Team:TU Darmstadt/materials/Buffers

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Lab book | Materials | Protocols

Buffers

Tris

  • Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C4H11NO3, 2-Amino-2-hydroxymethyl-propane-1,3-diol).


1x PBS

  • 8.18 g NaCl (140 mM)
  • 0.2 g KCl (2.7 mM)
  • 1.77 g Na2HPO4 (10 mM)
  • 0.24 g KH2PO4 (1.8 mM)
  • Add. 1L dH2O
  • Adjust to pH 7.4

TE buffer

  • 10 mM Tris, bring to pH 8.0 with HCl
  • 1 mM EDTA (Ethylenediaminetetraacetic acid)

50x TAE

  • 242g Tris base in water
  • add 57.1mL glacial acetic acid
  • 100mL of 500mM EDTA (pH 8.0) solution
  • add up to 1 litre of water