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Team:Washington/HOMEPAGE PROTOCOL
From 2013.igem.org
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<li><a href = "https://2013.igem.org/Team:Washington/LB_AND_TB_MEDIA">LB and TB Media</a></li> | <li><a href = "https://2013.igem.org/Team:Washington/LB_AND_TB_MEDIA">LB and TB Media</a></li> | ||
<li><a href = "https://2013.igem.org/Team:Washington/POURING_AGAR_PLATES">Pouring Agar Plates</a></li> | <li><a href = "https://2013.igem.org/Team:Washington/POURING_AGAR_PLATES">Pouring Agar Plates</a></li> | ||
| + | </ul> | ||
| + | </p> | ||
| + | |||
| + | <p> | ||
| + | Instruments: | ||
| + | <ul> | ||
| + | <li><a href = "https://2013.igem.org/Team:Washington/NANODROP_PROTOCOL">Nanodrop Protocol</a></li> | ||
| + | </ul> | ||
| + | </p> | ||
| + | |||
| + | <p> | ||
| + | See also: | ||
| + | <ul> | ||
| + | <li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BIOTECHNOLOGY_INFORMATION">General Biotechnology Information</a></li> | ||
| + | <li><a href = "https://2013.igem.org/Team:Washington/SOS_LAB_PROTOCOLS">SOS Lab Protocols</a></li> | ||
| + | <li><a href = "http://ginkgobioworks.com/support/BioBrick_Assembly_Manual.pdf">http://ginkgobioworks.com/support/BioBrick_Assembly_Manual.pdf</a></li> | ||
</ul> | </ul> | ||
</p> | </p> | ||
Revision as of 02:08, 7 September 2013
Tips and Etiquette:
Keep notebooks up-to-dateWorkflow:
- Primer Design
- Streaking a Plate for Isolation
- Overnight Cultures
- 1) Isolation of Plasmid DNA (miniprep)
- 2) General PCR Protocol (also see PCR GoTag - product (30-200ng/ ul) Check on gel PCR Purification and/or Dpnl Digest
- 3) General Digestion Protocol Check on gel PCR Purification or Heat Inactivation (check enzyme temp and time, usually 80C for 20min)
- 4) Agarose Gel Electrophoresis
- 5) General Ligation Protocol (Don’t forget background control plates) Heat Inactivation (optional - up to 10 fold increase) - 65° for 10 minutes
- 6) Heat shock/chemical competent transformation
- 7) Colony PCR with Green taq Miniprep (stocks can be made from this culture - add 1ml extra)
- 8) DNA Sequencing
- 9) Making Glycerol Frozen Stocks
Media Preparation:
Instruments:
See also:
- General Biotechnology Information
- SOS Lab Protocols
- http://ginkgobioworks.com/support/BioBrick_Assembly_Manual.pdf
