Team:Washington/HOMEPAGE PROTOCOL

From 2013.igem.org

(Difference between revisions)
 
(22 intermediate revisions not shown)
Line 2: Line 2:
<header>
<header>
-
<u>Cloning Protocols Workflow</u>
+
<b><u>Cloning Protocols Workflow</u></b>
</header>
</header>
Line 10: Line 10:
<p>
<p>
-
Tips and Etiquette:
+
<b>Tips and Etiquette:</b>
<ul>
<ul>
-
<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">Tab 1</a></li>
+
<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">General Basic Protocol Information</a></li>
-
<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">Tab 2</a></li>
+
<li><a href = "https://2013.igem.org/Team:Washington/HOW_TO_LABEL_EVERYTHING">How to Label EVERYTHING</a></li>
</ul>
</ul>
Keep notebooks up-to-date
Keep notebooks up-to-date
Line 19: Line 19:
<br>
<br>
-
<p1>
 
-
I AM PARAGRPAHIRGJIG 2
 
-
</p1>
 
 +
<p>
 +
<b>Workflow:</b>
 +
<ul>
 +
<li><a href = "https://2013.igem.org/Team:Washington/PRIMER_DESIGN">Primer Design</a></li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/STREAKING_A_PLATE">Streaking a Plate for Isolation</a></li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/OVERNIGHT_CULTURES">Overnight Cultures</a></li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/ISOLATION_OF_PLASMID">1) Isolation of Plasmid DNA (miniprep)</a></li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_PCR_PROTOCOL">2) General PCR Protocol </a>(also see <a href = "https://2013.igem.org/Team:Washington/PCR_GOTAG">PCR GoTag</a> - product (30-200ng/ ul) Check on gel PCR Purification and/or  <a href = "https://2013.igem.org/Team:Washington/DPNL_DIGEST">Dpnl Digest</a></li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_DIGESTION_PROTOCOL">3) General Digestion Protocol</a> Check on gel PCR Purification or Heat Inactivation (check enzyme temp and time, usually 80C for 20min)</li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/AGAROSE_GEL">4) Agarose Gel Electrophoresis</a></li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_LIGATION_PROTOCOL">5) General Ligation Protocol</a> (Don’t forget background control plates) Heat Inactivation (optional - up to 10 fold increase) - 65° for 10 minutes</li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/HEAT_SHOCK_CHEM_TRANS">6) Heat shock/chemical competent transformation</a></li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/COLONY_PCR_GREEN">7) Colony PCR with Green taq</a> Miniprep (stocks can be made from this culture - add 1ml extra)</li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/DNA_SEQUENCING">8) DNA Sequencing</a></li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/MAKING_GLYCEROL_FROZEN_STOCKS">9) Making Glycerol Frozen Stocks</a></li>
 +
</ul>
 +
</p>
 +
 +
<br>
 +
 +
<p>
 +
<b>Media Preparation:</b>
 +
<ul>
 +
<li><a href = "https://2013.igem.org/Team:Washington/COMPETENT_CELL_STOCKS">Competent Cell Stocks</a></li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/LB_AND_TB_MEDIA">LB and TB Media</a></li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/POURING_AGAR_PLATES">Pouring Agar Plates</a></li>
 +
</ul>
 +
</p>
 +
 +
<br>
 +
 +
<p>
 +
<b>Instruments:</b>
 +
<ul>
 +
<li><a href = "https://2013.igem.org/Team:Washington/NANODROP_PROTOCOL">Nanodrop Protocol</a></li>
 +
</ul>
 +
</p>
 +
 +
<br>
 +
 +
<p>
 +
<b>See also:</b>
 +
<ul>
 +
<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BIOTECHNOLOGY_INFORMATION">General Biotechnology Information</a></li>
 +
<li><a href = "https://2013.igem.org/Team:Washington/SOS_LAB_PROTOCOLS">SOS Lab Protocols</a></li>
 +
<li><a href = "http://ginkgobioworks.com/support/BioBrick_Assembly_Manual.pdf">http://ginkgobioworks.com/support/BioBrick_Assembly_Manual.pdf</a></li>
 +
</ul>
 +
</p>
</body>
</body>
</html>
</html>

Latest revision as of 02:11, 7 September 2013

Cloning Protocols Workflow

Tips and Etiquette:

Keep notebooks up-to-date


Workflow:


Media Preparation:


Instruments:


See also:

Retrieved from "http://2013.igem.org/Team:Washington/HOMEPAGE_PROTOCOL"