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Team:TU-Munich/Notebook/Materials
From 2013.igem.org
(Difference between revisions)
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|Name | |Name | ||
|Sequence | |Sequence | ||
| + | | | ||
|- | |- | ||
| + | | | ||
|EreA_for | |EreA_for | ||
|<code>5’- GCGCGTCTAGATGGCCGGCACGTGGAGAACGACCAGA -3‘</code><br> | |<code>5’- GCGCGTCTAGATGGCCGGCACGTGGAGAACGACCAGA -3‘</code><br> | ||
|- | |- | ||
| + | | | ||
|EreA-rev | |EreA-rev | ||
|<code>5‘- TTCGAAACCGGTTAGGGCAACTAGGCTGTCC -3‘</code><br> | |<code>5‘- TTCGAAACCGGTTAGGGCAACTAGGCTGTCC -3‘</code><br> | ||
|- | |- | ||
| + | | | ||
|EreA_SDMI_for | |EreA_SDMI_for | ||
|<code>5’- CGAATCAGGAAGAAAACTACAGTTAGTCGGAATCGAC -3‘</code><br> | |<code>5’- CGAATCAGGAAGAAAACTACAGTTAGTCGGAATCGAC -3‘</code><br> | ||
|- | |- | ||
| + | | | ||
|EreA_SDMI_rev | |EreA_SDMI_rev | ||
|<code>5'- GTCGATTCCGACTAACTGTAGTTTTCTTCCTGATTCG -3'</code><br> | |<code>5'- GTCGATTCCGACTAACTGTAGTTTTCTTCCTGATTCG -3'</code><br> | ||
|- | |- | ||
| + | | | ||
|EreA_SDMII_for | |EreA_SDMII_for | ||
|<code>5'- GACGCCTGTGGTACGGAAAATTCATGTCTGACATT - 3'</code><br> | |<code>5'- GACGCCTGTGGTACGGAAAATTCATGTCTGACATT - 3'</code><br> | ||
|- | |- | ||
| + | | | ||
|EreA_SDMII_rev | |EreA_SDMII_rev | ||
|<code>5'- AATGTCAGACATGAATTTTCCGTACCACAGGCGTC - 3'</code><br> | |<code>5'- AATGTCAGACATGAATTTTCCGTACCACAGGCGTC - 3'</code><br> | ||
|- | |- | ||
| + | | | ||
|EreA_SDMIII_for | |EreA_SDMIII_for | ||
|<code>5'- GTGTTCCAAGCGCGGGCAAGGACAGCC - 3'</code><br> | |<code>5'- GTGTTCCAAGCGCGGGCAAGGACAGCC - 3'</code><br> | ||
|- | |- | ||
| + | | | ||
|EreA_SDMIII_rev | |EreA_SDMIII_rev | ||
|<code>5'- GGCTGTCCTTGCCCGCGCTTGGAACAC - 3'</code><br> | |<code>5'- GGCTGTCCTTGCCCGCGCTTGGAACAC - 3'</code><br> | ||
|- | |- | ||
| + | | | ||
| EreB_for | | EreB_for | ||
|<code>5'- GCGCGTCTAGATGGCCGGCAGGTTCGAAGAATGGGTC - 3'</code><br> | |<code>5'- GCGCGTCTAGATGGCCGGCAGGTTCGAAGAATGGGTC - 3'</code><br> | ||
|- | |- | ||
| + | | | ||
| EreB-rev | | EreB-rev | ||
|<code>5'- TTCGAAACCGGTTTCATAAACGACCTCAGATACAG - 3'</code><br> | |<code>5'- TTCGAAACCGGTTTCATAAACGACCTCAGATACAG - 3'</code><br> | ||
|- | |- | ||
| + | | | ||
|EreB_SDMI_for | |EreB_SDMI_for | ||
|<code>5'- GGTGAAAATTCTCATTTCATAAAAGAGTTCTTTTTGTTACGACATACGCTTTT - 3'</code><br> | |<code>5'- GGTGAAAATTCTCATTTCATAAAAGAGTTCTTTTTGTTACGACATACGCTTTT - 3'</code><br> | ||
|- | |- | ||
| + | | | ||
|EreB_SDMI_rev | |EreB_SDMI_rev | ||
|<code>5'- AAAAGCGTATGTCGTAACAAAAAGAACTCTTTTATGAAATGAGAATTTTCACC - 3'</code><br> | |<code>5'- AAAAGCGTATGTCGTAACAAAAAGAACTCTTTTATGAAATGAGAATTTTCACC - 3'</code><br> | ||
|- | |- | ||
| + | | | ||
|EreB_SDMII_for | |EreB_SDMII_for | ||
|<code>5'- CTTATAGTGGGCATACAGCAGCCCTCTATCCGG - 3'</code><br> | |<code>5'- CTTATAGTGGGCATACAGCAGCCCTCTATCCGG - 3'</code><br> | ||
|- | |- | ||
| + | | | ||
| EreB_SDMII_rev | | EreB_SDMII_rev | ||
