Team:USTC CHINA/Notebook/Protocols

From 2013.igem.org

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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Gel Extraction">Gel Extraction</a></h1>
<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Gel Extraction">Gel Extraction</a></h1>
<h1>DNA digestion</h1>
<h1>DNA digestion</h1>
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<h2>Single Digestion</h2>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Single Digestion">Single Digestion</a></h2>
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<h2>Double Digestion</h2>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Double Digestion">Double Digestion</a></h2>
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<h1>Colony PCR</h1>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Colony PCR">Colony PCR</a></h1>
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<h1>PCR</h1>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/PCR">PCR</a></h1>
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<h1>Ligation</h1>
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Ligation">Ligation</a></h1>
<h1>Plasmid mini-prep</h1></div>
<h1>Plasmid mini-prep</h1></div>
<div class="protocol-col-sub">
<div class="protocol-col-sub">

Revision as of 12:51, 25 September 2013

Gene Clone

Gel Extraction

DNA digestion

Single Digestion

Double Digestion

Colony PCR

PCR

Ligation

Plasmid mini-prep

Transformation

The transformation of Bacillus subtilis

Overlap PCR

Extracting plasmids

Extracting plasmids from gram-positive bacterium

Extracting plasmids from gram-negative bacterium

Expression of proteins

Expression of proteins in Bacillus subtilis

Expression of proteins in Escherichia coli

Concentrating proteins

Transdermal experiments Immunity&Tests

ELISA

Sample analysis

Medium

LB media

10*S base

HS media

LS media

2*YT media

Measuremen

Constitutive promoter measurements

plac promoter response to IPTG or Glucose

notebook

Retrieved from "http://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols"