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| - | <h1>Overview</h1><h2>A love story contains several processes. Two people fall in love and their love burning wildly. However, no forever exists in the world, in most occasions, love will eventually burn to only a pile of ashes of the last remaining wind drift away. In our project, we have recreated the story of "Romeo and Juliet" by Shakespeare vividly by two kinds of Escherichia coli cell-cell communication. We aim to generate a circuit involving regulatory mechanism of positive feedback rather than commonly-used negative feedback to control the fate of E.coli by signaling between two types of E.coli. Besides, Rose represents love. We are the first iGEM group ever to synthesize P(3HB) (a kind of bio-plastics) by using our new biobrick part, representing rose . | + | <h1>Abstract</h1><h2>Food shortages are said to be inevitable due to rapid population growth. Previous iGEM teams have approached to solve the problem by challenging to express plant hormone in E.Coli (2010 imperial college, 2012 Evry, 2012 Kyoto). |
| - | Ⅰ cell-cell communication
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| - | "Romeo and Juliet" is the drama by dramatist William Shakespeare of England. The stage is city Verona, Italy in the 14th century. Romeo met Juliet. Two people fell in love instantly. In this project, we will recreate the love story of "Romeo and Juliet".</h2>
| + | Our goal is to make progress with transforming E. Coli to act upon plants. That is to say, we aim to create “Farmer E. Coli”, which sense the nutrition of soil and express the plant hormones properly. We need to synthesize Auxin, which is presented by Imperial College in 2010 iGEM, and Cytokinin on our own. |
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| | + | We will find the solution of “Safety” and “Crosstalk” problems considered when we put this to practical use. As to safety, we plan to create E. Coli which destroys external E. Coli by genome transportation of phage. To avoid crosstalk, we will construct circuits, which have been our team’s specialty. |
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Abstract
Food shortages are said to be inevitable due to rapid population growth. Previous iGEM teams have approached to solve the problem by challenging to express plant hormone in E.Coli (2010 imperial college, 2012 Evry, 2012 Kyoto).
Our goal is to make progress with transforming E. Coli to act upon plants. That is to say, we aim to create “Farmer E. Coli”, which sense the nutrition of soil and express the plant hormones properly. We need to synthesize Auxin, which is presented by Imperial College in 2010 iGEM, and Cytokinin on our own.
We will find the solution of “Safety” and “Crosstalk” problems considered when we put this to practical use. As to safety, we plan to create E. Coli which destroys external E. Coli by genome transportation of phage. To avoid crosstalk, we will construct circuits, which have been our team’s specialty.
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