Team:USTC CHINA/Notebook/Protocols/Gel Extraction

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Add 0.5 × Buffer DE-A volume of Buffer DE-B.</br>
Add 0.5 × Buffer DE-A volume of Buffer DE-B.</br>
   
   
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3. Binding sample DNA(Centrifuge at 5,000 - 6,000×g for 5 min)</br>
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3. Binding sample DNA(Centrifuge at 5,000 - 6,000×g for 5 min).</br>
   
   
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4. Add 800 μl of Buffer W2 (5,000 - 6,000×g for 1 min)
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4. Add 800 μl of Buffer W2 (5,000 - 6,000×g for 1 min).</br>
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Repeat wash with Buffer W2</br>
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Repeat wash with Buffer W2.</br>
Centrifuge empty plate for 5 min at 5,000 - 6,000×g to remove residue W2.</br>
Centrifuge empty plate for 5 min at 5,000 - 6,000×g to remove residue W2.</br>
   
   
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5. Elute with 50 μl of Eluent or Deionized Water.(5,000 - 6,000×g, 5min)</br>
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5. Elute with 50 μl of Eluent or Deionized Water.(5,000 - 6,000×g, 5min).</br>
   
   

Revision as of 16:11, 25 September 2013

Gel Extraction

Performed with AxyPrep 96-well DNA Gel Extraction Kit (type: AP-96-GX) Protocol 1. Excise gel slice containing DNA fragment of interest.
2. Add 3×sample volume of Buffer DE-A.
Incubate at 75° C for 15-20 min or until gel melts completely. Add 0.5 × Buffer DE-A volume of Buffer DE-B.
3. Binding sample DNA(Centrifuge at 5,000 - 6,000×g for 5 min).
4. Add 800 μl of Buffer W2 (5,000 - 6,000×g for 1 min).
Repeat wash with Buffer W2.
Centrifuge empty plate for 5 min at 5,000 - 6,000×g to remove residue W2.
5. Elute with 50 μl of Eluent or Deionized Water.(5,000 - 6,000×g, 5min).

notebook

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