light intensity, we hope to regulate different genes to different certain expressing levels, thus maximizing producing efficiency. Light sensors are widely used in engineered biosynthetic parts for their sensibility and adjustable intensity, and CRISPRi can knock down different genes easily and specificly by changing the short sequence of its sgRNA part. With advantages of these two systems combined together, parameter determination of multiple gene-expressions will be more predictable and precise in our project, and this novel device will provide us with a promising way of genome-scale metabolism regulation.
Team SJTU-BioX-Shanghai


Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions