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Team:Yale/Project Export
From 2013.igem.org
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=== Introduce type 1 secretion system to export and extract PLA === | === Introduce type 1 secretion system to export and extract PLA === | ||
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| + | *We needed a way to export the PLA once it was synthesized by the ''E. coli'' | ||
| + | **We found the paper by Linton 2010 where she focused on exporting PHA from engineered ''E. coli'' | ||
| + | <br> | ||
| + | *She used Phasin, a PHA granule associated protein that plays a role in granule formation, with a hlyA tag. This allowed the cells to export the PLA since the hlyA tag was attached to the granule | ||
<center>[[File:PHA_export_system.jpg|400px]]</center> | <center>[[File:PHA_export_system.jpg|400px]]</center> | ||
| + | <br><br> | ||
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| + | *Due to the similarity between PHA granules and PLA granules we hypothesized that this same export system would allow us to export PLA from our cells. | ||
| + | <center>[[File:PLAandPHAexport.png|600px]]</center> | ||
Revision as of 20:43, 31 August 2013
| Project Overview | Validate PLA synthesis | Develop bioassay | Apply MAGE | Introduce export system | Make a bioplastic |
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Aims for the Project
- Engineer strains of E. coli to validate PLA synthesis
- Develop bioassay to screen PLA production
- Apply MAGE to optimize PLA production, guided by FBA
- Introduce type 1 secretion system to export and extract PLA
- Make a bioplastic
Introduce type 1 secretion system to export and extract PLA
- We needed a way to export the PLA once it was synthesized by the E. coli
- We found the paper by Linton 2010 where she focused on exporting PHA from engineered E. coli
- She used Phasin, a PHA granule associated protein that plays a role in granule formation, with a hlyA tag. This allowed the cells to export the PLA since the hlyA tag was attached to the granule
- Due to the similarity between PHA granules and PLA granules we hypothesized that this same export system would allow us to export PLA from our cells.
