Team:Yale/Project Validate

From 2013.igem.org

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{| class = "bar" style="color:#3399CC;background-color:#737CA1;" cellpadding="2" cellspacing="1" border="1" bordercolor="#fff" width="75%" align="center"
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!align="center"|[[Team:Yale/Project_Overview|Project Overview]]
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!align="center"|[[Team:Yale/Project_Validate|Validate PLA synthesis]]
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!align="center"|[[Team:Yale/Project_Bioassay|Develop bioassay]]
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!align="center"|[[Team:Yale/Project_MAGE|Apply MAGE]]
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!align="center"|[[Team:Yale/Project_Export|Introduce export system]]
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!align="center"|[[Team:Yale/Project_Bioplastic|Make a bioplastic]]
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|}
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== Aims for the Project ==
== Aims for the Project ==
#'''Engineer strains of ''E. coli'' to validate PLA synthesis'''<br>
#'''Engineer strains of ''E. coli'' to validate PLA synthesis'''<br>

Revision as of 01:08, 22 August 2013

Project Overview Validate PLA synthesis Develop bioassay Apply MAGE Introduce export system Make a bioplastic

Aims for the Project

  1. Engineer strains of E. coli to validate PLA synthesis
  2. Develop bioassay to screen PLA production
  3. Apply MAGE to optimize PLA production, guided by FBA
  4. Introduce type 1 secretion system to export and extract PLA
  5. Make a bioplastic


Engineer strains of E. coli to validate PLA synthesis

Step 1 outline.jpg This was our plan in order to validate PLA synthesis. In order to save money we ordered each of the two heterologous genes in four pieces from IDT giving us 8 GBlocks.