Team:Yale/Project Export

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<div id="cssmenu1" style="width:830px; margin:0 auto;">
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<ul>
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  <li><a href='https://2013.igem.org/Team:Yale/Project_Overview'><span>Project Overview</span></a></li>
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  <li><a href='https://2013.igem.org/Team:Yale/Project_Validate'><span>Validate PLA Synthesis</span></a></li>
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  <li><a href='https://2013.igem.org/Team:Yale/Project_Bioassay'><span>Develop Bioassay</span></a></li>
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  <li><a href='https://2013.igem.org/Team:Yale/Project_MAGE'><span>Apply Mage</span></a></li>
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  <li class='active'><a href='https://2013.igem.org/Team:Yale/Project_Export'><span>Introduce Export System</span></a></li>
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  <li><a href='https://2013.igem.org/Team:Yale/Project_Bioplastic'><span>Make a Bioplastic</span></a></li>
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<li class='last'><a href='https://2013.igem.org/Team:Yale/Project_Collaboration'><span>Collaboration</span></a></li>
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</div>
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{| class = "bar" style="color:#3399CC;background-color:#737CA1;" cellpadding="2" cellspacing="1" border="1" bordercolor="#fff" width="75%" align="center"
 
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!align="center" style="background-color:#FFFFFF;" cellpadding="0" cellspacing="0" border="0"|<html><a href="https://2013.igem.org/Team:Yale/Project_MAGE"><img src="https://encrypted-tbn3.gstatic.com/images?q=tbn:ANd9GcTVb8adyUdr2p_7aBWNSMfB83hCbKIaQhdkJ_k21JZ4PXnle8w8" style="-moz-transform: scaleX(-1); -webkit-transform: scaleX(-1); -o-transform: scaleX(-1); transform: scaleX(-1); -ms-filter: fliph; /*IE*/ filter: fliph; /*IE*/" width="50" height="50" title="Previous: Apply MAGE" /></a></html>
 
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!align="center"|[[Team:Yale/Project_Overview|Project Overview]]
 
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!align="center"|[[Team:Yale/Project_Validate|Validate PLA synthesis]]
 
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!align="center"|[[Team:Yale/Project_Bioassay|Develop bioassay]]
 
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!align="center"|[[Team:Yale/Project_MAGE|Apply MAGE]]
 
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!align="center"|[[Team:Yale/Project_Export|Introduce export system]]
 
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!align="center"|[[Team:Yale/Project_Bioplastic|Make a bioplastic]]
 
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!align="center" style="background-color:#FFFFFF;" cellpadding="0" cellspacing="0" border="0"|<html><a href="https://2013.igem.org/Team:Yale/Project_Bioplastic"><img src="https://encrypted-tbn3.gstatic.com/images?q=tbn:ANd9GcTVb8adyUdr2p_7aBWNSMfB83hCbKIaQhdkJ_k21JZ4PXnle8w8" width="50" height="50" title="Next: Make a bioplastic" /></a></html>
 
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|}
 
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=== Introduce type 1 secretion system to export and extract PLA ===
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== Aims for the Project ==
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*We needed a way to export the PLA once it was synthesized by the ''E. coli''
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#'''Engineer strains of ''E. coli'' to validate PLA synthesis'''<br>
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**We found the paper by Linton 2010 where she focused on exporting PHA from engineered ''E. coli''
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#'''Develop bioassay to screen PLA production'''<br>
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#'''Apply MAGE to optimize PLA production, guided by FBA'''<br>
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#'''<span style="color:red">Introduce type 1 secretion system to export and extract PLA</span>'''<br>
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#'''Make a bioplastic'''
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<br>
<br>
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*Ms. Linton used Phasin, a PHA granule associated protein that plays a role in granule formation, with a hlyA tag. 
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=== Introduce type 1 secretion system to export and extract PLA ===
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**This allowed the cells to export the PLA since the hlyA tag was attached to the granule
<center>[[File:PHA_export_system.jpg|400px]]</center>
<center>[[File:PHA_export_system.jpg|400px]]</center>
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<br><br>
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*Due to the similarity between PHA granules and PLA granules we hypothesized that this same export system would allow us to export PLA from our cells.
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<center>[[File:PLAandPHAexport.png|600px]]</center>

Latest revision as of 00:23, 27 September 2013


Introduce type 1 secretion system to export and extract PLA

  • We needed a way to export the PLA once it was synthesized by the E. coli
    • We found the paper by Linton 2010 where she focused on exporting PHA from engineered E. coli


  • Ms. Linton used Phasin, a PHA granule associated protein that plays a role in granule formation, with a hlyA tag.
    • This allowed the cells to export the PLA since the hlyA tag was attached to the granule
PHA export system.jpg



  • Due to the similarity between PHA granules and PLA granules we hypothesized that this same export system would allow us to export PLA from our cells.
PLAandPHAexport.png
Retrieved from "http://2013.igem.org/Team:Yale/Project_Export"