From 2013.igem.org
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| - | Table1: The growth rate k and the doubling time t<sub>d</sub> of BL21DE3 WT, BL21DE3 mKate and BL21DE3LSSmOrange.
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| - | <td><b>BL21DE3(pPR-IBA2)</b></td>
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| - | <td><b>BL21DE3[pPR-IBA2-LSSmOrange]</b></td>
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| - | <td><b>BL21DE3[pPR-IBA2-mKate]</b></td>
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| - | <th><b>growth rate µ [min-1]</b></th>
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| - | <td>0,0286</td>
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| - | <td>0,0287</td>
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| - | <td>0,0232</td>
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| - | <th><b>doubling time td [min]</b></th>
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| - | <td>24,2459</td>
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| - | <td>24,1514</td>
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| - | <td>29,877</td>
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| - | As can be seen in the two diagrams the course of the growth curves are rather similar. The growth rate as well as the doubling time of the wild type BL21DE3 and the one including the LSSmOrange construct are almost identical. The growth rate of the strain including the mKate construct is slightly lower and therefore the doubling time is around 5 min higher. All in all, the differences of the samples including the constructs compared to the wild type BL21DE2 are negligibly low. Therefore it can be assumed that the construct mKate as well as LSSmOrange are not toxic for the BL21DE3 cells and have no or a negligibly low influence on the growth of the cells.
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Revision as of 01:09, 5 October 2013
Electrical engineering
Handheld development:
blabblabalalbal [1] showed that LSSmOrange has an excitation maximum by
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LED Sensing:
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App development:
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Bluetooth communication:
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References
- Daria M. Shcherbakova et al. (2012) An Orange Fluorescent Protein with a Large Stokes Shift for Single-Excitation Multicolor FCCS and FRET Imaging. J. Am. Chem. Soc. 134 (18), 7913–7923
- http://www.evrogen.com/products/basicFPs.shtml
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