Team:SydneyUni Australia/Modelling Conclusion

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== '''Conclusions:''' ==
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From Graphs 1 and 5, one can see that 1mM of DCA is removed from solution within roughly 50 minutes when the DCA degrading cells are at a concentration of 2E8 cells/mL.
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== Conclusions: ==
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From Graphs 4, 8 and 9, it is evident that bacterial growth occurs. This growth is due to the production of glycolate, and by comparing graphs 6 and 9, one can see that bacterial growth correlates with glycolate accumulation.
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*In our model we find that 1 mM of DCA is removed from solution within roughly 150 minutes when the DCA degrading cells are at a concentration of 2 x 10<sup>8</sup> cells/mL ([https://2013.igem.org/Team:SydneyUni_Australia/Modelling_Output#graph1 graph 1] and [https://2013.igem.org/Team:SydneyUni_Australia/Modelling_Output#graph5 graph 5]).
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The cytotoxic metabolic intermediate chloroactealdehyde doesn't accumulate to a significant concentration in any of the pathways and is consistently at a negligibly small concentration. From Graphs 3 and 7 one can see that chloroacetaldehyde reaches a maximum concentration of roughly 0.2 mM in both pathways. Chloroacetaldehyde is seen to be metabolised very quickly; this concentration maximum is very short lived where it peaks at roughly 0.03 seconds and returns back to 0 mM by 0.5 seconds. It is expected that chloroacetaldehyde toxicity will not be a problem in our engineered cells.
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*It is also evident that bacterial growth can occur ([https://2013.igem.org/Team:SydneyUni_Australia/Modelling_Output#graph4 graph 4], [https://2013.igem.org/Team:SydneyUni_Australia/Modelling_Output#graph8 graph 8] and [https://2013.igem.org/Team:SydneyUni_Australia/Modelling_Output#graph9 graph 9]). This growth is due to the degradation of DCA to glycolate. We can also see that that bacterial growth correlates with glycolate accumulation (by comparing [https://2013.igem.org/Team:SydneyUni_Australia/Modelling_Output#graph6 graph 6] and [https://2013.igem.org/Team:SydneyUni_Australia/Modelling_Output#graph9 graph 9]).
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It is also possible to conclude that the pathways remove DCA at the same rate (through comparing graphs 1 and 5).  
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*The cytotoxic metabolic intermediate chloroactealdehyde does not accumulate to a significant concentration in either of the pathways and is consistently at a negligibly low concentration. We can see that chloroacetaldehyde reaches a maximum concentration of roughly 0.2 mM in both pathways ([https://2013.igem.org/Team:SydneyUni_Australia/Modelling_Output#graph3 graph 3] and [https://2013.igem.org/Team:SydneyUni_Australia/Modelling_Output#graph7 graph 7]). Chloroacetaldehyde is apparently metabolised very quickly; this concentration maximum is short lived, peaks at roughly 0.03 seconds and returns back to 0 mM by 0.5 seconds. It is expected that chloroacetaldehyde toxicity will not be a problem in our engineered cells.
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* We also conclude that the two pathways remove DCA at the same rate (by comparing [https://2013.igem.org/Team:SydneyUni_Australia/Modelling_Output#graph1 graph 1] and [https://2013.igem.org/Team:SydneyUni_Australia/Modelling_Output#graph5 graph 5]).
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== '''References:''' ==
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== References:==
[1] Krooshof, G. H., Ridder, I. S., Tepper, A. W., Vos, G. J., Rozeboom, H. J., Kalk, K. H., Dijkstra, B. W. & Janssen, D. B. (1998). Kinetic Analysis and X-ray Structure of Haloalkane Dehalogenase with a Modified Halide-Binding Site. <i>Biochemistry</i>, <b>37</b>(43), 15013-15023.
[1] Krooshof, G. H., Ridder, I. S., Tepper, A. W., Vos, G. J., Rozeboom, H. J., Kalk, K. H., Dijkstra, B. W. & Janssen, D. B. (1998). Kinetic Analysis and X-ray Structure of Haloalkane Dehalogenase with a Modified Halide-Binding Site. <i>Biochemistry</i>, <b>37</b>(43), 15013-15023.
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[6] Sinensky, M. I. (1974). Homeoviscous Adaption – A Homeostatic Process that Regulates the Viscosity of Membrane Lipids in <i>Escherichia coli</i>. <i>Proceedings from the National Academy of Science of the United States of America</i>, <b>71</b>(2), 522-525.
[6] Sinensky, M. I. (1974). Homeoviscous Adaption – A Homeostatic Process that Regulates the Viscosity of Membrane Lipids in <i>Escherichia coli</i>. <i>Proceedings from the National Academy of Science of the United States of America</i>, <b>71</b>(2), 522-525.
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[7] CyberCell Database
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[7] CyberCell Database. Retrieved from http://ccdb.wishartlab.com/CCDB/.
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[8]
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[8] Currie, R. C., Chiao, F. F., McKone, T. E. (1994) Intermedia Transfer Factors for Contaminants Found at Hazardous Waste Sites: 1,2 dichloroethane (DCA). Retrieved from http://www.dtsc.ca.gov/AssessingRisk/Upload/12dca.pdf
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[9]
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[9] Lord, J. M. (1972) Glycolate oxidoreductase in <i>Escherichia coli</i>. <i>Biochemica et Biophysica Acta</i> <b>267</b>:2, 227-327.
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[10] http://www.dtsc.ca.gov/AssessingRisk/Upload/12dca.pdf
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[10] Ishihama, Y., Schmidt, T., Rappsilber, J., Mann, M., Hartl, F. U., Kerner, M. J. & Frishman, D. (2008) Protein abundance profiling of the <i>Escherichia coli</i> cytosol. <i>BMC Genomics</i>, <b>9</b>:102.
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[11] Ishihama, Y., Schmidt, T., Rappsilber, J., Mann, M., Hartl F. U., Kerner, M. J. & Frishman, D. (2008) Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics, <b>9</b>:102
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[12] Lord, J. M. (1972) Glycolate oxidoreductase in Escherichia coli. Biochemica et Biophysica Acta <b>267</b>:2, 227-327.
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{{Team:SydneyUni_Australia/Footer}}
{{Team:SydneyUni_Australia/Footer}}

