Team:SUSTC-Shenzhen-A/Project
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+ | '''Cell A:''' | ||
+ | Constant expressions: | ||
+ | '''LuxR'''(with C6HSL, activate pLux, express Zeo-r): promoter J23100 and rbs B0034 | ||
+ | '''araC'''(with arabinose, activate LasI, tetA): promoter J23100 and rbs B0034 | ||
+ | '''mCherry '''(red fluorescent protein labeled cell A): promoter pCon, rbs | ||
+ | Adjustable expressions: | ||
+ | '''Zeo-r,''' adjusted by C6HSL, produced by LuxI from '''cell B''', depends on cell B density and IPTG concentration | ||
+ | '''LasI:''' adjusted by arabinose, LasI produces C12HSL, control cell B | ||
+ | '''tetA:''' adjusted by arabinose | ||
+ | |||
+ | '''Arabinose, Zeocin, Nickel''' concentration are controlled by you | ||
+ | '''C12SHL '''concentration: controlled by cell A concentration and arabinose | ||
+ | '''Nickel '''either kill cell or slow cell growth: '''tetA''' increase sensitivity of '''cell B '''to''' Nickel''': | ||
+ | zeocin either kill cell or slow cell growth:Zeo-r decreases sensitivity of''' cell B''' to''' zeocin''' | ||
+ | '''-asv''': amino acid sequence that increase response time of LuxI, tetA and Zeo-r to regulation | ||
+ | pMB1 and p15A: control plasmid replication in E coli | ||
+ | Kan and Cam(r): with antibiotics Kanamycin and chloramphenicol, prevent lost of plasmids | ||
=== Experiment process === | === Experiment process === |
Revision as of 13:44, 26 September 2013
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