Team:USTC CHINA/Notebook/Protocols

From 2013.igem.org

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<h>Measuremen</h>
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<h>Measurement</h>
<h1>Constitutive promoter measurements</h1>
<h1>Constitutive promoter measurements</h1>
<h1>plac promoter response to IPTG or Glucose</h1>
<h1>plac promoter response to IPTG or Glucose</h1>

Revision as of 12:52, 25 September 2013

Gene Clone

Transformation

The transformation of Bacillus subtilis

Overlap PCR

Extracting plasmids

Extracting plasmids from gram-positive bacterium

Extracting plasmids from gram-negative bacterium

Expression of proteins

Expression of proteins in Bacillus subtilis

Expression of proteins in Escherichia coli

Concentrating proteins

Transdermal experiments Immunity&Tests

ELISA

Sample analysis

Medium

LB media

10*S base

HS media

LS media

2*YT media

Measurement

Constitutive promoter measurements

plac promoter response to IPTG or Glucose