Team:USTC CHINA/Notebook/Protocols

From 2013.igem.org

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<h>Gene Clone</h>
<h>Gene Clone</h>
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<h1>Gel Extraction</h1>
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<a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Gel Extraction"><h1>Gel Extraction</h1></a>
<h1>DNA digestion</h1>
<h1>DNA digestion</h1>
<h2>Single Digestion</h2>
<h2>Single Digestion</h2>

Revision as of 11:40, 25 September 2013

Gene Clone

Gel Extraction

DNA digestion

Single Digestion

Double Digestion

Colony PCR

PCR

Ligation

Plasmid mini-prep

Transformation

The transformation of Bacillus subtilis

Overlap PCR

Extracting plasmids

Extracting plasmids from gram-positive bacterium

Extracting plasmids from gram-negative bacterium

Expression of proteins

Expression of proteins in Bacillus subtilis

Expression of proteins in Escherichia coli

Concentrating proteins

Transdermal experiments
Immunity&Tests

ELISA

Sample analysis

Medium

LB media

10*S base

HS media

LS media

2*YT media

Measuremen

Constitutive promoter measurements

plac promoter response to IPTG or Glucose

notebook

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