Team:USTC CHINA

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                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview" target="_blank">Overview</a></li>
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                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/ReporterSystem" target="_blank">Reporter System</a></li>
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                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication" target="_blank">Communication</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Safety">Safety</a></li>
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<h1>In Situ Transdermal Vaccine</h1></div>
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<p>Fresh Expression, No Purification<br />Standardization, Block-based design<br />Easy-transportation, Low Storage External Costs<br />
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Almost No Demand For Medical Conditions And Proferssionals<br /></p>
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<p></p><p><a class="button-home" href="https://2013.igem.org/Team:USTC_CHINA/Project"><span>Project Details</span></a></p></div>
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<h1>Why We Begin</h1>
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  we hope to provide a needle-free and non-skin-damage vaccination,
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  <br />we hope everyone, even in the least developed area, could enjoy the benefit of medical progress.<br />
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Background">more about project background</a>
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<h1>How We Design</h1>
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  With the support of <span>TD-1</span>, a special polypeptide, which can greatly facilitate macromolecule transdermal delivery through intact skin, we construct three different fuctional engineered bacillus subtilis; besides, we design a kill switch in the fouth type B.subtilis to ensure biosafety.</div>
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  <span>ANTIGEN</span><br />
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<li><a href="#"><span>overview</span></a></li>
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As the core of our in-situ system,<br /> TD1-antigen expresser is designed<br /> and will share a great percentage of<br /> the total bacteria in our "band-aid".
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Design">more about project design</a>
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  <span>LTB</span><br />
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As one kind of our adjuvant expresser,<br />TD1-LTB is used to enhance the antigenicity.<br /> For LTB has so many advantages, it's a wild using adjuvant for most of exsiting injected vaccine.
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Design">more about project design</a>
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  <span>KNFα</span><br />
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It's worth mentioning that to improve the effects of <br />traditional vaccine, as another kind of our TD1-adjuvant expresser, KNFα bear the weight of our hope to increase the sensitivity of our immune system.
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<li><a href="https://2013.igem.org/Team:USTC_CHINA/team"><span>about us</span></a></li>
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Design">more about project design</a>
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  <span>REPORTER</span><br />
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Reporter, which notifies users whether the status of vaccine patch is all right and when they can stick the patches to arms .  Further more killing switch is existed in them as a biosafety guard.
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Design">more about project design</a>
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<h1>What We've Achived</h1>
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<span>Molecules part</span><br />
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We took E.coli and the PET vector as the positive control and realized our ideas in the B.subtilis expression system based on PHT43 shuttle vector.<br />
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">more about project results</a>
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  <span>Protein part</span><br />
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  Before we achieve fresh expression, we need first use purified protein to obtain exact data to verify our assumption.<br />
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Results">more about project results</a>
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  <span>Mice experiments</span><br />
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  The most obvious character of our 2013USTC iGEM team is that we have not only achieved a series of molecule experiments but also tried mice experiments, which could be divided into transdermal experiments in vitro and animate mice experiments<br />
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">more about project results</a>
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<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture"><h2>Suicide mechanism of the reporter system</h2>
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<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/ReporterSystem" ><h2>The optimal culture conditions of Bacillus subtilis</h2>
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<h2>Mice Modeling</h2></a>
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<div id="content1p">Project Description</div>
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<h1>Human Practice</h1>
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<div id="content2p"><p>Based on the fundamental principles and technology of synthetic biology, our team aims at designing a new immune system containing transdermal peptide that expresses genes and produces immune responses simultaneously. To reach this goal, we need to build four kinds of engineered bacteria and find out effective immune responses. Meanwhile, an appropriate reporting system guiding vaccination at different stages is also required to meet the demand of practical, safe and human-friendly production. The Bacillus subtilis we use is WB800N, which is modified into four types of engineered bacteria, combined with the gene coding LTB, HBsAg, reporter, and LC Luman-recruiting factor respectively, each of which contains the gene coding transdermal peptide. Then, the engineered bacteria will produce mixture of fusion protein in the skin and activate immune responses.</p>
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<p> The introducing of transdermal peptide, together with the simultaneous immune response with protein expression, has granted our work great advantages over other existing immune system, both in economy and operation. Therefore it is expected to become a promising new vaccine research field.</P>
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<h2>Communication</h2>
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<p> So far, we have successfully synthesize four kinds of plasmids, and the sequences of them all have been proved correct. Additionally, we have managed to transform three plasmids from E. coli to Bacillus subtilis, leaving GFP in progress. </p>
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<img src="images/china-map.png" width="310" height="259" /></a>
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  <p> For the coming days, we plan to continue our transformation and experiment on the mice to check the effects of our vaccine, whereas other tasks, such as human practice and the standardization of our parts, are in our future schedule.</p></div>
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<h2>Activity</h2>
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     <h3 align="center" id="sponsors-title"><em>Sponsors</em></h3><br>
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     <h3 id="sponsors-title" align="center"><em>Thanks to the sponsors</em></h3><br>
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       <p align="center">Deutsche Bank, China</p>
       <p align="center">Deutsche Bank, China</p>
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         <div align="center"><a href="https://china.db.com/index_e.html" target="_blank"><img src="https://static.igem.org/mediawiki/2012/f/fc/USTC-Software-images-db-logo.png" alt="Deutsche Bank, China" width="280px" height="65px" align="absmiddle"></a>
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         <div align="center"><a href="https://china.db.com/index_e.html" target="_blank"><img src="images/USTC-Software-images-db-logo.png" alt="Deutsche Bank, China" height="65px" align="absmiddle" width="280px"></a>
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         <p align="center">School of Life Sciences, USTC</p>
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Revision as of 15:36, 21 September 2013

USTC_China

In Situ Transdermal Vaccine

Fresh Expression, No Purification
Standardization, Block-based design
Easy-transportation, Low Storage External Costs
Almost No Demand For Medical Conditions And Proferssionals

Project Details

Why We Begin


we hope to provide a needle-free and non-skin-damage vaccination,
we hope everyone, even in the least developed area, could enjoy the benefit of medical progress.
more about project background

How We Design


With the support of TD-1, a special polypeptide, which can greatly facilitate macromolecule transdermal delivery through intact skin, we construct three different fuctional engineered bacillus subtilis; besides, we design a kill switch in the fouth type B.subtilis to ensure biosafety.

ANTIGEN
As the core of our in-situ system,
TD1-antigen expresser is designed
and will share a great percentage of
the total bacteria in our "band-aid".
more about project design


LTB
As one kind of our adjuvant expresser,
TD1-LTB is used to enhance the antigenicity.
For LTB has so many advantages, it's a wild using adjuvant for most of exsiting injected vaccine.
more about project design


KNFα
It's worth mentioning that to improve the effects of
traditional vaccine, as another kind of our TD1-adjuvant expresser, KNFα bear the weight of our hope to increase the sensitivity of our immune system.
more about project design


REPORTER
Reporter, which notifies users whether the status of vaccine patch is all right and when they can stick the patches to arms . Further more killing switch is existed in them as a biosafety guard.
more about project design

What We've Achived



Molecules part
We took E.coli and the PET vector as the positive control and realized our ideas in the B.subtilis expression system based on PHT43 shuttle vector.
more about project results


Protein part
Before we achieve fresh expression, we need first use purified protein to obtain exact data to verify our assumption.
more about project results


Mice experiments
The most obvious character of our 2013USTC iGEM team is that we have not only achieved a series of molecule experiments but also tried mice experiments, which could be divided into transdermal experiments in vitro and animate mice experiments
more about project results

Retrieved from "http://2013.igem.org/Team:USTC_CHINA"