|<code>5'- CCGGATAGAGGGCTGCTGTATGCCCACTATAAG - 3'</code><br> | |<code>5'- CCGGATAGAGGGCTGCTGTATGCCCACTATAAG - 3'</code><br> | ||
|- | |- | ||
| + | | | ||
|EreB_SDMIII_for | |EreB_SDMIII_for | ||
|<code>5'- CGAGTTGATAACTTCCAACTACAGGAACCAAATGAAGGTTC - 3'</code><br> | |<code>5'- CGAGTTGATAACTTCCAACTACAGGAACCAAATGAAGGTTC - 3'</code><br> | ||
|- | |- | ||
| + | | | ||
|EreB_SDMIII_rev | |EreB_SDMIII_rev | ||
|<code>5'- GAACCTTCATTTGGTTCCTGTAGTTGGAAGTTATCAACTCG - 3'</code><br> | |<code>5'- GAACCTTCATTTGGTTCCTGTAGTTGGAAGTTATCAACTCG - 3'</code><br> | ||
Revision as of 17:32, 6 May 2013
Oligonucleotides
| Name | Sequence | |
| EreA_for | 5’- GCGCGTCTAGATGGCCGGCACGTGGAGAACGACCAGA -3‘ | |
| EreA-rev | 5‘- TTCGAAACCGGTTAGGGCAACTAGGCTGTCC -3‘ | |
| EreA_SDMI_for | 5’- CGAATCAGGAAGAAAACTACAGTTAGTCGGAATCGAC -3‘ | |
| EreA_SDMI_rev | 5'- GTCGATTCCGACTAACTGTAGTTTTCTTCCTGATTCG -3' | |
| EreA_SDMII_for | 5'- GACGCCTGTGGTACGGAAAATTCATGTCTGACATT - 3' | |
| EreA_SDMII_rev | 5'- AATGTCAGACATGAATTTTCCGTACCACAGGCGTC - 3' | |
| EreA_SDMIII_for | 5'- GTGTTCCAAGCGCGGGCAAGGACAGCC - 3' | |
| EreA_SDMIII_rev | 5'- GGCTGTCCTTGCCCGCGCTTGGAACAC - 3' | |
| EreB_for | 5'- GCGCGTCTAGATGGCCGGCAGGTTCGAAGAATGGGTC - 3' | |
| EreB-rev | 5'- TTCGAAACCGGTTTCATAAACGACCTCAGATACAG - 3' | |
| EreB_SDMI_for | 5'- GGTGAAAATTCTCATTTCATAAAAGAGTTCTTTTTGTTACGACATACGCTTTT - 3' | |
| EreB_SDMI_rev | 5'- AAAAGCGTATGTCGTAACAAAAAGAACTCTTTTATGAAATGAGAATTTTCACC - 3' | |
| EreB_SDMII_for | 5'- CTTATAGTGGGCATACAGCAGCCCTCTATCCGG - 3' | |
| EreB_SDMII_rev | 5'- CCGGATAGAGGGCTGCTGTATGCCCACTATAAG - 3' | |
| EreB_SDMIII_for | 5'- CGAGTTGATAACTTCCAACTACAGGAACCAAATGAAGGTTC - 3' | |
| EreB_SDMIII_rev | 5'- GAACCTTCATTTGGTTCCTGTAGTTGGAAGTTATCAACTCG - 3' | |
| TEV_fw | 5'- - 3' | |
| TEV_rv | 5'- - 3' | |
| TEV_t387c_fw | 5'- - 3' | |
| TEV_t387c_rv | 5'- - 3' | |
| N_split_TEV_fw | 5'- - 3' | |
| N_split_TEV_rv | 5'- - 3' | |
| C_split_TEV_fw | 5'- - 3' | |
| C_split_TEV_fw | 5'- - 3' | |
| PIF6_2-100_fw | 5'- - 3' | |
| PIF6_2-100_rv | 5'- - 3' | |
| MinMCS_fo | 5'- ctagacctaggaagcttcctgcaggactagtaagcttctgca - 3' | |
| MinMCS_rev | 5'- gaagcttactagtcctgcaggaagcttcctaggt - 3' | |
| Bpu_for | 5'- GCGCGTCTAGATGGCCGGCAACCTAGAAAAATTTGTTGACGAGCTG - 3' | |
| Bpu_rev | 5'- TTCGAAACCGGTGATGATATCCATCGGCCGCAT - 3' | |
| Bpu_SDMI_for | 5'- cacttaagcattttctacgggtcgatcacaccattca - 3' | |
| Bpu_SDMI_rev | 5'- tgaatggtgtgatcgacccgtagaaaatgcttaagtg - 3' | |
| Bpu_SDMII_fo | 5'- gacacgattaaatgtgtatgcgggcttagctggattttatttg - 3' | |
| Bpu_SDMII_re | 5'- caaataaaatccagctaagcccgcatacacatttaatcgtgtc - 3' | |
| Bpu_SDMIII_fo | 5'- cacccttaaaaataaagctggctgcggacaggaag - 3' | |
| Bpu_SDMIII_re | 5'- cttcctgtccgcagccagctttatttttaagggtg - 3' | |
| Bpu_SDMIV_fo | 5'- cgtacgctcactcttacgggtacacaggataaata - 3' | |
| Bpu_SDMIV_re | 5'- tatttatcctgtgtacccgtaagagtgagcgtacg - 3' | |
5'- - 3' | ||
5'- - 3' |