Latest revision as of 00:39, 29 October 2013

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Conclusions:

  • In our model we find that 1 mM of DCA is removed from solution within roughly 150 minutes when the DCA degrading cells are at a concentration of 2 x 108 cells/mL (graph 1 and graph 5).
  • It is also evident that bacterial growth can occur (graph 4, graph 8 and graph 9). This growth is due to the degradation of DCA to glycolate. We can also see that that bacterial growth correlates with glycolate accumulation (by comparing graph 6 and graph 9).
  • The cytotoxic metabolic intermediate chloroactealdehyde does not accumulate to a significant concentration in either of the pathways and is consistently at a negligibly low concentration. We can see that chloroacetaldehyde reaches a maximum concentration of roughly 0.2 mM in both pathways (graph 3 and graph 7). Chloroacetaldehyde is apparently metabolised very quickly; this concentration maximum is short lived, peaks at roughly 0.03 seconds and returns back to 0 mM by 0.5 seconds. It is expected that chloroacetaldehyde toxicity will not be a problem in our engineered cells.
  • We also conclude that the two pathways remove DCA at the same rate (by comparing graph 1 and graph 5).



References:

[1] Krooshof, G. H., Ridder, I. S., Tepper, A. W., Vos, G. J., Rozeboom, H. J., Kalk, K. H., Dijkstra, B. W. & Janssen, D. B. (1998). Kinetic Analysis and X-ray Structure of Haloalkane Dehalogenase with a Modified Halide-Binding Site. Biochemistry, 37(43), 15013-15023.

[2] Janecki, D. J., Bemis, K. G., Tegeler, T. J., Sanghani, P. C., Zhai, L., Hurley, T. D., Bosron, W. F. & Wang, M. (2007). A multiple reaction monitoring method for absolute quantification of the human liver alcohol dehydrogenase ADH1C1 isoenzyme. Analytical Biochemistry, 369(1), 18-26.

[3] Pandey, A. V. & Flück, C. E. (2013). NADPH P450 oxidoreductase: Structure, function, and pathology of diseases. Pharmacology & Therapeutics, 138(2), 229-254.

[4] van der Ploeg, J., Shmidt, M. P., Landa, A. S., & Janssen, D. B. (1994). Identification of Chloroacetaldehyde Dehydrogenase Involved in 1,2-Dichloroethane Degradation. Applied Environmental Microbiology 60(5), 1599-1605.

[5] van der Ploeg, J., van Hall, G. & Janssen, D. B. (1991) Characterization of the haloacid dehalogenase from Xanthobacter autotrophicus GJ10 and sequencing of the dhlB gene. Journal of Bacteriology, 173(24), 7925-33.

[6] Sinensky, M. I. (1974). Homeoviscous Adaption – A Homeostatic Process that Regulates the Viscosity of Membrane Lipids in Escherichia coli. Proceedings from the National Academy of Science of the United States of America, 71(2), 522-525.

[7] CyberCell Database. Retrieved from http://ccdb.wishartlab.com/CCDB/.

[8] Currie, R. C., Chiao, F. F., McKone, T. E. (1994) Intermedia Transfer Factors for Contaminants Found at Hazardous Waste Sites: 1,2 dichloroethane (DCA). Retrieved from http://www.dtsc.ca.gov/AssessingRisk/Upload/12dca.pdf

[9] Lord, J. M. (1972) Glycolate oxidoreductase in Escherichia coli. Biochemica et Biophysica Acta 267:2, 227-327.

[10] Ishihama, Y., Schmidt, T., Rappsilber, J., Mann, M., Hartl, F. U., Kerner, M. J. & Frishman, D. (2008) Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics, 9:102.


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