Team:USTC CHINA/Notebook/Protocols/Expression of proteins in Bacillus subtilis

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- grow appropriate B. subtilis strain overnight in fresh 2xYT medium</br>
- grow appropriate B. subtilis strain overnight in fresh 2xYT medium</br>
- inoculate into fresh 2xYT medium to an OD600 of 0.15</br>
- inoculate into fresh 2xYT medium to an OD600 of 0.15</br>
-
- when culture reaches OD600 0.7 – 0.8, split into 2 portions and induce with 1 mM IPTG(CB-0487-1GAM) to one portion (t = 0)</br>
+
- when culture reaches OD600 0.7 – 0.8, split into 2 portions and induce with 1 mM </br>  IPTG(CB-0487-1GAM) to one portion (t = 0)</br>
- collect samples at different time points for analysis (t = 1,…)</br>
- collect samples at different time points for analysis (t = 1,…)</br>
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Revision as of 15:01, 25 September 2013

Expression of proteins in Bacillus subtilis

Induction with IPTG
- grow appropriate B. subtilis strain overnight in fresh 2xYT medium
- inoculate into fresh 2xYT medium to an OD600 of 0.15
- when culture reaches OD600 0.7 – 0.8, split into 2 portions and induce with 1 mM
IPTG(CB-0487-1GAM) to one portion (t = 0)
- collect samples at different time points for analysis (t = 1,…)